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151 - 200 of 779 citations for 5 Nitrofluorescein since 2019

• Microfluidics-free single-cell genomics with templated emulsification

  Iain C. Clark, et al., bioRxiv - Genomics 2022

  Quote: ... 5 μL Trustain FcX block (BioLegend, 422301) was added per million cells and gently mixed 10 times with a wide-bore pipette tip ...
• Cellular sources and targets of type I interferons that drive susceptibility to tuberculosis

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2022

  Quote: ... APC-labeled CD64 (X54-5/7.1, BioLegend), BV480-labeled B220 (RA3-6B2 ...
• Autophagy functions in lung macrophages and dendritic cells to suppress T_H17 responses and neutrophil accumulation during allergic airway inflammation

  Neha Dubey, et al., bioRxiv - Immunology 2023

  Quote: ... CD64-PerCP-eFluor710 (Biolegend, X54-5/7.1), MerTK-PE-Cy7 (Biolegend ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and anti-human IL-5 (TRFK5, Biolegend)neutralizing antibodies (10 ug/ml each ...
• A PD-1-ST2 axis controls Th2 effector function in tissue via a metabolic checkpoint

  Graham A. Heieis, et al., bioRxiv - Immunology 2023

  Quote: ... IL-5 (TRFK5) conjugated to PE (BioLegend); IL-13 (eBio13A ...
• Cellular iron governs the host response to malaria

  Sarah K. Wideman, et al., bioRxiv - Immunology 2023

  Quote: ... 10 ng/mL IL-5 (581502, BioLegend) and 50 μM 2-Mercaptoethanol ...
• Synergistic activity of IL-2 mutein with tolerogenic ImmTOR nanoparticles leads to massive expansion of antigen-specific Tregs and protection against autoimmune disease

  Takashi Kei Kishimoto, et al., bioRxiv - Immunology 2023

  Quote: ... CD4-PerCP-Cy5.5 (BioLegend, clone RM4-5), CD8a-BV510 (BioLegend ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-mCD64 (X54-5/7.1, BioLegend, 139304), anti- mCD71 (RI7217 ...
• Soluble CTLA-4 mainly produced by Treg cells inhibits type 1 inflammation without hindering type 2 immunity to allow for inflammation resolution

  Motonao Osaki, Shimon Sakaguchi, bioRxiv - Immunology 2023

  Quote: ... anti-m/hIL-5 (TRFK5, BioLegend, 504304), anti-mIL-17A (TC11-18H10.1 ...
• Zfp318 controls the recallability of memory B cells

  Yifeng Wang, et al., bioRxiv - Immunology 2023

  Quote: ... MitoSpy™ NIR DiIC1(5) from Biolegend; FITC anti-IgG2c from Bethyl ...
• Reduced SARS-CoV-2 mRNA vaccine immunogenicity and protection in mice with diet-induced obesity and insulin resistance

  Timothy R. O’Meara, et al., bioRxiv - Immunology 2022

  Quote: ... and brefeldin A (5 μg/ml, BioLegend). After a 6-hour stimulation ...
• Homeostatic activation of Aryl Hydrocarbon Receptor by dietary ligands dampens cutaneous allergic responses by controlling Langerhans cells migration

  Adeline Cros, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD4 BV650 (Biolegend, clone RM4-5), anti-Ly6C AF700 (BioLegend ...
• A multiadjuvant polysaccharide-amino acid-lipid (PAL) subunit nanovaccine generates robust systemic and lung-specific mucosal immune responses against SARS-CoV-2 in mice

  Bhawana Pandey, et al., bioRxiv - Bioengineering 2023

  Quote: ... and 5 μg/mL Brefeldin A (Biolegend). After incubation for 6 h ...
• Validation of ligand tetramers for the detection of antigen-specific lymphocytes

  Kristin S. Fitzpatrick, et al., bioRxiv - Immunology 2022

  Quote: ... anti-mouse CD4 BV711 (RM4-5, BioLegend), anti-mouse CD4 BV421 (GK1.5 ...
• Diet composition drives tissue-specific intensity of murine enteric infections

  Helene Israelsen, et al., bioRxiv - Microbiology 2023

  Quote: ... 5 µg of IL-25 (#587306; BioLegend), or sterile PBS as a vehicle control ...
• Immunosuppression is a conserved driver of tuberculosis susceptibility

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2023

  Quote: ... TotalSeq-A-labeled CD4 (RM4-5, BioLegend), TotalSeq-A-labeled ST2 (DIH9 ...
• Phagocytosis in macrophages is regulated by the ATP-binding cassette family gene ABCF1

  Hitesh Arora, et al., bioRxiv - Immunology 2023

  Quote: Anti CD64 antibody (BioLegend; X54-5/7.1)
• Temporal genomic analysis of melanoma rejection identifies regulators of tumor immune evasion

  Sapir Cohen Shvefel, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and labeled with CFSE (5 M, Biolegend). 1 × 105 cells in 100 μL per well were plated in a 96-well plate pre-coated with anti-mouse CD3ε (1 μg/mL ...
• Immunosuppression is a conserved driver of tuberculosis susceptibility

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2023

  Quote: ... APC-labeled CD64 (X54-5/7.1, BioLegend), BUV563-labeled Ly6G (1A8 ...
• Single-Cell Profiling Reveals a Naive-Memory Relationship between CD56^bright and Adaptive Human Natural Killer Cells

  M. Kazim Panjwani, et al., bioRxiv - Immunology 2023

  Quote: ... NKp46 (Biolegend, Clone 9E2: PerCP-Cy5-5), NKp30 (Miltenyi ...
• Immunosuppression is a conserved driver of tuberculosis susceptibility

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2023

  Quote: ... TotalSeq-A-labeled CD4 (RM4-5, BioLegend), TotalSeq-A-labeled ST2 (DIH9 ...
• Immunosuppression is a conserved driver of tuberculosis susceptibility

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2023

  Quote: ... APC-labeled CD64 (X54-5/7.1, BioLegend), and TotalSeq-A-labeled Ly6G (1A8 ...
• Type I IFN signaling in the absence of IRGM1 promotes M. tuberculosis replication in immune cells by suppressing T cell responses

  Sumanta K. Naik, et al., bioRxiv - Immunology 2023

  Quote: ... CD4-BV570 (BioLegend#100541;clone RM4-5), Ly-6C-BV605 (BioLegend#128036;clone HK1.4) ...
• Lung influenza virus specific memory CD4 T cell location and optimal cytokine production are dependent on interactions with lung antigen-presenting cells

  Kerrie E Hargrave, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD64-PE (BioLegend, X54-5/7.1), anti-CD11c-Alexa-594 (BioLegend ...
• Inheritance of old mitochondria controls early CD8⁺ T cell fate commitment and is regulated by autophagy

  Mariana Borsa, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-CD28 (5 μg/ml) (37.51, BioLegend) and recombinant human or murine Fc-ICAM-1 (10 μg/ml ...
• Activated STING in the thymus alters T cell development and selection leading to autoimmunity

  Zimu Deng, et al., bioRxiv - Immunology 2024

  Quote: ... and 5 µg/ml Brefeldin A (Biolegend) at 37°C for four hours.
• Inflammation induced T_h17 cells synergize with the inflammation-trained microbiota to mediate host-resiliency against intestinal injury

  JL Golob, et al., bioRxiv - Immunology 2024

  Quote: ... CD4 BV 650 (BioLegend, clone RM4-5), CD69 PE-Cy7 (BioLegend ...
• Integrin inactivation slows down neutrophils congesting the pre-metastatic lung in a model of breast cancer

  Frédéric Fercoq, et al., bioRxiv - Immunology 2024

  Quote: ... CD4-PE/Cy7 (clone RM4-5, Biolegend).
• Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques

  Timothy N. Hoang, et al., bioRxiv - Immunology 2020

  Quote: ... anti-IL-2 BV650 (clone MQ1-17H12; 5 μL; cat# 500334) and anti-IFNγ PE/Dazzle 594 (clone B27; 5 μL; cat# 506530) both from Biolegend; anti-IL-17a Alexa Fluor 488 (clone eBio64DEC17 ...
• Leishmania amazonensis infection induces PD-L1 expression on dendritic cells in an mTor-dependent manner and impairs Th1 responses in vitro and in vivo

  Herbert L. de Matos Guedes, et al., bioRxiv - Immunology 2022

  Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
• Modeling diverse genetic subtypes of lung adenocarcinoma with a next-generation alveolar type 2 organoid platform

  Santiago Naranjo, et al., bioRxiv - Cancer Biology 2021

  Quote: ... rabbit anti-Keratin 5 (1:5000, Biolegend, 905501), anti-TTF1 (1:500 ...
• Multi-modal pooled Perturb-CITE-Seq screens in patient models define novel mechanisms of cancer immune evasion

  Chris J. Frangieh, et al., bioRxiv - Genomics 2020

  Quote: ... and 5 μL TruStain FcX blocking antibodies (Biolegend) were added and incubated for 10 minutes on ice ...
• Specialized subsets of innate-like T cells and dendritic cells protect from lethal pneumococcal infection in the lung

  Mallory Paynich Murray, et al., bioRxiv - Immunology 2021

  Quote: ... anti-CD64-PE Cy7 (X54-5/7.1, BioLegend); anti-CD86 (GL-1 ...
• Molecular and functional diversity of distinct subpopulations of extracellular vesicles from stressed pancreatic beta cells: implications for autoimmunity

  Khem Raj Giri, et al., bioRxiv - Immunology 2020

  Quote: ... and 5 ng/mL IL4 (BioLegend, London, UK) and 2 × 106 cells were cultured in 10mL of medium per 100 mm Petri dish for ten days ...
• Immunogenicity of SARS-CoV-2 trimetric spike protein associated to Poly(I:C) plus Alum

  Júlio Souza dos-Santos, et al., bioRxiv - Immunology 2022

  Quote: ... blocked with 5 µL/well Human FcX (BioLegend) for 15 min ...
• CXCR2 inhibition enables NASH-HCC immunotherapy

  Jack Leslie, et al., bioRxiv - Cancer Biology 2022

  Quote: ... fluorescently conjugated antibodies Ly6G (Biolegend, 5 µg, 1A8), CD45 (Biolegend ...
• An intranasal lentiviral booster broadens immune recognition of SARS-CoV-2 variants and reinforces the waning mRNA vaccine-induced immunity that it targets to lung mucosa

  Benjamin Vesin, et al., bioRxiv - Immunology 2022

  Quote: ... APC-anti-IL-5 (504306, BioLegend Europe BV), FITC-anti-IL-10 (505006 ...
• Latent Plasticity of Effector-like Exhausted CD8 T cells contributes to memory responses

  Saravanan Raju, et al., bioRxiv - Immunology 2020

  Quote: ... and 5 ug/ml of Brefeldin A (Biolegend) for 4 hours ...
• Developmental stage-specific distribution of macrophages in mouse mammary gland

  Teneale A. Stewart, et al., bioRxiv - Developmental Biology 2019

  Quote: ... rabbit anti-keratin 5 (BioLegend, 905504, batch # B230397) and rabbit anti-SMA (Abcam ...
• Distinct functions of tissue-resident and circulating memory Th2 cells in allergic airway disease

  Rod A. Rahimi, et al., bioRxiv - Immunology 2020

  Quote: ... Supernatant culture cytokine levels for IL-5 (Biolegend) and IL-13 (eBioscience ...
• Vitamin D Sufficiency Enhances Differentiation of Patient-Derived Prostate Epithelial Organoids

  Tara McCray, et al., bioRxiv - Cell Biology 2020

  Quote: ... polyclonal rabbit anti-keratin 5 (905501, BioLegend, CA), or monoclonal rabbit anti-DKK3 (ab186409 ...
• LILRB3 (ILT5) is a myeloid checkpoint on myeloid cells that elicits profound immununomodulation

  Muchaala Yeboah, et al., bioRxiv - Immunology 2020

  Quote: ... 5 μg/ml CD28 (clone CD28.2; Biolegend, UK) and 10 μg/ml LILRB3 antibodies or a relevant isotype ...
• Inhibiting fibroblast aggregation in skin wounds unlocks developmental pathway to regeneration

  Ashley W. Seifert, Adam B. Cook, Douglas Shaw, bioRxiv - Developmental Biology 2019

  Quote: ... Keratin 5 and 10 (BioLegend, San Diego, CA) 1:500 ...
• Cells of the human intestinal tract mapped across space and time

  R Elmentaite, et al., bioRxiv - Cell Biology 2021

  Quote: ... FcγRI-PE (1:200, X54-5/7.1, BioLegend), FcγRIIB-PE (1:200 ...
• Fine-tuning of β-catenin in thymic epithelial cells is required for postnatal T cell development

  Sayumi Fujimori, et al., bioRxiv - Immunology 2021

  Quote: ... chicken anti-Keratin 5 antibody (BioLegend, 1:500), and mouse anti-Keratin 8 antibody (BioLegend ...
• PD-L1 – PD-1 interactions limit effector Treg cell populations at homeostasis and during infection

  J.A. Perry, et al., bioRxiv - Immunology 2020

  Quote: ... and α-CD64 (X54-5/7.1, 139314, Biolegend) supplemented with brilliant stain buffer (566385 ...
• Splice Factor Polypyrimidine tract-binding protein 1 (Ptbp1) is Required for Immune Priming of the Endothelium in Atherogenic Disturbed Flow Conditions

  Jessica A Hensel, et al., bioRxiv - Cell Biology 2021

  Quote: ... CD4 (1:200, Biolegend 100536, clone RM4-5), CD45.2 (1:200 ...
• Neuroprotective tissue adaptation induced by IL-12 attenuates CNS inflammation

  Myrto Andreadou, et al., bioRxiv - Immunology 2022

  Quote: ... and anti-CD28 (5 μg/mL; 37.51; Biolegend) for 48-72 hours at 37°C ...
• Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption

  Anne Cooley, et al., bioRxiv - Cell Biology 2022

  Quote: ... chicken anti-Keratin 5 (1:100, Biolegend 905901), and Biotinylated goat anti-rabbit IgG (1:200 ...
• Development of SARS-CoV-2 mRNA vaccines encoding spike N-terminal and receptor binding domains

  Guillaume B.E. Stewart-Jones, et al., bioRxiv - Immunology 2022

  Quote: ... IL-5 PE (BioLegend, cat. 504303, clone TRFK5), IL-9 PerCP-Cy5.5 (BioLegend ...