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Citations for BioLegend :
501 - 550 of 7102 citations for 6 Bromo 3 phenyl imidazo 1 2 a pyridine 2 carboxylic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
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bioRxiv - Immunology 2024Quote: ... plates were coated per well with 100 µL of anti-IgE capture antibody (2 µg/mL in PBS, clone RME-1, BioLegend) overnight at 4⁰ C ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Immunology 2021Quote: ... PD-1 (29F.1A12) and Bcl-6 (K112-91) from BioLegend and BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... and Alexa Fluor 647-conjugated BCL-2 (BioLegend Cat# 633510, RRID:AB_2274702). PE-Cy7-conjugated Streptavidin was used to detect biotinylated CD49d (9C10).
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bioRxiv - Immunology 2021Quote: ... anti-mouse mouse MHC H-2 hashtag antibodies (Biolegend TotalSeq™) were used to label separate FACS-purified subsets which were washed ...
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bioRxiv - Immunology 2021Quote: ... The antibody against Bcl-2 (BCL/10C4) was purchased from Biolegend. The antibodies against TCF1 (C63D9 ...
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bioRxiv - Bioengineering 2021Quote: ... and APC anti-human CD46 (clone TRA-2-10, Biolegend 352405). Antibody staining was performed for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with cold 2 ml staining buffer (BioLegend Inc, catalog # 420201) and resuspended in cold Annexin V binding buffer at 1×106 cells per ml ...
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bioRxiv - Immunology 2022Quote: ... PMN were stained with 2 μl of Zombie Aqua dye (BioLegend) diluted 1:100 for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... AM were imaged PE-Siglec F (2 µg, clone S17007L; BioLegend) administered intravenously 30 mins after engraftment ...
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bioRxiv - Immunology 2022Quote: ... APC-anti human 2B4/CD244 (2-69; BioLegend, San Diego, CA); or with the isotype control APC mouse IgG1 (MOPC-21 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138-PE (281-2) and PE/Cy7- Streptavidin from Biolegend, and anti-FCRL5-Alexa488 and anti-FCRL5-APC (Polyclonal ...
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bioRxiv - Bioengineering 2020Quote: All reagents for detection of IL-2 were purchased from Biolegend. Supernatants were collected after 16 hours of coculture of 5MCAR 58α−β− cells and M12 target cells ...
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bioRxiv - Immunology 2020Quote: ... CD19 Pacific Blue (clone H1B19, BioLegend cat#302224, 2:100 dilution); CD3 AF700 (clone OKT3 ...
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bioRxiv - Immunology 2020Quote: ... CD4 PerCP-Cy5.5 (clone OKT4, BioLegend, cat# 317428, 2:100 dilution); CD127 AF488 (clone A019D5 ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 2 μg/ml of anti-CD28 (clone CD28.2, Biolegend) and with 10 ng/ml of recombinant IL-2 (Peprotech ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μl Zombie Aqua dye (423101, BioLegend, San Diego, CA, USA), and 5 μl DNase I and subjected to flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 antibody (2 ug/mL, Clone: CD28.2, Biolegend) in the culture medium containing 1×Brefeldin A (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... cRPMI media was supplemented with 50 ng / mL IL-2 (Biolegend), 50 ng / mL IL-15 (Biolegend).
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bioRxiv - Immunology 2023Quote: ... together with CD137 APC antibody (2:100; Biolegend, San Diego, CA). Cells were then stained on their surface for 30 min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of biotinylated anti-mouse IL-2 (BioLegend), and then streptavidin-horseradish peroxidase conjugate (SA-HRP ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with CD206 Ab AF647 (clone 15–2, BioLegend) and dead cell marker Zombie Violet (BioLegend) ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine5 anti-mouse IL-2 antibody (BioLegend, catalog no. 503824), PE/Cyanine7 anti-mouse IL-4 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and stained with CD63 (NVG-2) and CD206 (C068C2) from BioLegend and pro-IL-1b (NJTEN3) ...
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bioRxiv - Bioengineering 2023Quote: ... and 500 IU/ml of recombinant human IL-2 (Biolegend, 589104). Stimulated T cells were co-transduced via spinoculation at 931xg ...
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bioRxiv - Bioengineering 2023Quote: All antibodies described in Supp Table 2 were purchased from Biolegend
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bioRxiv - Immunology 2023Quote: ... naïve or effector CFSE-labelled OT-I (2 µM CFSE, BioLegend) were added to coverslips in complete RPMI media for 30 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... as well as 2 µL of Monensin (Biolegend, San Diego, CA). Cells were cultured for 6 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and 2 μl PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532). Additionally ...
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bioRxiv - Cancer Biology 2024Quote: ... Media were supplemented with 2 ng/ml GM-CSF (BioLegend 572902) for OCI-AML5 ...
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bioRxiv - Immunology 2024Quote: ... was purchased from Bioxcell and recombinant mouse IL-2 (carrier free, cat # 575408 from Biolegend). IL-2/anti-IL-2 complexes were prepared by mixing both reagents ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Bioengineering 2020Quote: ... Jurkat cells were resuspended in 5 µM carboxyfluorescein diacetate succinimidyl ester (CFSE, Biolegend) in PBS for 20 min at room temperature in order to identify Jurkats specifically during cell sorting ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with FACS buffer (PBS+ 2% (v/v) FBS with 1mM EDTA) and incubated with a 1:500 dilution of TruStain FCX (Biolegend, 101320) for 5 minutes ...