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• Ubiquitin-specific protease 8 controls B cell proteostasis and cell survival in multiple myeloma

  Almut Dufner, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and anti-mouse CD40 antibody (5 µg ml-1; HM40-3, Biolegend), or anti-IgM F(ab’)₂ fragment (5 µg ml-1 ...
• Spermidine/spermine N1-acetyltransferase controls tissue-specific regulatory T cell function in chronic inflammation

  Teresa Neuwirth, et al., bioRxiv - Immunology 2024

  Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
• The OSUMMER lines: a series of ultraviolet-accelerated NRAS-mutant mouse melanoma cell lines syngeneic to C57BL/6

  Brandon M. Murphy, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Lag-3 (Biolegend #C9B7W; 1:250), CD3 (BD Biosciences #145-2C11 ...
• Expression of Tim-3 drives naïve Treg to an effector-like state with enhanced suppressive activity

  Hridesh Banerjee, et al., bioRxiv - Immunology 2020

  Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ...
• Disruption of augmin-mediated microtubule nucleation in neural stem cells causes p53-dependent apoptosis and aborts brain development

  Ricardo Viais, et al., bioRxiv - Cell Biology 2020

  Quote: ... β3-tubulin (Biolegend #801201, dilution 1:1000), cleaved caspase-3 (Novus Biologicals #MAB835 ...
• Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

  Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Galectin-3 (1:1000; Biolegend, 125401), anti-γ-H2AX (1:200 ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... LAG-3-PE-Cy7 (1:200) (all Biolegend) and TCRβ-BV786 (1:200 ...
• Lipid-polymer hybrid nanoparticles utilize B cells and dendritic cells to elicit distinct antigen-specific CD4⁺ and CD8⁺ T cell responses

  Michael H. Zhang, et al., bioRxiv - Bioengineering 2022

  Quote: ... and 5 μg/mL of CD40 antibody ligand (clone: HM40-3; BioLegend).
• Heparan sulfate regulates amphiregulin signaling towards reparative lung mesenchymal cells during influenza A infection

  Lucas F. Loffredo, et al., bioRxiv - Immunology 2024

  Quote: ... rmIL-3 (Biolegend) was added to media (1 ng/ml) ...
• Retroviral Infection of Human Neurospheres and Use of Stem Cell EVs to Repair Cellular Damage

  Heather Branscome, et al., bioRxiv - Cell Biology 2021

  Quote: ... α-Tubulin β 3 (Tuj1; Biolegend MMS-435P; 1:200). The appropriate secondary antibody (Alexa Fluor 594 goat anti-rabbit ...
• STING suppresses cancer pain via immune and neuronal modulation

  Kaiyuan Wang, et al., bioRxiv - Cancer Biology 2021

  Quote: ... CD-3 APC/cy7 (1:200, rat, Biolegend, 100221), CD-4 APC (1:200 ...
• Prox2+ and Runx3+ neurons regulate esophageal motility

  Elijah D. Lowenstein, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit anti-Tubulin β-3 (BioLegend, Poly18020, 1:1000), goat anti-CTb (List Labs ...
• Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

  Luyao Ao, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Panel 3: BV421-conjugated CD45 (1:100, 103133, Biolegend), PE/Cy7 conjugated F4/80 (1:100 ...
• Naïve and in vitro-activated primary mouse CD8⁺ T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering

  Petra Pfenninger, Laura Yerly, Jun Abe, bioRxiv - Immunology 2021

  Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
• Molecular and functional diversity of distinct subpopulations of extracellular vesicles from stressed pancreatic beta cells: implications for autoimmunity

  Khem Raj Giri, et al., bioRxiv - Immunology 2020

  Quote: ... CD40 (3/23, BioLegend), CD115 (CSF-1R ...
• Complementary CRISPR screen highlights the contrasting role of membrane-bound and soluble ICAM-1 in regulating antigen specific tumor cell killing by cytotoxic T cells

  Ann-Kathrin Mattes, et al., bioRxiv - Cancer Biology 2022

  Quote: ... LAG-3 (BioLegend, 369212) or LFA-1 (BD ...
• Inulin prebiotic reinforces host cancer immunosurveillance via γδ T cell activation

  Emilie Boucher, et al., bioRxiv - Immunology 2022

  Quote: ... CD40 (3/23) (Biolegend), CD11b (M1/70) ...
• Adaptive IRE1 Signaling Elicits T Cell Metabolic Remodeling and Tumor Control

  Brian P. Riesenberg, et al., bioRxiv - Immunology 2023

  Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
• Bleomycin induced EBV-specific gastric epithelial cell death is enhanced by LMP2A

  Aung Phyo Wai, et al., bioRxiv - Microbiology 2024

  Quote: ... caspase-3 (W20054B; Biolegend), cleaved caspase-3 (Asp175 ...
• Acetyl-CoA Carboxylase Obstructs CD8⁺ T-Cell Lipid Utilization and Energy Synthesis in the Tumor Microenvironment

  Elizabeth G. Hunt, et al., bioRxiv - Immunology 2023

  Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
• HSV-1 Oncolytic Virus Targeting CEACAM6-Expressing Tumors Using a Bispecific T-Cell Engager

  Yanal M. Murad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Tim-3 (BV510, BioLegend), LAG-3 (BV605) ...
• Lesion-specific suppression of YAP/TAZ by biomimetic nanodrug ameliorates atherosclerosis development

  Hui-Chun Huang, et al., bioRxiv - Bioengineering 2023

  Quote: ... Histone 3 (BioLegend #819411), YAP/TAZ (Santa Cruz #sc-101199) ...
• Multi-Omic Atlas reveals cytotoxic phenotype and ROS-linked metabolic quiescence as key features of CTL-resistant HIV-infected CD4⁺ T-cells

  Alberto Herrera, et al., bioRxiv - Immunology 2024

  Quote: ... #3 and #4 (Biolegend) were spiked into individual samples ...
• Cortical hyperexcitability drives dying forward ALS symptoms and pathology in mice

  Mouna Haidar, et al., bioRxiv - Neuroscience 2021

  Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
• Excess BAFF Alters NR4As Expression Levels and Breg Function of Human Precursor-like Marginal Zone B-cells in the Context of HIV-1 infection

  Kim Doyon-Laliberté, et al., bioRxiv - Immunology 2022

  Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
• Analysis of the Diverse Antigenic Landscape of the Malaria Invasion Protein RH5 Identifies a Potent Vaccine-Induced Human Public Antibody Clonotype

  Jordan R. Barrett, et al., bioRxiv - Immunology 2023

  Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
• Molecular Imaging with Fibroblast Activation Protein Tracers depicts Inflammatory Joint Damage and its Transition to Resolution of Inflammation

  Simon Rauber, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
• Activity-dependent development of the body’s touch receptors

  Celine Santiago, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit anti-Tubulin beta 3 (TUBB3) (Biolegend #Poly18020, 1:500), rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP ...
• Interleukin-1 receptor antagonist is a conserved driver of tuberculosis

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2024

  Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (1-3; BioLegend). The stained T cell samples were then incubated with Sytox Green Dead Cell Stain (Thermo Fisher Scientific ...
• Selective suppression of oligodendrocyte-derived amyloid beta rescues neuronal dysfunction in Alzheimer’s Disease

  Rikesh M. Rajani, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-Tubulin beta 3 (clone TUJ1; 1:1,000; BioLegend; 801202), IBA1 (1:500 ...
• Stratified analysis identifies HIF-2α as a therapeutic target for highly immune-infiltrated melanomas

  Amy Y. Huang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... TIM-3 BV711 (clone RMT3-23, Biolegend 119727, 1:200), Foxp3 FITC (clone FJK-16s ...
• Dietary Protection Against the Visual and Motor Deficits Induced by Experimental Autoimmune Encephalomyelitis

  Katarzyna Zyla-Jackson, et al., bioRxiv - Immunology 2023

  Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
• An adapted stem cell-derived microglia protocol for the study of microgliopathies and other neurological disorders

  Marie-France Dorion, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-CX-3-C motif chemokine receptor 1 (CX3CR1; clone #2A9-1, Biolegend), anti-Mer tyrosine kinase (MERTK ...
• Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons

  Ching-Chieh Chou, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197 & 1:100; Biolegend, 125401), anti-Hsp70 (1:300 ...
• Proteome and functional decline as platelets age in the circulation

  Harriet E. Allan, et al., bioRxiv - Cell Biology 2021

  Quote: Platelet subpopulations (3 × 106) stained with CD42b-BV421 (1:300; Biolegend) in (i ...
• MR1-restricted MAIT cells from the human lung mucosal surface have distinct phenotypic, functional, and transcriptomic features that are preserved in HIV infection

  Sharon Khuzwayo, et al., bioRxiv - Immunology 2020

  Quote: ... and 1:50 of anti-TIM-3 BV785 (F38-2E2, BioLegend). Tetramer staining was performed in the dark at room temperature for 40 minutes ...
• Effective NGN2-based neuronal programming of hiPSCs in an automated microfluidic platform

  S Angiolillo, S Micheli, O Gagliano, bioRxiv - Neuroscience 2022

  Quote: ... and mouse anti-TUBULIN β 3 (βIIITUBB) (1:5000, 801202 BioLegend) and MAP2 (1:10000 ...
• Noise-induced changes in rat head stability and orientation correlate with dysfunction of otolith irregular afferents

  Mamiko Niwa, et al., bioRxiv - Neuroscience 2023

  Quote: ... or rabbit anti-tubulin beta 3 (Tubb3, 1:1000, BioLegend Poly18020), and rabbit anti-myosin 7a (Myo7a ...
• Slow kinesin-dependent microtubular transport facilitates ribbon synapse assembly in developing cochlear inner hair cells

  Roos Anouk Voorn, et al., bioRxiv - Neuroscience 2024

  Quote: ... and anti-β-3-tubulin (Tuj-1; Mouse IgG2a, BioLegend, #801202) and a fluorescently-conjugated nanobody directed against PSD-95 (Fluo-Tag-X2 ...
• A brain-shuttled antibody targeting alpha synuclein aggregates for the treatment of synucleinopathies

  Sungwon An, et al., bioRxiv - Neuroscience 2024

  Quote: ... mouse anti-Tubulin β 3 (TUBB3)-AF647 1:300 (BioLegend 801210) overnight and counter stained with DAPI ...
• Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling

  Eugene Joeh, et al., bioRxiv - Biochemistry 2020

  Quote: ... Recombinant galectin-3 (Biolegend # 599706) was then incubated either for 1-2 hrs at RT or overnight at 4°C ...
• Cross-Linking of T cell to B cell lymphoma by the T cell bispecific antibody CD20-TCB induces IFNγ/CXCL10-dependent peripheral T cell recruitment in humanized murine model

  Floriana Cremasco, et al., bioRxiv - Immunology 2020

  Quote: ... 3) CD62L APC-Cy7 (BioLegend), huCD45 PE (BioLegend) ...
• A method for polyclonal antigen-specific T cell-targeted genome editing (TarGET) for adoptive cell therapy applications

  Darya Palianina, et al., bioRxiv - Immunology 2022

  Quote: ... LAG-3-BV711 (11C3C65, Biolegend) and TIGIT-BV605 (A15153G ...
• Potent Neutralizing Antibodies Directed to Multiple Epitopes on SARS-CoV-2 Spike

  Lihong Liu, et al., bioRxiv - Microbiology 2020

  Quote: ... and human Hashtag 3 (Biolegend) at 4°C for 1 hr ...
• Tumor suppressor p53 deficiency increases tumor immunogenicity through promoting IL33-mediated anti-tumor immune responses

  Yang Li, et al., bioRxiv - Immunology 2022

  Quote: ... LAG-3 (BioLegend, 125212, C9B7W), ST2 (eBioscience ...
• Distinct effects of disease-associated TREM2 R47H/+ and T66M mutations on iPSC-derived microglia

  Jay Penney, et al., bioRxiv - Neuroscience 2022

  Quote: ... rabbit-β-Tubulin 3 (BioLegend) and mouse-STEM101 (Takara Bio) ...
• Enhancing Mucosal-Associated Invariant T (MAIT) cell function and expansion with human selective serum

  Daniel Labuz, et al., bioRxiv - Immunology 2022

  Quote: ... anti-TIM-3-BV421 (Biolegend), and anti-human MR1-5-OP-RU Tetramer (NIH Tetramer Core Facility) ...
• Enhancing Mucosal-Associated Invariant T (MAIT) cell function and expansion with human selective serum

  Daniel Labuz, et al., bioRxiv - Immunology 2022

  Quote: ... anti-LAG-3-BV786 (Biolegend), anti-CD69-BUV563 (BD Biosciences) ...
• Enhancing Mucosal-Associated Invariant T (MAIT) cell function and expansion with human selective serum

  Daniel Labuz, et al., bioRxiv - Immunology 2022

  Quote: ... anti-LAG-3-BV786 (Biolegend), anti-CD69-APC (Biolegend) ...
• Enhancing Mucosal-Associated Invariant T (MAIT) cell function and expansion with human selective serum

  Daniel Labuz, et al., bioRxiv - Immunology 2022

  Quote: ... anti-LAG-3-BV786 (Biolegend), anti-CD25-BV650 (Biolegend) ...