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Citations for BioLegend :
451 - 500 of 8025 citations for 2' 3' cGAMP STING based FRET Detection Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD117 (c-kit) (Biolegend, clone 104D2; 1:100); anti-human CD148 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... 1) AnnexinV/7-AAD staining (Kit from Biolegend Cat# 640922): At the end of the EMT experiment described above ...
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bioRxiv - Cancer Biology 2022Quote: ... Zombie Green™ Fixable Viability Kit (1:1000, Biolegend 423111) or DAPI (1:10000 ...
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bioRxiv - Immunology 2023Quote: ... PE-coupled anti-KIT (1:100, clone 2B8, BioLegend, #105808), FITC-coupled anti-ST2 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM EDTA then stained with anti-mouse CD31-APC (1:500, Biolegend, 102507), CD45-APC (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at 1:100 dilution in 2% BSA-PBS prior to using streptavidin BV421 (405225, BioLegend) and anti-human VRC01 mAb conjugated to AF488.
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL, VCAM-1, 429 MVCAM.A, BioLegend) were used ...
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bioRxiv - Immunology 2023Quote: ... hydrogel beads were stained with anti-CD138 PE (1:80 dilution, clone 281-2, BioLegend), anti-FLAG PE-Cy7 (1:200 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Western blot analysis of the samples were done using anti- HA (1:2 000; BioLegend) and anti-Flag M2 (1:5 000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were stained for CD69 (clone FN50, BioLegend, dilution 1:500 in PBS + 2% FBS) expression after 4 - 24 hr at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Zombie NIR Viability dye was diluted based upon Biolegend’s & Cytek’s suggested protocol before staining samples at room temperature for 15 minutes (Biolegend #423105) in 1.5mL Eppendorf tubes ...
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bioRxiv - Immunology 2020Quote: ... A 13-plex bead-based immunoassay was performed using the manufacturer’s instructions (Legendplex Th Panel, Biolegend, San Diego, CA). All samples were acquired using Novocyte flow cytometer (ACEA Biosciences ...
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bioRxiv - Microbiology 2022Quote: Serum cytokine levels were determined using a beads-based immunoassay with a custom-designed mouse cytokine panel (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: IL-1β release was measured in murine serum using the LEGENDplex™ mouse inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as described above except shaking was performed at 800 rpm.
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bioRxiv - Microbiology 2021Quote: ... cytokines were measured from effluent at the vascular side using multiplex bead-based immunoassays (LEGENDplex, BioLegend, SanDiego, CA, USA). All samples were measured in duplicates for every donor and experiment using a BD Canto II flow cytometer (BD ...
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bioRxiv - Immunology 2022Quote: ... B cell purity was > 95% based on flow cytometric analysis following staining with anti-CD19 (Biolegend, San Diego, CA). FO and MZ B cells were >90% pure based on the criteria that they were sorted on.
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bioRxiv - Immunology 2023Quote: ... Primary mouse lung fibroblasts were freshly isolated after magnetic bead-based negative selection of epithelial cells (118204, Biotin anti-mouse CD326 Epcam, Clone: G8.8, BioLegend), endothelial cells (102404 ...
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bioRxiv - Neuroscience 2023Quote: ... using a cell-based assay according to manufacturer’s instructions (EUROIMMUN, Lübeck, Germany) with a secondary Anti-mouse-IgG Antibody (Biolegend, Poly4053). Dilution steps included pure ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Cancer Biology 2019Quote: ... Purified cells were plated at 1×105 cells per well on a 96 well plate coated with 10μg/ml αCD3 (Biolegend), and cultured with 2μg/ml soluble αCD28 (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend, 421101). The reaction was stopped with 1 M phosphoric acid after 20 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 1ug/ml) and plate bound anti-CD3 (500 ug/ml) in the presence of Brefeldin A (1:1000, Biolegend) for 4 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with FACS-wash (FW-1% FBS in PBS) in 96-well V-shape plates and stained with DAPI (0.1 µg/ml) (Biolegend) for 5 minutes ...
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bioRxiv - Pathology 2022Quote: An uncoated ELISA plate (423501, Biolegend) was coated with 1.2 μg/mL HERV-K113 Capsid protein (sp|Q902F9|90-632 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Immunology 2020Quote: (i) Cholesterol and glycosphingolipid detection: CD4-BV711 (clone RPA-T4, BioLegend Cat# 300558, RRID:AB_2564393), CD25-BV510 (clone MA251 ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used for detection: anti-FMRP (MAB2160, Millipore; 6B8, Biolegend), and anti-Calnexin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... A biotinylated primary antibody was used for direct detection without signal amplification (Biolegend 339809). Positive staining was confirmed by reference to the testing information provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of Aβ species was achieved using the mouse monoclonal HRP-conjugated 6E10 (Biolegend) antibody at a dilution of 1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... with a secondary (anti-rat) detection antibody labeled with Alexa Fluor-647 (Biolegend, 407511) at a 1:500 dilution ...
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bioRxiv - Immunology 2023Quote: ... Capture and biotin conjugated detection anti-mouse IL-4 antibodies were purchased from BioLegend. Anti-mouse IL-13 antibody pair were purchased from Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...