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Citations for BioLegend :
451 - 500 of 7715 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Physiology 2022Quote: ... 647-conjugated CD324 (E-cadherin, 1:50, added during secondary antibody incubation, BioLegend); and Cytochrome P450 2E1 (Cyp2e1 ...
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bioRxiv - Cell Biology 2022Quote: ... I-A/I-E Alexa Fluor® 647 (MHCII) (rat, 1:100; Biolegend); mKi67 (rabbit ...
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bioRxiv - Immunology 2021Quote: ... and Pacific Blue anti-mI-A/I-E (M5/114.15.2) (1:400) (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Brilliant Violet 711 anti-mouse Ly-6A/E (Sca-1) (Biolegend, Cat. 108131), APC/Cy7 anti-mouse CD48 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... anti-Ly-6A/E (Sca-1)-Brilliant Violet 421 (BioLegend, clone D7, #108127), anti-CD48-Alexa Fluor 700 (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with 1 µM carboxyfluorescein succinimidyl ester (CFSE) dye (BioLegend, San Diego, CA, USA) for 15 minutes under standard cell culture conditions ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining cells were incubated with Biotin anti-mouse Ly-6A/E (Sca-1) antibody (Biolegend; Cat. No. 122504; 1:75) at 4°C for 20 minutes then 10 µl Streptavidin beads (1:30 ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC/Fire 750 conjugated anti E-Cadherin (dilution: 1:100; clone DECMA-1. All antibodies purchase from BioLegend (CA, USA). Samples were washed in 1mL 2% FBS and PBS and centrifuged at 2000rpm for 5mins ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD309 (VEGFR2) (Biolegend, clone 7D4-6; 1:50); anti-human CD56 (NCAM ...
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bioRxiv - Neuroscience 2021Quote: ... anti-Pax 6 (1:100 901301; BioLegend, San Diego, CA), anti-Tuj1 (801202 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ng/ml interleukin-6 (IL6, catalogue no. 570802; BioLegend), macrophage inflammatory protein (MIPα ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD63-160Gd (clone NVG-2, BioLegend), anti-mouse CD120b-166Er (clone TR75-32.4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...
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bioRxiv - Immunology 2020Quote: ... A647 anti-Bcl-2 (633510) (all from BioLegend); APC anti-CXCR4 (558644) ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138 (BV711, 281-2, AB_2562571, BioLegend #142519), anti-CD19 (BV785 ...
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bioRxiv - Immunology 2019Quote: ... 100U/mL IL-2 (Roche Diagnostics or Biolegend) was used throughout ...
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bioRxiv - Bioengineering 2021Quote: ... IL-2 PE-Cy7 (Biolegend clone MQ1-17H12), IFNγ APC (Biolegend clone B27) ...
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bioRxiv - Immunology 2020Quote: ... CD49d (α4-integrin; FITC, Biolegend, Clone: R1-2), CD88 (PE ...
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bioRxiv - Immunology 2022Quote: ... and 10 ng/mL IL-2 (589102, BioLegend) in RPMI-1640 complete medium for 24 hours before killing to differentiate into T cells ...
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bioRxiv - Immunology 2022Quote: ... BV785-labeled anti-CD138 (clone: 281-2; BioLegend), APC-labeled anti-IgD (clone ...
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bioRxiv - Immunology 2022Quote: ... APC-labeled anti-IgD (clone: IA6-2; BioLegend), PerCP-Cyanine5.5-labeled anti-CD38 (clone ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD32-PE-Cy7 (FUN-2, Biolegend). After ...
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bioRxiv - Immunology 2020Quote: ... 2 μl of Trustain FcR blocking reagent (BioLegend), 3 μl of anti-human CD45 antibody (clone HI100 ...
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bioRxiv - Bioengineering 2020Quote: ... Then a biotinylated anti-IL-2 Ab (BioLegend) at a concentration of 0.25 μg/ml was added and after washing ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated SARS-CoV-2-S1 Protein (Biolegend 793804) Anti-Biotin (409003) ...
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bioRxiv - Immunology 2020Quote: ... and rIL-2 (10 U/ml; Biolegend, #575402), with or without 2.5 μM mCRAMP ...
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bioRxiv - Immunology 2020Quote: ... p-Erk1/2 (4B11B69) were purchased from BioLegend. Antibodies against NFAT1 (D43B1) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μL anti-Rpb3 antibody (Biolegend, catalog #665003) was conjugated to Dynabeads M-280 sheep anti-mouse IgG beads (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and 20 ng/ml IL-2 (Biolegend; 575406) as previously described (38) ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-mouse CD14 (clone Sa14-2; Biolegend), rabbit monoclonal Alexa 647 anti-phospho-p65(Ser536 ...
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bioRxiv - Immunology 2021Quote: ... OX40 PE-Cy7 (2:100; Biolegend Cat#350012), and CD69 PE (10:100 ...
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bioRxiv - Immunology 2021Quote: ... and anti-IL-2-APC (JES6-5H4, BioLegend) on ice for 1 hr or overnight ...