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Citations for BioLegend :
401 - 450 of 8149 citations for 6 METHYL 4 4 NITRO PHENYL 2 OXO 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The sorted fraction was sequentially stained for 20 min at 4 °C with the Ly51-PE antibody (1:800) (Biolegend) and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
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bioRxiv - Immunology 2021Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer or peptide probes preincubated with streptavidin (30 minutes at molar ratio of 1:4 at 25°C) conjugated to PE (405203, BioLegend), BV421 (405226 ...
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 4°C with KI67 primary antibody (cell signaling technology) diluted 1:400 or CD8a primary antibody (Biolegend) diluted 1:200 in Antibody diluent signal stain (Dako) ...
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bioRxiv - Immunology 2023Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer preincubated with streptavidin (30 min at molar ratio of 1:4 at 25°C) conjugated to phycoerythrin (405203, BioLegend) and/or BV421 (405226 ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells from the bone marrow were first stained with the following combination of biotinylated antibodies (lineage cocktail) for 30 minutes at 4 C: Gr-1 (RB6-8C5; Biolegend), Ter-119 (TER-119 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were fixed with ice-cold 4% PFA and stained with anti-Tubulin β3 (clone TUJ1, Biolegend 801201, 1:1,000) to visualize regenerating neurites ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml GM-CSF + 500 U/ml IL-4 (Biolegend, https://www.biolegend.com), respectively ...
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bioRxiv - Genomics 2021Quote: ... followed by incubation for 24 h at 4 °C with anti-Cas9 (BioLegend, 844301) primary antibody diluted in wash buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were then incubated overnight at 4 degrees with primary antibody-anti-Vimentin (Biolegend). Coverslips were washed and stained with secondary antibody ...
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bioRxiv - Genomics 2022Quote: ... at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend) and pooled equally as described previously18 ...
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bioRxiv - Immunology 2022Quote: ... mice received an intravenous injection of 3ug of anti-CD4 (RM4-4, Biolegend 116022) antibody and were euthanized 3 minutes after injection.
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bioRxiv - Immunology 2022Quote: Mice received an intravenous injection of 3ug anti-CD4-biotin (RM4-4, Biolegend 116010) antibody 3 minutes prior to euthanasia ...
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bioRxiv - Immunology 2022Quote: ... cells were re-stimulated for 4 hr with PMA/Ionomycin and Brefeldin A (Biolegend), according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... After 4 days of co-culture cells were collected stained for CD8-PECy5 (BioLegend) and assessed for cell proliferation with flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Cells were placed in 4 ml of 1X annexin binding buffer (Biolegend, cat#: 422201) with biotinylated annexin V (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured for 4 days prior to staining with anti-CD8-APC (SK1, BioLegend) and proliferation measured by flow cytometry ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked with TruStain FcX (α-mouse CD16/32; 10 min, 4°C; #101320, BioLegend) and stained (20 min ...
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bioRxiv - Immunology 2023Quote: ... Activation was measured by the following markers: CD137 APC (4:100; Biolegend Cat# 309810) and OX40 PE-Cy7 (2:100 ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with the anti-mouse IFNγ AN18 antibody (Biolegend) at 2 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Capture and biotin conjugated detection anti-mouse IL-4 antibodies were purchased from BioLegend. Anti-mouse IL-13 antibody pair were purchased from Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112, BioLegend) and anti-Gb1-4 (sc-378 ...
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bioRxiv - Immunology 2023Quote: ... tissue sections were stained O/N 4°C with either anti-CD11c (N418, Biolegend) and anti-CD63 (NVG-2 ...
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bioRxiv - Immunology 2024Quote: ... The PE-conjugated antibody for murine TLR-4 antibody (145404) was purchased from BioLegend. anti-TNFR1 (Armenian hamster IgG ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cell Biology 2019Quote: ... anti Centrin-2 (CETN2, Biolegend, 1:160), anti-LUZP1 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Immunology 2023Quote: ... 2/3 of the cells were fixed with 750μL fixation buffer (BioLegend, #420801) for 30min ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...