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Citations for BioLegend :
251 - 300 of 369 citations for 6 Amino m ethylphenol hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and IL-4 and IL-6 using a LEGENDplex™ mouse inflammation cytokine assay (Biolegend). Collected blood was incubated at room temperature for 1 hr and centrifuged for 7 min at 12,000 g at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE anti-mouse H-2Kb/H2Db antibody (Clone 28-8-6; Biolegend; Cat #114607) on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2023Quote: ... macrophages were challenged with CFSE (5-[and 6]-Carboxyfluorescein diacetate succinimidyl ester Kit, BioLegend,423801)-labeled apoptotic cells at a 4:1 (AC/Macrophage ...
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bioRxiv - Immunology 2024Quote: WT C57Bl/6 mice were injected intraperitoneally with 250 ng recombinant mouse IL-33 (Biolegend) in 100 µl PBS for three consecutive days ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Bioengineering 2022Quote: ... Cytokine-primed BMDMs were cultured in RPMI growth media + 20 ng/mL M-CSF + 20 ng/mL IFNγ (BioLegend 575302) or 20 ng/mL IL-4 (BioLegend 574302 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng/mL granulocyte macrophage colony stimulating factor (GM–CSF, for M1 polarization) or 50 ng/mL macrophage colony-stimulating factor (M–CSF, for M2 polarization) (BioLegend, San Diego ...
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bioRxiv - Immunology 2023Quote: ... washed FACS buffer and stained with a 1:100 dilution of anti-mF4/80 or anti-m/hCD11b antibody (Biolegend) FACS buffer for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... using a LEGEND MAX™ Human IL-6 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) Kit (BioLegend) with pre-coated plates ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with 4’,6-diamidino-2-phenylindole (DAPI) (422801, BioLegend, San Diego, CA) for 2 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... All three groups were co-treated with IL-6 (10 ng/mL, BioLegend cat no. 575706). We included one control group that was treated with DMSO ...
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bioRxiv - Immunology 2023Quote: ... in the presence of Th17 polarizing cocktails containing recombinant mouse IL-6 (10 ng/ml, Biolegend), mouse IL-1β (10 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Peritoneal lavage cells were isolated and stimulated overnight at 37 °C with 50 ng/ml m-IL-1β (Biolegend, Cat# 575002) plus m-IL-23 (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Immunology 2023Quote: ... MΦs were generated by incubating bone marrow cells at 37°C with 40 ng/ml M-CSF (Biolegend, San Diego, CA) in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Immunology 2021Quote: ... C57BL/6 or Rag2-/- mice were treated intranasally (i.n.) with 100 ng of recombinant IL-33 (Biolegend) each day for 5 consecutive days ...
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bioRxiv - Immunology 2021Quote: ... and cells were instead stained with 4′,6-diamidino-2-phenylindole (DAPI; BioLegend, San Diego, CA, USA) and gated for live (DAPI- ...
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bioRxiv - Cancer Biology 2020Quote: ... LPS stimulation was performed in the presence of blocking anti-IL-6 antibody or control IgG (Biolegend), at a concentration of 1ug/mL.
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bioRxiv - Microbiology 2019Quote: ... IL-1β and IL-6 using the LEGENDplex multi-analyte flow assay kit (Biolegend, San Diego, CA) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Samples were stained with 3 μM of (4’,6-Diamidino-2-Phenylindole, Dilactate (DAPI, Biolegend Cat# 422801)) ...
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bioRxiv - Immunology 2022Quote: ... All samples were also stained with a unique anti-mouse TotalSeq-A Hashtag antibody (1-6; BioLegend) to allow up to 6 populations to be multiplexed in a single lane on a 10X Genomics Chromium Next GEM Chip96 ...
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Supernatants were collected and assessed for TNF-α and IL-6 using deluxe ELISA kits from Biolegend. 10mg of perfused liver tissue was rinsed with cold PBS ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with inhibitors for 6 hours and then stained with Zombie Aqua (BioLegend, Cat. # 423101) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mononuclear cells were collected and washed with complete medium and 104 cells per well in 96 wells plate were plated in the presence of 40ng/ml M-CSF(Biolegend; cat#576402) and 60ng/ml RANKL (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... anti-CD4 [OKT4], anti-CD8a [RPA-T8], anti-CD25 [M-A251], anti-CD45RA [HI100], and anti-CD127 [A019D5], all from BioLegend, San Diego, CA). Pilot experiments comparing FACS sorting and magnetic bead separation showed similar results with respect to cell purity ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... The levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected using ELISA kits (Biolegend) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... HTO staining was carried out with 0.5μg of β2M and CD298 antibody-conjugated oligonucleotides (Total-seq B Hastags 4, 6 and 8; BioLegend) with ∼500,000-750,000 isolated single cells ...
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bioRxiv - Immunology 2020Quote: ... mouse TNF-α (430904) and IL-6 (431304) enzyme-linked immunosorbent assay (ELISA) kits were purchased from BioLegend; Griess Reagent System (G2930 ...
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bioRxiv - Bioengineering 2021Quote: ... FITC anti-mouse H-2Kb/H-2Db Antibody (Clone 26-8-6) were from Biolegend (San Diego, CA). Primary antibodies of STING/TM173 (D2P2F) ...
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bioRxiv - Neuroscience 2023Quote: ... centrifugated (500g) and IL-6 secretion analyzed using a commercial ELISA kit following the manufacturer’s instructions (Biolegend, #431304).
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bioRxiv - Immunology 2022Quote: ... and two hashtag antibodies were built by combining the antibodies MHC I-Biotin (Biolegend, clone 28-8-6) and CD45-Biotin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... CD45-labelled cells were mixed (maximum 6 samples together) and stained with APC-conjugated FCRL5 antibodies (Biolegend #340305)(for panel #2) ...
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bioRxiv - Immunology 2023Quote: ... injections of a TH17 priming cocktail (n=8; 0.5μg rmIL-6 [575704, BioLegend, USA] and 1μg rmTGF-β1 [763104, BioLegend, USA]) or rest (n=8 ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was collected after 24 h to measure IL-6 and TNF-α levels using ELISA (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Immunology 2019Quote: ... Plasma IL-6 levels were determined by sandwich enzyme-linked immunosorbent assay (ELISA) (Biolegend ELISA MAX Deluxe, London UK). Values below the blank were reported at the limit of detection for statistical purposes ...