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Citations for BioLegend :
651 - 700 of 7409 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and 20 ng/mL macrophage colony-stimulating factor (BioLegend, 576406). On the 4th and 6th day medium was changed to fresh ...
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bioRxiv - Genomics 2022Quote: ... ADT cocktails (diluted 20 times from original stock) from Biolegend were added onto the tissue and incubated for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... TotalSeq-A hashtag antibodies (0.5μL/sample for 20 min, BioLegend; hashtag 1 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 20 ng/mL rmIL-2 (Biolegend), 5 ng/mL m/h rTGF-β (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... and IL-4 (20 ng/ml, Biolegend, San Diego, USA). Cells were allowed to differentiate for 5-7 days (DC-SIGN+ cells in the culture > 90%).
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bioRxiv - Bioengineering 2023Quote: ... Cells were incubated with 20 ng/mL GMCSF (BioLegend 576304) at a density of 2 x 106 cells for 8 days ...
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bioRxiv - Immunology 2020Quote: ... or co-stimulated with plate-bound CD3 (Biolegend #300333) and soluble CD28 (Biolegend #302943 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were removed from their plates with Accutase (BioLegend) and counted ...
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bioRxiv - Immunology 2021Quote: ... AMs were lifted from plates using Accutase™ (BioLegend) and seeded for experiments.
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bioRxiv - Cell Biology 2021Quote: ... Azide-Free (LEAF) plate-bound anti-mouse CD3 (Biolegend) was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated overnight at 4°C with either r3CLpro-RBD chimer (2 μg/mL) ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated with r3CL-pro ...
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bioRxiv - Immunology 2022Quote: ... Plates were again washed and TMB substrate solution (BioLegend) was added for 15 minutes at room temperature prior to addition of stop solution (2M hydrochloric acid) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and developed using TMB substrate (BioLegend). Reaction was stopped using 50μL of H2SO4 ...
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bioRxiv - Immunology 2022Quote: ... plate-bound antibodies (Abs) anti-NK1.1 (clone PK136; Biolegend), anti-NKG2D (clone A10 ...
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bioRxiv - Immunology 2023Quote: ... Plates were developed with 50 μl TMB (421101, BioLegend) and stopped using 50 μl 1.5 N H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... stimulated with plate-bound anti-CD3 (Biolegend, 1μg/ml) and anti-CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2021Quote: ... fixed for 1 h at 25 °C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Immunology 2022Quote: ... fixed for 1 h at 25°C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine levels were determined using the human inflammation panel 1 LEGENDplex™ kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421 ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspensions were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... were isolated from buffy coats by density gradient centrifugation (Lymphoprep, StemCell ref: 07851) and activated on plates coated with anti-CD3 (1 mg/ml, Biolegend, ref: 317347, clone: OKT3) and anti-CD28 (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Microbiology 2022Quote: Corning ELISA plate wells were coated with 100µL of 100 μg/mL FCoV2 whole virus antigen or 100 μg/mL SCoV2 RBD or FCoV RBD antigen in sodium bicarbonate ELISA coating buffer at pH 9.5 (BioLegend, San Diego, CA) and incubated overnight at RT ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected 3 days after coculture initiation and used to quantify T cell activation levels with ELISA (BioLegend, Cat no. 430801) following the manufacturer’s protocols.
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bioRxiv - Immunology 2023Quote: ... Co-culture supernatant was collected following completion of culture and analyzed for IL-2 using the ELISA MAX™ Deluxe Set for human IL-2 (Biolegend, 431804) at a dilution of 1:5.
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bioRxiv - Microbiology 2023Quote: ... PBMCs were then plated as described above and human IL-10 was measured in the cell supernatants by ELISA (Biolegend, Cat. # 430604).
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bioRxiv - Immunology 2020Quote: ... and BMDCs were generated with rmGM-CSF (20 ng/ml, BioLegend). We used complete RPMI medium for all cell culture studies.
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bioRxiv - Cancer Biology 2022Quote: ... and interferon-γ (IFN-γ, 20 ng/mL, BioLegend, cat. 570202) was added and incubated overnight ...
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bioRxiv - Systems Biology 2019Quote: ... for 20 minutes at 4°C and Zoombie NIR (BioLegend 423105) cell viability solution at 25 ng/ml (1:2,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU-FITC antibody staining for 20 min at RT (BioLegend #364104) and 20 min incubation with PI solution (100 µg/ml ...
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bioRxiv - Genomics 2022Quote: ... and 20 ng/mL of recombinant IL-6 (BioLegend, Cat# 575702) and 1 μg/mL of soluble anti-CD28 for 6 days at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... at various doses followed by 20 ng/mL TNFα (Biolegend, 575204), 1 μg/mL LPS-PG (Invivogen ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... 20ng/mL IL-7 and 20 ng/mL IL-33(BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with 20 ng/mL IL-1β (BioLegend 579402) and 20 ng/mL TNF-α (BioLegend 570104 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured with 20 ng/mL GM-CSF (Biolegend, 576302) for 7 days with DMEM (5% pen/strep ...
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bioRxiv - Immunology 2021Quote: ... cells were restimulated with cognate antigens for three days and then supernatants were collected for IL-17A and IFNγ detection with ELISA (Biolegend, San Diego, CA). For determination of IL-17A+ and IFNγ+ CD45.1+ OT-II CD4+ T cell percentages ...
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bioRxiv - Immunology 2021Quote: Dead cells were stained using either Zombie UV™ Fixable Viability Kit (Biolegend, 1:1000), Fixable Viability Dye eFluor™ 506 (eBioscience ...
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bioRxiv - Immunology 2020Quote: Human ILC2 cells were stained with 1 μM CSFE (CSFE cell division tracker kit, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit, Biolegend, 1:500), CD45-AP/Cyanine7 (Biolegend ...