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Citations for BioLegend :
551 - 600 of 7858 citations for 1 2 Bis Pentabromophenyl Ethane Unlabeled 25 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and resuspended in 25 µl of TotalSeq-C antibody cocktail (Biolegend; Table S14). Cells were incubated on ice for 30 minutes and washed 4 times in 1.5 ml FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 25 min with biotinylated anti-human MMR (321118, BioLegend), washed ...
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bioRxiv - Immunology 2020Quote: ... IL-2 immune complex (IL-2ic; mouse recombinant IL-2 + anti-IL-2 (JES6-1A12, BioLegend); molar ratio 2:1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then permeabilised with Saponin 2% in PBS for 20 min at room temperature and then stained in Saponin 2% in PBS with the appropriate antibodies: Th-1 (anti-CD3-BV510 (1:100, Biolegend, Cat#300448), anti-CD4-PE (1:100 ...
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bioRxiv - Immunology 2022Quote: ... with 100 μg ml−1 of streptavidin-PE (eBiosciences) or streptavidin-BV421 (BioLegend), respectively ...
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bioRxiv - Bioengineering 2019Quote: ... The 200μL of 1:100 suspension APC dye-labeled streptavidin (Biolegend, 5μg/mL) were added onto glass slide to detect the 635-nm detection antibody group ...
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bioRxiv - Microbiology 2022Quote: ... containing 1 μg/ml of the antibodies anti-mouse EpCam (PE-Cy7, Biolegend), anti-mouse Podoplanin (PE ...
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bioRxiv - Immunology 2020Quote: ... transforming growth factor (TGF)-β1 (1 ng/mL, Biolegend, San Diego, CA, USA), IL-23 (10 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 1 μg/mL biotinylated anti-CD28 antibody (Clone 37.15, BioLegend) PBS+2%FCS for 30 minutes on ice to allow binding ...
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bioRxiv - Immunology 2023Quote: ... stimulated with 1 μg/ml of plate-bound anti-CD3 (145-2C11, Biolegend), 2 μg/ml anti-CD28 (37.5 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL of 0.5 mg/mL totalseq-C streptavidin (Biolegend, 405271, 405273, 405275) per 6 μL nanovial volume was additionally added during the streptavidin conjugation step.
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bioRxiv - Cancer Biology 2023Quote: PE-Cy7 anti-mouse/rat CD29 (Biolegend, #102222, 0.2 mg/ml, 1:100)
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bioRxiv - Cancer Biology 2023Quote: ... T cells were activated with 1 μg/ml of anti-CD3e [OKT3] (BioLegend) and 1 μg/ml of anti-CD28 (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... and only in the presence of 1 ng/mL IL-7 thereafter (BioLegend). Cultures were analyzed and passaged every 3 days.
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bioRxiv - Cell Biology 2024Quote: ... Red blood cells were removed by adding 1 mL RBC lysis buffer (Biolegend) to each tube and incubate at room temperature for 1 minute.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Genomics 2022Quote: ... at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend) and pooled equally as described previously18 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend), CD127 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/ml erythropoietin) for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID ...
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bioRxiv - Immunology 2021Quote: ... Heparinized blood diluted 1:1 in PBS overlaid onto Lymphopure density gradient medium 1.077 g/mL (BioLegend, San Diego, USA) in Leucosep tubes (Greiner Bio-One ...
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bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
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bioRxiv - Immunology 2023Quote: ... Stained T-IEL and unstained lymph node T cells were mixed at a 1:1 ratio before stimulation with 30 μg/ml anti-CD3 (Clone 145-2C11, Biolegend) crosslinked with 5 μg/ml anti-Hamster (Jackson Immunoresearch) ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA and actin were stained with 1 μg/mL DAPI and phalloidin (1:1000, Flash Phalloidin™ Red 594; BioLegend). Testis were washed three times with PBS and mounted in (ProLong™ Gold Antifade Mountant ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostaining was performed with biotinylated monoclonal mouse antitubulin (clone 236-10501, Thermo, 1 μg/ml) then Brilliant Violet 421-conjugated streptavidin (Biolegend, 0.1 μg/ml), Alexa Fluor 488-conjugated mouse anti-perforin (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... T cells were added to anti-CD3 coated wells at 106 cells/mL in the presence of 1 μg/mL anti-CD28 (Biolegend, LEAF purified, 37.51), 100 U/ml IL-2 (gift from K ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Immunology 2019Quote: ... according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 μg/mL anti-human RP105 antibody clone MHR73-11 (BioLegend Cat.# 312907).
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bioRxiv - Microbiology 2022Quote: ... then stained for 30 minutes at 2-8°C with the following cytokine antibodies: Granzyme B (GzB) BV421 (4 μg/mL, BioLegend Cat. 396414), IFN-gamma (IFN-γ ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured overnight with plate-bound anti-CD3 (OKT3, 1 μg/ml, Biolegend) and soluble anti-CD28 (CD28.2 ...
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bioRxiv - Microbiology 2022Quote: ... wells were stained with Zombie Viability Dye Aqua (1 μL/mL, BioLegend Cat. 423101) for 15 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... then incubated with cell viability marker propidium iodide (PI, 1 μg/mL, Biolegend 421301). Flow cytometry was performed on a FACS Aria II (BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Immunology 2023Quote: ... biotin anti-CD3 (7 μg/mL) Abs for 1 h at room temperature (BioLegend, clones RA3-6B2 ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Developmental Biology 2023Quote: ... cell pellets were resuspended with 1 ml of cold RBC lysis buffer (Biolegend, 420301) to lyse the remaining erythrocytes for 5 min on ice ...