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Citations for BioLegend :
151 - 200 of 2419 citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Bioengineering 2021Quote: ... or 25 ng/ml Interleukin-4 (IL-4) (Biolegend) were used to stimulate M1 or M2 polarization ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 5 ng/ml IL-5 (Biolegend; 581504), and 20 ng/ml IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... streptavidin cyanine 5 (5 μg/mL, BioLegend) for 1 h ...
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bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Genomics 2019Quote: ... Measurement of BCL-6 expression in targeted Jurkat lines was assessed by flow cytometry using anti-human APC-BCL-6 (Biolegend) after treatment with human recombinant IFNg (5 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... human Interlukin-6 (IL-6) (100 ng/ml) and human IL-6Rα (100 ng/ml) (BioLegend, San Diego, CA, USA).
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bioRxiv - Immunology 2020Quote: ... cytokine staining mixture (containing anti-IFN-γ, anti-IL-2, anti-IL-4, anti-IL-10 and anti-TNF-α antibodies, Biolegend) was added to each well ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... HLA-DR-PerCP-Cy5.5 (clone G46-6, Biolegend), CCR5-PE (clone 3A9 ...
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bioRxiv - Cell Biology 2022Quote: ... and IL-6 (MP5-20F3, Biolegend; 1:100) antibody ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and IL-6 (BioLegend Inc., San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: ... prior to BCL-6 AF647 (IG191E/A8, Biolegend) and Ki-67 BUV395 (B56 ...
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bioRxiv - Immunology 2022Quote: ... and APC anti-mouse IL-6 (Biolegend 503810). Data was acquired on a MACSQuant10 (Miltenyi ...
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bioRxiv - Genomics 2022Quote: ... CD151 (clone 50-6, BioLegend 350405, RRID AB_10661726), CD29 (clone TS2/16 ...
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bioRxiv - Bioengineering 2023Quote: ... CD66b-BV421 (1:50, clone 6/40c, Biolegend), CD127-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... Th17: IL-6 (20 ng/ml, BioLegend, 575702), TGF-β1 (1 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... IL-6-PE or APC (both from Biolegend) and IL-13-PE or eFluor 450 (eBioscience) ...
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: ... The levels of murine IL-6 and TNF-α and human IL-6 were measured using ELISA MAX™ kits (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: Antibody levels in the supernatants from B cells that had been stimulated for 6 days were measured using the LEGENDplex™ Human Immunoglobulin Isotyping Panel (6-plex) (Biolegend), a bead-base immunoassay ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... All iPSCs were stained in PBS with 5% FBS overnight at 4°C with the following antibodies and dilutions: Alexa Fluor® 647 anti-tubulin α (1:1000, Biolegend, #627908, clone 10D8), and Alexa Fluor ® 488 anti-Oct-4A (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Immunology 2022Quote: ... and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Figure 6 A: CD326-BV785 (1:100, BioLegend, 9C4), CD235a-FITC (1:100,eBioscience ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... and stained with FITC-labelled anti-IL-6 (BioLegend). Acquisition was performed on a FACSVerse flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or 6 μg/mL anti-ICOS (clone C398.4A, Biolegend) in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... clone HI9a (6 µg/mL, BioLegend, San Diego, USA); anti-CD63–APC ...
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bioRxiv - Immunology 2020Quote: ... anti-MHC class I (clone 28-8-6, BioLegend), anti-β-catenin (clone D2U8Y ...
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bioRxiv - Cell Biology 2021Quote: ... and CD309/Kdr/Flk-1 (7D4-6)-APC (BioLegend). 4’ ...
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bioRxiv - Immunology 2022Quote: ... or α-IL-6 (BioLegend, 1:100, MP5-20F3) antibodies for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: IL-6-APC (BioLegend, 504508, MP5-20F3, 1 μL)
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bioRxiv - Bioengineering 2024Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse TotalSeq-A Hashtag antibody (1-6; BioLegend), PE-labeled Siglec F (S17007L ...
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bioRxiv - Immunology 2024Quote: ... and cytokine stains with IL-6 (MP5-20F3; Biolegend), IL-10 (JES5-16E3 ...