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Citations for BioLegend :
501 - 550 of 8397 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Immunology 2022Quote: ... Tim-3 was either neutralized by anti-human Tim-3 antibodies (10 µg/mL) (Biolegend) for 1 h prior to treatment or through knockdown via duplexed siRNA (Integrated DNA Technologies ...
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bioRxiv - Immunology 2019Quote: ... Sections were labeled overnight at 4°C with mouse anti-human CD1c-PE (L161, Biolegend) or mouse anti-human Clec9A-PE (8F9 ...
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bioRxiv - Microbiology 2020Quote: ... Tetramer labelled cells were washed and stained with anti-CD4 (2µg/ml, RM4-4; Biolegend), anti-CD3 (2µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... We mixed the beads with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone H5C6) and rotated for 45 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... We lysed RBCs by adding 4 mL of 1X RBC lysis buffer (Biolegend, catalog #420301) to the resuspended pellet ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated overnight at 4°C with BV421 anti-Foxp3 (Clone MF-14, Biolegend) washed ...
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bioRxiv - Immunology 2019Quote: Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (Biolegend 103101 rat anti-CD45 ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 Brilliant Violet 421 and anti-IFN-γ PE (Biolegend. San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... CD127-BV785 (A019D5) and CD137-PE (4-1BB) (purchased from BioLegend, San Diego, CA, USA), CXCR5 (CD185)-PE-eFluor 610 (MU5UBEE ...
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bioRxiv - Cell Biology 2022Quote: ... Pacific Blue Annexin V (640917) antibodies for 30 mins at 4°C (all from Biolegend). Cells were resuspended with either DAPI or Draq7 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Samples were then incubated (30 min, 4°C) with antibody cocktail to CD4-PercP5.5 (BioLegend), CD8-eFluor450 (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) cells were incubated with anti-PD-L1 blocking antibody (Ab; 4 μg/mL; BioLegend #29702 ...
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bioRxiv - Immunology 2023Quote: ... tissue sections were stained O/N 4°C with either anti-CD11c-biotin (N418, Biolegend), anti-CD63 (NVG-2 ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 20 min in 150 μl 4% paraformaldehyde (BioLegend; cat. no., 420801) at 4 °C in darkness before a 30-min permeabilisation step (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 45 min at 4°C with the following antibodies from BioLegend diluted in FACS buffer (PBS with 2% FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend) and 50 ng/ml rhGM-CSF (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ and IL-4 production were determined using ELISA kits (BioLegend, San Diego, CA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Genetics 2023Quote: ... Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, #PRB-435P), Anti-MAP2 antibody 1:1000 (Abcam ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and mouse anti-tubulin beta-3 (BioLegend, Cat# 801202, RRID:AB_10063408, 1:500). All primary antibodies were incubated overnight at 4°C ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... rat anti-mouse/human Galectin 3 (BioLegend Cat# 125402, RRID:AB_1134238, 1:100), rabbit anti-TAX1BP1 (Sigma-Aldrich Cat# HPA024432 ...
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in PBS and incubated for 30 min at 4 °C with rat FITC-conjugated mAb against CD11b (1:200, BioLegend, CA, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Immunology 2024Quote: ... Cells were harvested and stained for 30min at 4°C with the following antibodies: anti-CD25 BV785 (Biolegend, Cat: 356139, 1:50), anti-CD127 PE (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Cell Biology 2022Quote: ... or 2 μg ml−1 IL-6 neutralizing antibody (MP5-20F3, Biolegend) at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... APC-Fire750-CD32a/b (FUN-2, Biolegend, #303220), PE-PD-1 (3.13 ...
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bioRxiv - Cancer Biology 2022Quote: ... and TIM-3 (F38-2E2) (Biolegend) were used to evaluate T cell phenotype ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 3:20,000 anti-CD28 (BioLegend 302913), and 1:10,000 (30 IU/mL ...
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bioRxiv - Bioengineering 2021Quote: ... LAG-3 BV421 (Biolegend clone 11C3C65), PD-1 PE-Cy7 (Biolegend clone EH12.2H7).
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Galectin-3 (BioLegend, 126702), goat anti-Galectin-3 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... anti-GATA-3/BV421 (Biolegend, USA), anti-IL-4/BV510 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... anti-Tim-3 (F38-2E2, Biolegend), anti-IFN-γ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... OKT-3 stock (BioLegend Inc, USA) was diluted to 50mg/ul ...
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bioRxiv - Immunology 2020Quote: ... and CD11c (S-HCL-3, Biolegend) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant murine MMP-3 (#552704, BioLegend), recombinant murine c-RP (#50409-M08H ...
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bioRxiv - Cancer Biology 2021Quote: ... Galectin-3 (Biolegend, San Diego, USA), phospho-ERK1/2 ...