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Citations for BioLegend :
301 - 350 of 7689 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Cancer Biology 2023Quote: ... Hepatocytes were resuspended in 2 mL FACS buffer (2 mM EDTA, 0.5% BSA in PBS) and stained with Zombie Violet (1:500) (Biolegend #423113), TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 20 μg/mL 7-AAD solution (7-amino-actinomycin D, Biolegend, 650 nm) for 15 min at room temperature in dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptotic B16.F10 cells were stained with 7-Amino-actinomycin D (7-AAD, Biolegend) to determine viability ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... 2/3 of the cells were fixed with 750μL fixation buffer (BioLegend, #420801) for 30min ...
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bioRxiv - Immunology 2019Quote: ... CD169 (PE; 7-239; BioLegend), CD28 (PECF594 ...
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bioRxiv - Immunology 2020Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...
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bioRxiv - Immunology 2020Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8 (clone 53-6.7 ...
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bioRxiv - Immunology 2021Quote: ... anti-CD169 (Biolegend, 7-239) antibodies and acquired with an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... or 7-aminoactinomycin D (BioLegend) for all other experiments.
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bioRxiv - Immunology 2021Quote: ... anti-CD169 (Biolegend, 7-239) antibodies and acquired with an Attune NxT Flow Cytometer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... anti-GL7 (GL-7, Biolegend), anti-CD95 (Jo2 ...
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bioRxiv - Immunology 2020Quote: ... CD38 (Biolegend clone HB-7), IFNg (Biolegend clone 4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... Thy1.1 (clone OX-7, BioLegend), CD8α (clone 53-6.7 ...
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bioRxiv - Immunology 2023Quote: ... 7-AAD (BioLegend or BD) was used to discriminate dead CTV+ cells via flow cytometry ...
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bioRxiv - Cell Biology 2024Quote: ... Streptavidin PECy-7 (BioLegend, #405206), Ly6A/E (Sca-1 ...
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bioRxiv - Biophysics 2023Quote: ... Dye 7-AAD (Biolegend, USA) was used for dead cell exclusion from the analysis ...
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bioRxiv - Immunology 2023Quote: ... Viability dye 7-AAD (Biolegend) were added to samples before acquisition on a flow cytometer.
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bioRxiv - Immunology 2023Quote: ... CD38-FITC (HB-7, Biolegend), CD45RA-PerCP-Cy5.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... or 7-aminoactinomycin D (BioLegend). Cells were double sorted for purity.
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... - β1 (clone HMb1-1, 1:200) (Biolegend), were also employed ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 488 anti-CD54 antibody (2 μg/mL, ICAM-1, YN1/1.7.4, BioLegend), and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2021Quote: ... and Dectin-2 expression were examined by using rat anti-mouse Dectin-1 (Biolegend) followed by Alexa-594-conjugated goat anti-rat (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... media was changed to complete EGM-2 with 1 ng/mL TNFα (Biolegend 575206) and shear stress of 1 dyn/cm2 was incorporated using a microfluidic pump (Ibidi 10902 ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + .03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-chicken ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Genomics 2019Quote: ... cells were stained in batches of 1×107 cells for 30 min at 4°C (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Genomics 2021Quote: ... This was followed by 30min incubation at 4 °C with a Streptavidin-PE (Biolegend, 1:5000), anti c-kit-APC (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Cancer Biology 2020Quote: ... Dead cells were excluded using 7-aminoactinomycin D (7-AAD, eBioscience) and zombie violet (BioLegend). FACS analysis was conducted using a MACSQuant analyzer (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...
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bioRxiv - Developmental Biology 2021Quote: ... then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421) (BioLegend – 133911; 0.25 µg per million cells) and rat anti-mouse CD31 (labelled with AF647 ...
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bioRxiv - Immunology 2022Quote: A 3-plex SARS-CoV-2 Serological IgG Panel (BioLegend LEGENDplex, Cat. No. #741132) was used for measuring IgG titre of the 3 key COVID-19 coronavirus derived proteins ...