Labshake search
Citations for BioLegend :
451 - 500 of 591 citations for 10 UNDECENYLDIMETHYLCHLOROSILANE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
-
bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
-
bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
-
bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
-
bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
-
bioRxiv - Cell Biology 2023Quote: ... washed with PBS 1% FBS and incubated 10 mins at RT with Human TruStain FcX and True-Stain Monocyte blocker (Biolegend). Antibody mix containing CD14-APCFire750 ...
-
bioRxiv - Microbiology 2023Quote: ... for 30 minutes at 37°C and then incubated with 10 µg/ml of αASC TMS-1 (clone HASC-71, Biolegend) in blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... the appropriate number of wells of a flat-bottomed tissue culture plate were coated with 10 μg/mL αCD3 (Biolegend) in PBS for 3h at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
-
bioRxiv - Microbiology 2023Quote: ... samples were stained with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with HBSS (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 500 µl RPMI containing 10% FBS and labelled with commercially available DNA-labelled antibodies (TotalSeq-A, Biolegend) at 4 °C for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... cells from the dermis and epidermis were combined and cultured overnight in the presence of 10 U/ml IL-2 (Biolegend). The following antibodies were used in flow analysis of skin associated γδ T cell populations ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with the following antibodies:(CEACAM6-FITC at 10 μg/mL, Sino Biological 10823-R408R; H2Kb-Pac Blue at 0.5 mg/mL, BioLegend 116514 ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by staining with Brilliant Violet (BV) 785 conjugated anti-human CD69 (10 μgꞏml−1) (Biolegend, Cat # 310932, clone FN50) for 30 min on ice ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 10 ng/mouse of rmIL-12 (R&D) or 100 ng/mouse of rmIL-33 (Biolegend) alone or in combination in 50 µL directly in the tumor area twice a week from day 2 to day 26 ...
-
bioRxiv - Immunology 2023Quote: ... After blocking of free capture sites on the biosensors with an isotype control (10 µg/mL, human IgG1κ, clone QA16A12, BioLegend) for 600 s ...
-
bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 100 μl chilled DPBS with 0.2% BSA and 10 μl of human TrueStain FcX (BioLegend, 422302) and incubated on ice for 10 min to reduce non-specific labeling ...
-
bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
-
bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
-
bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
-
bioRxiv - Immunology 2023Quote: ... followed by staining with a premixed cocktail of the indicated tetramers at a final concentration of 10 nM for 1 hour at RT in the presence of anti-mouse CD16/32 antibody (clone 93, BioLegend). SLO preparations were magnetically enriched for tetramer-specific cells as previously described64 while lung samples were magnetically enriched for CD90.2 expression (Miltenyi) ...
-
bioRxiv - Immunology 2022Quote: ... For fluorescence staining the following primary/secondary antibodies and dyes diluted in PBS/10%NGS were used: α-EpCam/CD326 (clone G8.8, Biolegend), α-cleaved Caspase 3 (#9661 ...
-
bioRxiv - Cell Biology 2023Quote: ... Neurons were then maintained for 10 min at 37°C in the presence of AlexaFluor-647-conjugated AnxA5 (1/50, Biolegend) in resting solution (Locke’s solution:140 mM NaCl ...
-
bioRxiv - Neuroscience 2023Quote: ... the following antibodies were added in a 10 µl master mix: CD11b-FITC (M1/70, 1:400, 1:400, Biolegend), CD45-APC/Cy7 (30F11 ...
-
bioRxiv - Immunology 2023Quote: ... cells were fixed with 4% PFA for 10 min at RT followed by permeabilization with the True-Phos Perm buffer (Biolegend) for 20 min on ice ...
-
bioRxiv - Biochemistry 2023Quote: ... Cell pellets were collected and resuspend by 500 μL (for 80 mL cord blood) Dilution Buffer and then incubated by 10 μL CD34-APC antibody (343510, BioLegend) on ice for 30 min ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
-
bioRxiv - Immunology 2023Quote: ... A scaffold-only tetramer was also made with biotinylated S2hlx-Ex19-PShlx or FP-10-wt mixed with Streptavidin-BV421 (Biolegend). These probes did not contain the grafted epitope.
-
bioRxiv - Immunology 2023Quote: Peyer’s patch leukocytes from 10 WT B6 mice de novo colonized for 21 days with SFB were separately stained with oligo-hashtagged antibodies (TotalSeqC 1 – 10, Biolegend), prior to pooling ...
-
bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
-
bioRxiv - Immunology 2023Quote: ... Single cell suspensions were stained as above with efluor780 viability dye prior to incubation at 37C for 10 min with CCR7-APC-Fire810 (clone G043H7, Biolegend) and FcγR block ...
-
bioRxiv - Immunology 2023Quote: ... The sorted CD45+ cells were stained with the TotalSeq™ antibody mixture according to manufacturer’s protocol (BioLegend, Suppl. Table 10). CD4+ memory T cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
-
bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
-
bioRxiv - Microbiology 2023Quote: ... BMDMs were stimulated for 2 hours prior to infection with vehicle or 10 ng/mL of each cytokine: GM- CSF (Biolegend), M-CSF (Miltenyi) ...
-
bioRxiv - Cell Biology 2023Quote: ... and cultured in DMEM/F-12 medium containing 10% FBS and 20 ng/mL mouse macrophage colony-stimulating factor (BioLegend) for 7 days ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
-
bioRxiv - Cancer Biology 2024Quote: ... mesothelioma cell lines were further pre-treated with anti-MHC class I antibody (10 μg/ml) (Clone W6/32, Biolegend) for 1 hour ...
-
bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
-
bioRxiv - Immunology 2024Quote: ... or gE overlapping peptides (10 μg/mL, 153 peptides, 15mers with 11 aa overlap) in the presence of brefeldin A (BFA, Biolegend) for 8 hours or 16 hours at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... cells were split into 2 new 96-well plates and incubated with freshly prepared supplemented RPMI media with IL-2 (10 ng/ml, BioLegend, #575402). Cells were cultured for a total of 5 days before injection ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
-
bioRxiv - Immunology 2019Quote: ... cells were permeabilized and stained with IL-10 (clone JES5-16E3, eBioscience) or isotype control (Rat IgG2b Κ Isotype Control, Biolegend). Fluorescence intensities were measured on a FACSCalibur and at least 25,000 events for lung tissue were analyzed using FlowJo.
-
bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
-
bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801 ...
-
bioRxiv - Cell Biology 2019Quote: ... insulin-like growth factor 2 (IGF2; 20 ng/ml) and fibroblast growth factor (FGF; 10 ng/ml) (BioLegend or StemCell Technologies) along with 5ug/ml polybrene (Sigma ...