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• scRNA-seq reveals transcriptional dynamics of Encephalitozoon intestinalis parasites in human macrophages

  Pattana Jaroenlak, et al., bioRxiv - Microbiology 2024

  Quote: ... 1 μl of TotalSeq™ B anti-human hashtag antibodies 5-10 (BioLegend, USA) was added into the cell suspensions collected from each infection time point and incubated for 20 min at 4°C ...
• Dissecting the role of CAR signaling architectures on T cell activation and persistence using pooled screening and single-cell sequencing

  Rocío Castellanos-Rueda, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... 50,000 CAR-T cells were co-cultured with 100,000 target cells for 5h in the presence of CD107a antibody (Supp. Table 3) and 1x Brefeldin A (Biolegend). Following co-culture ...
• IL-6 and IL-27 negatively regulate CRTAM-expressing CD4⁺ T-cells associated with lymphoid-driven synovitis

  Alicia Derrac Soria, et al., bioRxiv - Immunology 2024

  Quote: ... and CD355/CRTAM (Clone 11-5; BioLegend).
• Interleukin-33 from oligodendrocytes sustains effector differentiation of tissue-resident CD8+ T cells and is a druggable target in CNS autoimmune disease

  Nicolas Fonta, et al., bioRxiv - Immunology 2024

  Quote: ... isolated leukocytes were cultured for 5h in the presence of Monensin (BioLegend) and Brefeldin (BioLegend) ...
• Acute irradiation alters the heterogeneity among medullary thymic epithelial cells

  Kenta Horie, et al., bioRxiv - Immunology 2023

  Quote: Antibodies and reagents employed included APCCy7-anti-mouse CD45 (BioLegend, clone 30 F-11, Cat#103116), APCCy7-anti-mouse TER119 (BioLegend ...
• A Prime/Pull RR2/CXCL11 Therapeutic Vaccine that Bolsters the Number and Function of Dorsal Root Ganglia Tissue-Resident HSV-Specific CD8⁺ T_RM Cells Protects Latently Infected Guinea Pigs from Recurrent Genital Herpes

  Nisha Dhanushkodi, et al., bioRxiv - Immunology 2022

  Quote: ... anti-mouse CRTAM (clone 11-5, Biolegend, San Diego, CA), hamster anti-mouse PD- 1 clone J43 ...
• Therapeutic Prime/Pull Vaccination of HSV-2 Infected Guinea Pigs with the Ribonucleotide Reductase 2 (RR2) Protein and CXCL11 Chemokine Boosts Antiviral Local Tissue-Resident and Effector Memory CD4⁺ and CD8⁺ T Cells and Protects Against Recurrent Genital Herpes

  Afshana Quadiri, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse CRTAM (clone 11-5, Biolegend, San Diego, CA), hamster anti-mouse PD-1 clone J43 ...
• SunCatcher: Clonal Barcoding with qPCR-Based Detection Enables Functional Analysis of Live Cells and Generation of Custom Combinations of Cells for Research and Discovery

  Qiuchen Guo, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and PD-L1 (clone: 10.F.9G2, PE-Cy7, 1:100 dilution BioLegend) on ice for 30 minutes ...
• RAD51AP1 and RAD54 underpin two distinct RAD51-dependent routes of DNA damage repair via homologous recombination

  Platon Selemenakis, et al., bioRxiv - Cell Biology 2021

  Quote: ... α-HA.11 (MMS-101R; BioLegend; 1:1,000) and α-RAD54B ((Wesoly et al ...
• LAP1 supports nuclear plasticity during constrained migration

  Yaiza Jung-Garcia, et al., bioRxiv - Cell Biology 2021

  Quote: ... HA.11 (1:500, #901503) from BioLegend; Gamma-H2AX (1:600 ...
• Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains

  Rachael E. Barton, et al., bioRxiv - Cell Biology 2021

  Quote: ... mouse anti-HA 11 (Biolegend,; 1:1000), mouse anti-FLAG M2 (Sigma- ...
• Propagation of neuronal micronuclei regulates microglial states

  Sarasa Yano, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-CD68 (FA-11, Biolegend, 1:500), anti-Lamin B1 (ab16048 ...
• Sequential early-life viral infections modulate the microbiota and adaptive immune responses to systemic and mucosal vaccination

  Yuhao Li, et al., bioRxiv - Microbiology 2023

  Quote: ... IgD (Biolegend, 11-26c.2a,1:200,), Ter119(Biolegend ...
• Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity

  Shilpa Singh, et al., bioRxiv - Cancer Biology 2024

  Quote: ... 11 epitope tag (BioLegend #901501, 1:1000).
• Sensitive detection and propagation of brain-derived tau assemblies in HEK293 based wild-type tau seeding assays

  Melissa Huang, William A McEwan, bioRxiv - Molecular Biology 2024

  Quote: ... anti-HA.11 (Biolegend, 901516l; 1:2000); anti-GAPDH (ThermoScientific ...
• Interferon-γ driven differentiation of monocytes into PD-L1⁺ and MHC II⁺ macrophages and the frequency of Tim-3⁺ tumor-reactive CD8⁺ T cells within the tumor microenvironment predict a positive response to anti-PD-1-based therapy in tumor-bearing mice

  Jelena Gabrilo, et al., bioRxiv - Immunology 2024

  Quote: ... Five different TotalSeq-B antibodies were used in each experiment (Hashtag antibody 1-5, BioLegend). Incubation lasted for 30 min ...
• AAV-delivery of diacylglycerol kinase kappa achieves long-term rescue of Fmr1-KO mouse model deficits of fragile X syndrome

  Karima Habbas, et al., bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-HA.11 16B12 (1:500, Biolegend), mouse anti-NeuN (1:500 ...
• The activity of engrailed imaginal disc enhancers is modulated epigenetically by chromatin and autoregulation

  Yuzhong Cheng, Fountane Chan, Judith A. Kassis, bioRxiv - Developmental Biology 2023

  Quote: ... anti-HA.11 (1:1000, clone 16B12, Biolegend), rabbit anti-Gal4 activation domain (1:1000 ...
• Oligodendrocyte-derived IL-33 functions as a microglial survival factor during neuroinvasive flavivirus infection

  Geoffrey T. Norris, et al., bioRxiv - Immunology 2023

  Quote: ... rat anti-CD68 (1:300; FA-11; Biolegend), Goat anti-IL-33 (AF3626 ...
• BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection

  Yunlong Cao, et al., bioRxiv - Immunology 2022

  Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
• Chitinase 3-like-1 Stimulates PD-L1 and Other Immune Checkpoint Inhibitors

  Bing Ma, et al., bioRxiv - Cancer Biology 2021

  Quote: ... granzyme-B (1:1000) (Biolegend) and PTEN (1:1000 ...
• Ubiquitin-specific protease 8 controls B cell proteostasis and cell survival in multiple myeloma

  Almut Dufner, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and anti-mouse CD40 antibody (5 µg ml-1; HM40-3, Biolegend), or anti-IgM F(ab’)₂ fragment (5 µg ml-1 ...
• A ubiquitin-based mechanism for the oligogenic inheritance of heterotaxy and heart defects

  Jennifer H. Kong, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-HA.11 (clone 16B12, BioLegend, 1:2000); mouse anti-HA (clone 2-2.2.14 ...
• Mechanism of in vivo activation of the MutLγ-Exo1 complex for meiotic crossover formation

  Aurore Sanchez, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HA.11 mouse monoclonal antibody (16B12, Biolegend, 1/750) or TAP rabbit monoclonal antibody (Invitrogen ...
• AAV-delivery of diacylglycerol kinase kappa achieves long-term rescue of Fmr1-KO mouse model deficits of fragile X syndrome

  Karima Habbas, et al., bioRxiv - Neuroscience 2021

  Quote: ... purified mouse anti-HA.11 16B12 (1:5000, Biolegend), rabbit anti-hDGKk (1:1000 ...
• Lung-resident memory B cells maintain allergic IgE responses in the respiratory tract

  Alexander J. Nelson, et al., bioRxiv - Immunology 2024

  Quote: ... IgD (1:250, PE, clone 11-26c.2a, BioLegend) in PBS plus 10% RS overnight at 4⁰ C in a humidified chamber ...
• CD38 regulates ovarian function and fecundity via NAD⁺ metabolism

  Rosalba Perrone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... CD68-AF700 (BioLegend, clone FA-11, 137025, 1:200); IgM-APC-Cy7 (BioLegend ...
• PKC modulator bryostatin-1 therapeutically targets CNS innate immunity to attenuate neuroinflammation and promote remyelination

  Payam Gharibani, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-CD45 BV605 (1:400; clone 30-11; Biolegend), anti-Ly6G (1:400 ...
• Mitigating inflammatory bone loss in post-menopausal osteoporosis via targeting the IL-9 producing osteoclastogenic Th9 cells

  Leena Sapra, et al., bioRxiv - Immunology 2023

  Quote: ... cells were stimulated for the following 5h using a combination of protein transport inhibitor (Monensin) (Biolegend, USA), Ionomycin (1 ug/ml) ...
• The DNA damage response and RNA Polymerase II regulator Def1 has posttranscriptional functions in the cytoplasm

  O.T. Akinniyi, et al., bioRxiv - Molecular Biology 2024

  Quote: ... (HA.11, Biolegend). Signals were detected by fluorescence or chemiluminescence (ECL ...
• Local delivery of cell surface-targeted immunocytokines programs systemic anti-tumor immunity

  Luciano Santollani, et al., bioRxiv - Immunology 2024

  Quote: ... Granzyme B (QA16A02, BioLegend, 1:200). Intracellular staining was performed overnight at 4C ...
• BARD1 links histone H2A Lysine-15 ubiquitination to initiation of BRCA1-dependent homologous recombination

  Jordan R. Becker, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and mouse anti-HA (1:2000, HA.11 901501 Biolegend). Following primary ...
• Extrafollicular responses are sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus

  Lasse F. Voss, et al., bioRxiv - Immunology 2022

  Quote: ... IgD-AF488 clone 11-26c.2a (405718, BioLegend, 1:500), Ki67-eflour660 clone SolA15 (50-5698-82 ...
• Extrafollicular responses are sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus

  Lasse F. Voss, et al., bioRxiv - Immunology 2022

  Quote: ... IgD-AF488 clone 11-26c.2a (405718, BioLegend, 1:500), IgMb-BV510 clone AF6-78 (742344 ...
• CD38 regulates ovarian function and fecundity via NAD⁺ metabolism

  Rosalba Perrone, et al., bioRxiv - Developmental Biology 2023

  Quote: ... IgD-Alexa_Fluor_647 (BioLegend, clone 11-26c.2a, 405707, 1:800), CD68-AF700 (BioLegend ...
• Engineered display of ganglioside-sugars on protein elicits a clonally and structurally constrained B cell response

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgD-AF700 (1:200, 11-26c.2a, Biolegend), anti-mouse IgM-PE/Cy7 (1:200 ...
• Haptenic adducts of β-lactam antibiotics elicit antibody responses with narrow clonality and specificity

  Lachlan P. Deimel, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IgD-AF700 (1:200, 11-26c.2a, Biolegend), anti-mouse IgM-PE/Cy7 (1:200 ...
• Correction of age-associated defects in dendritic cell functions enables CD4⁺ T cells to eradicate tumors in the elderly

  Dania Zhivaki, et al., bioRxiv - Immunology 2022

  Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
• Prevalence and mechanisms of evolutionary contingency in human influenza H3N2 neuraminidase

  Ruipeng Lei, et al., bioRxiv - Microbiology 2022

  Quote: ... Cells were incubated with 1 μg mL-1 PE-anti HA.11 (BioLegend) at 4 °C with rocking for 1 h ...
• Donor Macrophages Modulate Rejection after Heart Transplantation

  BJ Kopecky, et al., bioRxiv - Immunology 2021

  Quote: ... anti-mouse CD68 1:200 clone FA-11 (Biolegend Cat# 137002), anti-Ki-67 1:200 (Invitrogen Cat# 14-5698-82 ...
• PTEN Inhibition Ameliorates Muscle Degeneration and Improves Muscle Function in a Mouse Model of Duchenne Muscular Dystrophy

  Feng Yue, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Alexa Fluor 647 anti-mouse CD68 (FA-11, BioLegend, 1:1,000), or anti-mouse MG53 (gift from Jianjie Ma ...
• Muscle Van Gogh-like 2 shapes the neuromuscular synapse by regulating MuSK signaling activity

  Myriam Boëx, et al., bioRxiv - Neuroscience 2020

  Quote: ... anti-HA.11 (1:100 for immunoprecipitation, Biolegend, catalog no. 902301), anti-HA (1:2500 for Western blotting ...
• Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies

  Ariel K. Frame, et al., bioRxiv - Neuroscience 2022

  Quote: ... and mouse anti-HA.11 epitope tag (901513, BioLegend; 1:1000). Subsequently ...
• Opposing diet, microbiome and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host

  Gabriel Vasconcelos Pereira, et al., bioRxiv - Microbiology 2023

  Quote: ... PerCP-Cy5.5-conjugated anti-IgD (Biolegend, 11-26c.2a; 1/200), PE-conjugated anti-IgE (Biolegend ...
• Inhibition of CD226 Co-Stimulation Suppresses Diabetes Development in the NOD Mouse by Augmenting Tregs and Diminishing Effector T Cell Function

  Matthew E. Brown, et al., bioRxiv - Immunology 2024

  Quote: ... and plate-bound 11-CD28 (BioLegend; RRID: AB_11147170; 1 µg/mL) or plate-bound 11-CD3 (BioLegend ...
• Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing

  Irene Carrozzo, et al., bioRxiv - Bioengineering 2024

  Quote: Cells were stained with HA.11 antibody (#901515, Biolegend 1:1000) at 4°C for 45 minutes ...
• Single-Cell Profiling of the Antigen-Specific Response to BNT162b2 SARS-CoV-2 RNA Vaccine

  Kevin J. Kramer, et al., bioRxiv - Immunology 2021

  Quote: ... granzyme b FITC (Biolegend 515403, 1:100), phospho-S6 APC Ser235/236 (Invitrogen 17-9007-42 ...
• Polymorphic centromere locations in the pathogenic yeast Candida parapsilosis

  Mihaela Ola, et al., bioRxiv - Genomics 2020

  Quote: ... Anti-HA.11 (BioLegend), at a 1:1000 dilution in milk/TBS blocking buffer (5 g non-fat dry milk to 100 ml TBS - 100 mM Tris–HCl ...
• Engineered Wnt7a ligands rescue blood brain barrier and neurobehavioral deficits in a mouse model of COVID-19

  Troy N. Trevino, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD68 (Biolegend FA-11), and Iba1 (Abcam ab178847) ...
• Influence of the Glymphatic System on α-Synuclein Propagation: Role of Aquaporin-4 and the Dystrophin-Associated Protein Complex

  Douglas M Lopes, et al., bioRxiv - Neuroscience 2024

  Quote: ... HA (Biolegend, HA.11). Following incubation with respective HRP-conjugated secondary antibodies ...