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Citations for BioLegend :
451 - 500 of 506 citations for 6 Chloro 7 methylisatin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 6-18 (Tau 12, 1:1,000 dilution, ref: 806501, Biolegend), 159–163 (Tau HT7 ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... slides were incubated for 1.5 hrs at RT with a cocktail of secondary fluorescence-conjugated antibody Alexa488 and DAPI (4’,6-diaidino-2-phenylindole, dilactate; BioLegend) to visualize nuclei ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 μM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... The sections were then rinsed in a washing solution twice before nuclei were stained with 4’,6-diamidino-2-phenylidole (DAPI; BioLegend). The sections were rinsed with washing solution one final time and then mounted on slides for examination under an Olympus FV1000 laser-scanning 4-channel confocal microscope (Olympus).
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...