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Citations for BioLegend :
51 - 100 of 6996 citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... cells were incubated with antibodies to F4/80 (BM-8, Biolegend), CD11c (HL3 ...
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bioRxiv - Immunology 2021Quote: ... injection of 8 μg/mouse of recombinant interferon-α from Biolegend (752806) was administered ...
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bioRxiv - Biochemistry 2021Quote: ... The assay was performed using human IL-8 ELISA Max Deluxe (BioLegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... 100ng/ml of recombinant human (rh) IL-8 (Biolegend, San Diego, CA) were added to the apical side of the enteroid monolayers (76) ...
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bioRxiv - Immunology 2023Quote: ... IgM and IgD dosage by LEGENDplex (8 plex immunoglobulin isotyping panel, Biolegend) according to the manufacturer procedure ...
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bioRxiv - Bioengineering 2023Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; BioLegend) was diluted 1:10,000 from a 5 mg mL-1 stock solution in deionized water ...
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bioRxiv - Systems Biology 2022Quote: ... Each tube was made up to 100μL with cell staining buffer and TotalSeq Hashtag (HTO 1-8) reagents (BioLegend) were added to each sample for 20min on ice ...
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bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
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bioRxiv - Immunology 2022Quote: ... pooled 8:2 CD45+:CD45-cells were resuspended in Cell Staining Buffer (BioLegend) and stained with a pool of 137 TotalSeq-C antibodies (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and 8 clones that were negative by surface staining with W6/32 (BioLegend) grown out ...
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bioRxiv - Immunology 2023Quote: ... Pooled 8:2 CD45+:CD45-cells were resuspended in Cell Staining Buffer (BioLegend) and stained with a pool of 137 TotalSeq-C antibodies (Table ...
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bioRxiv - Microbiology 2024Quote: ... 1L-8 and IL-15 were quantified using individual ELISA kits from (Biolegend), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... glass slides or 8 well chambers were coated with either ICAM-1 alone or anti-CD3 (clone Hit3a from Biolegend)/anti-CD28 (clone CD28.2 from Biolegend)/ICAM-1 (R&D systems ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Zoology 2020Quote: ... IL-8 and IL-12 were gotten from Biolegend (Camino Santa Fe, CA, USA). BCA protein quantitative kit and SYBR Green PCR kit were purchased from Smer Fell Science and Technology Co. ...
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bioRxiv - Immunology 2022Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: μ-side 8-well Ibidi chambers were coated with an anti-CD31 antibody (102502, BioLegend) for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Four μg of Tau12 (N-terminal [epitope = amino acids 6-18], BioLegend), HT7 (mid-region [epitope = amino acids 159-163] ...
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bioRxiv - Immunology 2019Quote: ... or DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate; Biolegend). Intracellular staining was carried out using Human FoxP3 Buffer (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... 3 and 6 were stained with Calcein-AM (BIOLEGEND, 425201) and PI (SIGMA ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (4-6; BioLegend). The enriched samples were then stained with APC-labeled Streptavidin (BioLegend ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea pig anti-Cytokeratin 8+18 antibody and FITC rat anti-mouse/human CD45R/B220 (BioLegend) were used for fluorescence immuno-histochemistry ...
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bioRxiv - Immunology 2020Quote: ... 8°C) and incubated for 10 min at 37°C and Zombie NIR Viability dye (Biolegend) was added to each well ...
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bioRxiv - Immunology 2022Quote: ... and stained again with biotinylated anti-mouse/human TLR7/8 mAb and PE streptavidin (Biolegend, USA). To detect intracellular mouse IL-6 in splenocytes stimulated with various ligands in the presence of Brefeldin A (10μg/ml) ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Cell Biology 2021Quote: ... and collected data analysed and presented by FlowJo V.10.5.0 (FlowJo LLC, OR, USA, www.FlowJo.com) 8-Peaks Rainbow Calibration Particles (Biolegend #422903) was used for routine alignment and performance verification of the flow cytometry ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: Antibodies and clones reactive against the following proteins were used: CD2 (TS1/8, BV421 or Pacific Blue, Biolegend), CD3 (UCHT1 ...
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bioRxiv - Neuroscience 2022Quote: ... room temperature incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421TM Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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bioRxiv - Neuroscience 2019Quote: ... RT incubation in 2X SSC containing 8 µg/µl Brilliant Violet 421™ Streptavidin (BioLegend, Cat. No. 405225) and 0.05% Tween 20 ...
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bioRxiv - Genetics 2022Quote: ... cKit-BV510 (104D2) and NKp44-Alexa Fluor (AF)647 (P44-8) all purchased from Biolegend (San Diego, CA), CRTH2-PE (301109 ...
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bioRxiv - Microbiology 2021Quote: ... Sections were blocked with 10% sheep serum and 1% BSA in TBS for 30 min then incubated at 4°C overnight with 1 μg ml−1 rabbit polyclonal anti-tubulin β-3 IgG (Biolegend) and 1% BSA in TBS ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
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bioRxiv - Microbiology 2019Quote: ... cell free supernatant was collected after 24 hours and IL-8 was quantified by ELISA according to manufacturer’s instructions (BioLegend).
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bioRxiv - Bioengineering 2022Quote: ... the streptavidin modified nanovial solution was then mixed with a solution containing 8 μg/mL anti-human CD45 (Biolegend) at equal volume ...
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bioRxiv - Cancer Biology 2023Quote: ... for 8 h at 37 °C followed by staining with a fixation/permeabilization buffer according to the manufacturer’s protocol (BioLegend). After washing ...
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bioRxiv - Immunology 2021Quote: ... Bcl-6 FITC (Biolegend 358513, 1:100), and Glut-1 AlexaFluor 647 (Abcam ab115730 ...
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Immunology 2022Quote: The presence of IL-17A in T-cell culture supernatants and of IL-6 and IL-8 in fibroblast culture supernatants was quantified by ELISA according to manufacturer instructions (deluxe or standard kits, respectively, all BioLegend). Plates were read at 450 nm using a Spark 10M (Tecan) ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Pathology 2019Quote: ... IL-9 and IL-6 determinations (Human IL-4, IL-5, IL-9 and IL-6 MAX, Biolegend, San Diego, CA 92121 USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Machine calibration was performed by flow cytometry facility staff before each sort using Sphero™ 8-peak rainbow calibration beads (BioLegend) to achieve optimal stream alignment ...
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bioRxiv - Neuroscience 2021Quote: ... Measurements of cytokine secretion were made 8 and 24 hrs after activation in the presence of BSA (0.4 μM) using Max deluxe (Biolegend, CA, USA) ELISA kits [24] ...