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Citations for BioLegend :
301 - 350 of 7702 citations for 6 Chloro 4 hydroxy 3 methoxycarbonyl 2H thieno 2 3 e 1 2 thiazine 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... - β1 (clone HMb1-1, 1:200) (Biolegend), were also employed ...
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bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
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bioRxiv - Immunology 2019Quote: ... in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Streptavidin M280 Dynabeads were conjugated to biotinylated HLA-C*05:01 refolded with P2-AV or P2-AW as described below.
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Cell Biology 2019Quote: ... with 2□mM EDTA for flow analysis or resuspended in PBS (pH 7.2) with 2□mM EDTA and 1□μg/ml DAPI (Biolegend) for sorting ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... and Alexa Fluor 647-anti mouse/human CD324 (E-cadherin) (clone DECAMA-1) was obtained from Biolegend.
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... and GATA-3-AP488 (Biolegend) was carried out using the same method but without stimulation.
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bioRxiv - Immunology 2020Quote: ... 3) CD62L APC-Cy7 (BioLegend), huCD45 PE (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... LAG-3-BV711 (11C3C65, Biolegend) and TIGIT-BV605 (A15153G ...
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bioRxiv - Microbiology 2020Quote: ... and human Hashtag 3 (Biolegend) at 4°C for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... LAG-3 (BioLegend, 125212, C9B7W), ST2 (eBioscience ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit-β-Tubulin 3 (BioLegend) and mouse-STEM101 (Takara Bio) ...
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bioRxiv - Immunology 2022Quote: ... anti-TIM-3-BV421 (Biolegend), and anti-human MR1-5-OP-RU Tetramer (NIH Tetramer Core Facility) ...
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bioRxiv - Immunology 2022Quote: ... anti-LAG-3-BV786 (Biolegend), anti-CD69-BUV563 (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... anti-LAG-3-BV786 (Biolegend), anti-CD69-APC (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... anti-LAG-3-BV786 (Biolegend), anti-CD25-BV650 (Biolegend) ...
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bioRxiv - Cell Biology 2023Quote: ... PDL1 (29E.2A.3, Biolegend), TCR (IP26 ...
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bioRxiv - Immunology 2023Quote: ... anti-CD5 (53.7-3, Biolegend), anti-CD103 (2E7 ...
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bioRxiv - Cancer Biology 2024Quote: ... KIR2DL2/3 (clone DX27, Biolegend), and KIR2DL1 (clone HP-MA4 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse TYRP1 (MEL-5) (1:500; 4°C overnight; BioLegend, 917801), rabbit MITF (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... βIII-tubulin (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and α-smooth muscle actin (αSMA ...
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bioRxiv - Immunology 2022Quote: ... 1 µg/ml anti-IL-4 (clone 11B11) (all from Biolegend), 20 ng/ml IL-6 ...
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bioRxiv - Immunology 2022Quote: ... 1 µg/ml anti-IL-4 (clone 11B11) (both from Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 4 % goat serum and 1:300-diluted TruStain FcX™ (Biolegend) for 1 h at RT in a humidified dark chamber ...
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bioRxiv - Immunology 2023Quote: ... PE anti-human CD137 (4-1BB (1:100 dilution, BioLegend, 309803). After staining ...
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bioRxiv - Immunology 2024Quote: ... CD4-APC (1:80; clone RM4-4; BioLegend Cat. No. 116013), CD4-BV421 (1:160 ...
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bioRxiv - Immunology 2024Quote: ... CD4-BV421 (1:160; clone RM4-4; BioLegend Cat. No. 116023), FoxP3-APC (1:20 ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... CD18 (clone CBR LFA-1/2, 366310), ITGB7 (clone FIB504, 121006), and CD68 (clone Y1/82A, 333814) were acquired from Biolegend.
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bioRxiv - Cancer Biology 2019Quote: ... or 1 million cells (analysis)No in FACS buffer (PBS with 2% FBS, 1X pen/strep and 1 mM EDTA) containing human TruStain FcX (Biolegend) and mouse TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...