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• RAD51AP1 and RAD54 underpin two distinct RAD51-dependent routes of DNA damage repair via homologous recombination

  Platon Selemenakis, et al., bioRxiv - Cell Biology 2021

  Quote: ... α-HA.11 (MMS-101R; BioLegend; 1:1,000) and α-RAD54B ((Wesoly et al ...
• LAP1 supports nuclear plasticity during constrained migration

  Yaiza Jung-Garcia, et al., bioRxiv - Cell Biology 2021

  Quote: ... HA.11 (1:500, #901503) from BioLegend; Gamma-H2AX (1:600 ...
• Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains

  Rachael E. Barton, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-HA 11 (Biolegend,; 1:1000), mouse anti-FLAG M2 (Sigma- ...
• Propagation of neuronal micronuclei regulates microglial states

  Sarasa Yano, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-CD68 (FA-11, Biolegend, 1:500), anti-Lamin B1 (ab16048 ...
• Sequential early-life viral infections modulate the microbiota and adaptive immune responses to systemic and mucosal vaccination

  Yuhao Li, et al., bioRxiv - Microbiology 2023

  Quote: ... IgD (Biolegend, 11-26c.2a,1:200,), Ter119(Biolegend ...
• Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity

  Shilpa Singh, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 11 epitope tag (BioLegend #901501, 1:1000).
• Sensitive detection and propagation of brain-derived tau assemblies in HEK293 based wild-type tau seeding assays

  Melissa Huang, William A McEwan, bioRxiv - Molecular Biology 2024

  Quote: ... anti-HA.11 (Biolegend, 901516l; 1:2000); anti-GAPDH (ThermoScientific ...
• AAV-delivery of diacylglycerol kinase kappa achieves long-term rescue of Fmr1-KO mouse model deficits of fragile X syndrome

  Karima Habbas, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-HA.11 16B12 (1:500, Biolegend), mouse anti-NeuN (1:500 ...
• The activity of engrailed imaginal disc enhancers is modulated epigenetically by chromatin and autoregulation

  Yuzhong Cheng, Fountane Chan, Judith A. Kassis, bioRxiv - Developmental Biology 2023

  Quote: ... anti-HA.11 (1:1000, clone 16B12, Biolegend), rabbit anti-Gal4 activation domain (1:1000 ...
• Oligodendrocyte-derived IL-33 functions as a microglial survival factor during neuroinvasive flavivirus infection

  Geoffrey T. Norris, et al., bioRxiv - Immunology 2023

  Quote: ... rat anti-CD68 (1:300; FA-11; Biolegend), Goat anti-IL-33 (AF3626 ...
• Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase

  Ruipeng Lei, et al., bioRxiv - Microbiology 2022

  Quote: ... Cells were incubated with 1 μg mL-1 PE-anti HA.11 (BioLegend) at 4 °C with rocking for 1 h ...
• A ubiquitin-based mechanism for the oligogenic inheritance of heterotaxy and heart defects

  Jennifer H. Kong, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-HA.11 (clone 16B12, BioLegend, 1:2000); mouse anti-HA (clone 2-2.2.14 ...
• Mechanism of in vivo activation of the MutLγ-Exo1 complex for meiotic crossover formation

  Aurore Sanchez, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HA.11 mouse monoclonal antibody (16B12, Biolegend, 1/750) or TAP rabbit monoclonal antibody (Invitrogen ...
• AAV-delivery of diacylglycerol kinase kappa achieves long-term rescue of Fmr1-KO mouse model deficits of fragile X syndrome

  Karima Habbas, et al., bioRxiv - Neuroscience 2021

  Quote: ... purified mouse anti-HA.11 16B12 (1:5000, Biolegend), rabbit anti-hDGKk (1:1000 ...
• Lung-resident memory B cells maintain allergic IgE responses in the respiratory tract

  Alexander J. Nelson, et al., bioRxiv - Immunology 2024

  Quote: ... IgD (1:250, PE, clone 11-26c.2a, BioLegend) in PBS plus 10% RS overnight at 4⁰ C in a humidified chamber ...
• CD38 regulates ovarian function and fecundity via NAD⁺ metabolism

  Rosalba Perrone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD68-AF700 (BioLegend, clone FA-11, 137025, 1:200); IgM-APC-Cy7 (BioLegend ...
• PKC modulator bryostatin-1 therapeutically targets CNS innate immunity to attenuate neuroinflammation and promote remyelination

  Payam Gharibani, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-CD45 BV605 (1:400; clone 30-11; Biolegend), anti-Ly6G (1:400 ...
• BARD1 links histone H2A Lysine-15 ubiquitination to initiation of BRCA1-dependent homologous recombination

  Jordan R. Becker, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and mouse anti-HA (1:2000, HA.11 901501 Biolegend). Following primary ...
• Extrafollicular responses are sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus

  Lasse F. Voss, et al., bioRxiv - Immunology 2022

  Quote: ... IgD-AF488 clone 11-26c.2a (405718, BioLegend, 1:500), Ki67-eflour660 clone SolA15 (50-5698-82 ...
• Extrafollicular responses are sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus

  Lasse F. Voss, et al., bioRxiv - Immunology 2022

  Quote: ... IgD-AF488 clone 11-26c.2a (405718, BioLegend, 1:500), IgMb-BV510 clone AF6-78 (742344 ...
• CD38 regulates ovarian function and fecundity via NAD⁺ metabolism

  Rosalba Perrone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... IgD-Alexa_Fluor_647 (BioLegend, clone 11-26c.2a, 405707, 1:800), CD68-AF700 (BioLegend ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgD-AF700 (1:200, 11-26c.2a, Biolegend), anti-mouse IgM-PE/Cy7 (1:200 ...
• Haptenic adducts of β-lactam antibiotics elicit antibody responses with narrow clonality and specificity

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgD-AF700 (1:200, 11-26c.2a, Biolegend), anti-mouse IgM-PE/Cy7 (1:200 ...
• Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase

  Ruipeng Lei, et al., bioRxiv - Microbiology 2022

  Quote: ... washed with 1 mL FACS buffer and incubated with 1 μg mL-1 PE-anti HA.11 (BioLegend) at 4 °C with rocking for 1 h ...
• Macrophage WDFY3, a protector against autoimmunity

  Xun Wu, et al., bioRxiv - Immunology 2024

  Quote: ... CD8-FITC (eBioscience, 11-0083-85, 1:100) or CD4-FITC (Biolegend, 100406, 1:100). CD4+ T cells were recognized as CD45+Ly6-C/G-CD115-TCRβ+CD4+ or CD45+Ly6-C/G-CD115-TCRβ+CD8- ...
• Donor Macrophages Modulate Rejection after Heart Transplantation

  BJ Kopecky, et al., bioRxiv - Immunology 2021

  Quote: ... anti-mouse CD68 1:200 clone FA-11 (Biolegend Cat# 137002), anti-Ki-67 1:200 (Invitrogen Cat# 14-5698-82 ...
• PTEN Inhibition Ameliorates Muscle Degeneration and Improves Muscle Function in a Mouse Model of Duchenne Muscular Dystrophy

  Feng Yue, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Alexa Fluor 647 anti-mouse CD68 (FA-11, BioLegend, 1:1,000), or anti-mouse MG53 (gift from Jianjie Ma ...
• Muscle Van Gogh-like 2 shapes the neuromuscular synapse by regulating MuSK signaling activity

  Myriam Boëx, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-HA.11 (1:100 for immunoprecipitation, Biolegend, catalog no. 902301), anti-HA (1:2500 for Western blotting ...
• Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies

  Ariel K. Frame, et al., bioRxiv - Neuroscience 2022

  Quote: ... and mouse anti-HA.11 epitope tag (901513, BioLegend; 1:1000). Subsequently ...
• Opposing diet, microbiome and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host

  Gabriel Vasconcelos Pereira, et al., bioRxiv - Microbiology 2023

  Quote: ... PerCP-Cy5.5-conjugated anti-IgD (Biolegend, 11-26c.2a; 1/200), PE-conjugated anti-IgE (Biolegend ...
• Inhibition of CD226 Co-Stimulation Suppresses Diabetes Development in the NOD Mouse by Augmenting Tregs and Diminishing Effector T Cell Function

  Matthew E. Brown, et al., bioRxiv - Immunology 2024

  Quote: ... and plate-bound 11-CD28 (BioLegend; RRID: AB_11147170; 1 µg/mL) or plate-bound 11-CD3 (BioLegend ...
• Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing

  Irene Carrozzo, et al., bioRxiv - Bioengineering 2024

  Quote: Cells were stained with HA.11 antibody (#901515, Biolegend 1:1000) at 4°C for 45 minutes ...
• Enhancing cancer targeting of γ9δ2TCR through modified NKG2D co-stimulation

  Patricia Hernández-López, et al., bioRxiv - Immunology 2021

  Quote: ... αβTCR-PE-Cy5 (1:80; clone IP26; 11-9986-42) from Biolegend; γδTCR-PE-Cy7 (1:20 ...
• The Alzheimer’s Disease Risk Genes MS4A4A And MS4A6A Cooperate to Negatively Regulate Trem2 and Microglia states

  Dalya Rosner, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse IgG2b-PE isotype control (Clone MPC-11; 1:200; Biolegend #400312), TREM2-DL650 (Clone T22 ...
• LDL-Binding IL-10 Reduces Vascular Inflammation in Atherosclerotic Mice

  Lisa R. Volpatti, et al., bioRxiv - Bioengineering 2024

  Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
• Inhibition of selective autophagy by members of the herpesvirus ubiquitin-deconjugase family

  Päivi Ylä-Anttila, Maria G. Masucci, bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-HA.11 clone 16B12 (WB 1:1000, 901501) was from Biolegend; mouse anti-HA clone 12CA5 (WB 1:2000 ...
• Sequence signatures of two IGHV3-53/3-66 public clonotypes to SARS-CoV-2 receptor binding domain

  Timothy J.C. Tan, et al., bioRxiv - Microbiology 2021

  Quote: ... 1 μg of PE anti-HA.11 (epitope 16B12, BioLegend, Cat. No. 901517) buffer-exchanged into 1x PBS was added to the cells ...
• Inflammation perturbs hematopoiesis by remodeling specific compartments of the bone marrow niche

  James W. Swann, et al., bioRxiv - Cell Biology 2024

  Quote: ... Flt3-PE (eBioscience,12-1351-82; 1:100), CD34-FITC (eBioscience, 11-0341-85; 1:25) and CD16/32-PE/Cy7 (BioLegend, 101318; 1:800), along with lineage antibodies in PE/Cy5 (Gr1 [eBioscience ...
• Hormonal gatekeeping via the blood brain barrier governs behavior

  Linyang Ju, et al., bioRxiv - Neuroscience 2022

  Quote: ... Antibodies used were as follows: 1:1000 mouse anti-HA.11 epitope (16B12, BioLegend); 1:2000 mouse anti-β-tubulin (E7 ...
• Decreasing Mitochondrial RNA Polymerase Activity Reverses Biased Inheritance of Hypersuppressive mtDNA

  Daniel Corbi, Angelika Amon, bioRxiv - Cell Biology 2021

  Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
• Divergent cytokine and transcriptional signatures control functional T follicular helper cell heterogeneity

  Lennard Dalit, et al., bioRxiv - Immunology 2024

  Quote: ... clone GK1.5-7; WEHI Antibody Facility); anti-IgD (1:200; clone 11-26c; eBioscience) and anti-GL7 (1:100; clone GL7; Biolegend).
• IL-1β Signalling Modulates T Follicular Helper and Regulatory Cells in Human Lymphoid Tissues

  Romain Vaineau, et al., bioRxiv - Immunology 2024

  Quote: ... CTLA-4 (BV605, 1/40, BioLegend), PD-1 (PE-Cy5.5 ...
• A recurring YYDRxG pattern in broadly neutralizing antibodies to a conserved site on SARS-CoV-2, variants of concern, and related viruses

  Hejun Liu, et al., bioRxiv - Immunology 2021

  Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
• Dual role of brain endothelial Gpr126 in blood-brain barrier development and ischemic stroke

  Nikolaos Kakogiannos, et al., bioRxiv - Cell Biology 2022

  Quote: ... BV421 Rat anti mouse CD68 (Monoclonal; clone FA-11; Biolegend; 1:300; Cat#137017, FACS). AlexaFluor700 Rat anti mouse CD206 (Monoclonal ...
• G protein-coupled receptor 17 inhibits glucagon-like peptide-1 secretion via a Gi/o-dependent mechanism in enteroendocrine cells

  Jason M. Conley, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Cells were incubated in primary antibody (1:1000 anti-HA.11 mouse, Biolegend clone 16B12) overnight at 4°C and incubated in secondary antibody (1:500 DyLight 488 goat anti-mouse ...
• Rhes, a Striatal Enriched Protein, Regulates Post-Translational Small-Ubiquitin-like-Modifier (SUMO) Modification of Nuclear Proteins and Alters Gene Expression

  Oscar Rivera, et al., bioRxiv - Biochemistry 2020

  Quote: ... HA.11 Epitope Tag Antibody (1:1000, 901513) was from BioLegend (previously Covance catalog# MMS-101R). V5-Tag (1:1000 ...
• Cryo-EM Structure of Native Human Uromodulin, a Zona Pellucida Module Polymer

  Alena Stsiapanava, et al., bioRxiv - Biophysics 2020

  Quote: ... using mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:1,000 dilution), mouse anti-β-actin monoclonal (clone AC-74 ...
• A booster dose enhances immunogenicity of the COVID-19 vaccine candidate ChAdOx1 nCoV-19 in aged mice

  Alyssa Silva-Cayetano, et al., bioRxiv - Immunology 2020

  Quote: ... which included AF647-conjugated rat anti-mouse IgD (clone 11-26 c.2a, Biolegend; 1:200), FITC-conjugated rat anti-mouse Ki67 (clone SolA15 ...
• Coordinated changes in glycosylation regulate the germinal centre through CD22

  Jhon R. Enterina, et al., bioRxiv - Immunology 2021

  Quote: ... Mouse IL-4 (1 ng/mL; BioLegend) was added to primary co-culture for 4 days ...
• Transposable elements and their KZFP controllers are drivers of transcriptional innovation in the developing human brain

  Christopher J. Playfoot, et al., bioRxiv - Genomics 2020

  Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...