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Citations for BioLegend :
351 - 396 of 396 citations for 7 12 Dioxa spiro 5.6 dodec 9 ene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The hybridoma sample was stained with a 12 μg/mL solution of TotalSeqC conjugated anti IgG1 antibody (Biolegend) and incubated for 45 minutes on Ice ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Immunology 2022Quote: ... Cytokines in the cell culture supernatants were quantified using Human Th Cytokine Panel (12-plex) (Cat. 741027, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were treated with 81 nM phorbol 12-myristate 13-acetate (PMA) plus 1.34 µM Ionomycin (BioLegend, 423301) for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... were stimulated with 500x Cell Activation Cocktail containing 40.5 μM phorbol-12-myristate 13-acetate (PMA) and 669.3 μM ionomycin (Biolegend) in the presence of 5 μg/mL BFA (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with cell activation cocktail (including phorbol 12-myristate 13-acetate and Ionomycin) (BioLegend) in the presence of Golgi Stop (monensin ...
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bioRxiv - Immunology 2022Quote: Ten 10-week-old male wild type C57BL/6J mice were intravenously injected with an APC/Cyanine 7-conjugated anti-mouse CD45.2 antibody (Clone 104, BioLegend 109824, RRID: AB_830789) five minutes prior to euthanasia by CO2 inhalation ...
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human IL-2 ELISA MAX Deluxe kit and Human IL-12 (p70) ELISA MAX Deluxe kit in accordance with the manufacturer’s instructions (BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 6-18 (Tau 12, 1:1,000 dilution, ref: 806501, Biolegend), 159–163 (Tau HT7 ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell RNAseq data for the 7 PDA cell lines were generated as following: Cell lines were trypsinized and resuspended in Cell Staining Buffer (Biolegend, Cat. No. 420201) and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Cancer Biology 2021Quote: ... NKG2D ligands were blocked on the cancer cell lines and single cells of tumor organoids by incubating the target cells with 12 µg/mL anti-MICA/B (clone 6D4, BioLegend), 1 µg/mL anti-ULBP1 (clone 170818 ...
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bioRxiv - Genomics 2020Quote: ... 1×105 TILs were seeded per well of a round bottom 96 well plate and stimulated with phorbol-12-myristate-13-acetate/Ionomycin in the presence of Brefeldin A (Biolegend) for 4 hours as per manufacturer’s instruction ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained on ice for 30 min with the following fluorochrome-conjugated antibodies in a 12-color staining combination: APC anti-IL17RA (clone W15177A, BIolegend) at 1:50 ...
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bioRxiv - Immunology 2020Quote: ... Supernatant from eleven donors responding in the ELISPOT assay were profiled using a 12-plex Legendplex T Helper Cytokine Panel Version 2 (Biolegend) following the manufactures instructions ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2019Quote: ... isolated lymphocytes were first restimulated with 5 ng/ml phorbal 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin in the presence of monensin (Biolegend) for 3.5 hours ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured in stimulation media without peptide stimulation or with phorbol 12-myristate 13-acetate (PMA) and ionomycin (BioLegend), respectively ...
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bioRxiv - Immunology 2022Quote: ... 50,000 CD4+ T cells were added to each well of mBECs in 12-well chamber slides in Hank’s balanced salt solution (HBSS) with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
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bioRxiv - Immunology 2022Quote: ... isolated lymphocytes were first restimulated with 5 ng/mL phorbal 12-myristate 13-acetate and 500 ng/mL ionomycin in the presence of monensin (Biolegend) for 3.5 h ...
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bioRxiv - Physiology 2022Quote: ... CD11b+-SVF were incubated with were with 0.2 mg/mL of anti-mouse PE-F4/80 (BioLegend, #12-4801-82) with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80 ...
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bioRxiv - Immunology 2023Quote: ... anti-CD25(Percp-Cy5.5, #302626), anti-CD69(APC, #310910), anti-mouse CD25(PE, #12-0251-83), anti-mouse CD69(FITC, #104506) from Biolegend, anti-human IgG(SureLight® PE ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
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bioRxiv - Cell Biology 2023Quote: ... and cultured in DMEM/F-12 medium containing 10% FBS and 20 ng/mL mouse macrophage colony-stimulating factor (BioLegend) for 7 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Murine anti-V5-tag (clone 7/4, #680602) and rabbit anti-V5-tag (Polyclonal, #903801) antibodies were purchased from BioLegend (San Diego, CA, USA). Anti-V5-tag mAb-magnetic beads (#M167-11 ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1-PE-Cy7 and FcγR-PE (eBioscience, 12-0161-83) for myeloid differentiation, or Mac-1-APC (eBioscience, 17-0112-82) and CD19-PB (BioLegend, 115523) for lymphoid differentiation on OP9 cells ...
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bioRxiv - Immunology 2020Quote: The serum levels of a total of 12 virus-related cytokines were measured by a bead-based immunoassay panel (Mouse Anti-Virus Panel, Cat No: 740622, Biolegend, USA). The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel ...
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bioRxiv - Immunology 2020Quote: ... The BALF levels of a total of 12 inflammatory cytokines were measured by a bead-based immunoassay panel (Mouse Inflammation Panel, Cat No: 740446, Biolegend, USA) on CytoFLEX Flow Cytometer (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... PE-conjugated anti-IL-17 (1:30, eBioscience Cat# 12-7177-81, RRID:AB_763582) and PE-CF594- conjugated anti-FoxP3 (1:50, Biolegend Cat# 320008, RRID:AB_492980) in Cytoperm at room temperature for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Ce3D™ Wash Buffer was removed completely and samples were placed in Ce3D™ Tissue Clearing Solution (12 hr, room temperature, with gentle shaking; 427704, Biolegend). Images were taken with Leica SP8 Confocal System (Leica Microsystems).
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol) were stained with αCD14-PE (BioLegend, San Diego, CA, USA) and αCD34-APC (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... pancreatic islets were harvested from four 12-week-old female NOD mice and labelled individually with anti-TCRβ (BioLegend, allophycocyanin, H57-597, 1:300), anti-CD4 (BioLegend ...