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Citations for BioLegend :
501 - 550 of 7909 citations for 7 3 2 3 5 dihydroxyphenyl 2 hydroxyethyl amino propyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione monohydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + .03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-chicken ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... anti-IFNγ antibody (2 ug/ml; clone MD-1, Biolegend), anti-IL-4 antibody (2 ug/ml ...
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bioRxiv - Immunology 2021Quote: ... anti-IFNγ antibody (2 ug/ml; clone MD-1, Biolegend), bexarotene (1 μM ...
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bioRxiv - Genomics 2022Quote: ... 1 ng/mL of recombinant IL-2 (BioLegend, Cat#575402) and 1 μg /mL of soluble anti-CD28 (Clone ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (PE/BV421, Biolegend, 1:50, clone JES6-5H4), PDCA-1 (Pacific Blue ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG1 (Biolegend; RMG1-1; at 2 μg/ml), antimouse IgG2b (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with 4’,6-diamidino-2-phenylindole (DAPI) (422801, BioLegend, San Diego, CA) for 2 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Myc (1:2, 9E10 Hybridoma culture supernatant, 1:1000 Biolegend); rabbit anti pRab10 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 secretion was evaluated with the IL-2 assay ELISA kit (Cat#431801, Biolegend). The absorbance value at 450 nm/570 nm was detected by the microplate reader ...
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bioRxiv - Microbiology 2019Quote: ... the splenocytes were incubated for 2 h with 5 μg/ml brefeldin A (Biolegend) for intracellular trapping of cytokines and then stained with Zombie Aqua (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Genomics 2022Quote: ... anti-human CD82-PE/Cyanine 7 monoclonal antibody (BioLegend, #342109, 1:500 dilution) and anti-human CD56-PE/Cyanine 7 monoclonal antibody (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... PE/Cyanine 7 anti-mouse CD31antibody (Biolegend, 102418, clone:390, 1:100 dilution), and PE/Cyanine 7 anti- mouse CD45 antibody (Biolegend ...
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were additionally incubated with 7-AAD Solution (1:100, BioLegend, 420404) for 5 minutes to detect dead cells and passed through cell strainer tubes (Falcon ...
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bioRxiv - Immunology 2024Quote: ... and only in the presence of 1 ng/mL IL-7 thereafter (BioLegend). Cultures were analyzed and passaged every 3 days.
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bioRxiv - Immunology 2019Quote: ... anti-CD38– APC-Cy7 (clone HB-7; BioLegend), and anti-CD20–FITC (clone 2H7 ...
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bioRxiv - Cell Biology 2021Quote: ... surface staining was performed using 7-AAD (Biolegend) and fluorochrome-tagged antibodies to CD45 and CD117 (both from Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD45 PE Cyanine 7 (BioLegend Cat# 304125, RRID:AB_10709440), CD42b APC Cyanine7 (BioLegend Cat# 303920 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD49a PE/Cy7 (clone TS2/7, Biolegend), anti-CD45 allophycocyanin (APC ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-AAD (Biolegend) or ZOMBIE NIR (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... anti-GL-7-PE-Cy7 (cat.no:144620,Biolegend),anti-IgD-BV786 (cat.no ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were stained with 7-AAD (Biolegend) for live/dead discrimination ...
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bioRxiv - Immunology 2019Quote: ... and CD56-Phycoerythrin Cyanine 7 (clone NCAM; BioLegend).
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bioRxiv - Microbiology 2019Quote: ... Cellular DNA was counterstained with 7-AAD (BioLegend) and the cells were analyzed on a BD LSR Fortessa instrument.
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bioRxiv - Immunology 2020Quote: ... 7-AAD viability staining solution was from Biolegend. MART-1 peptide (MART-126-35A27L ...
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bioRxiv - Systems Biology 2019Quote: ... trypsinized and stained with 7-AAD (BioLegend 420404) or Zoombie NIR (BioLegend 423105 ...