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Citations for BioLegend :
4751 - 4800 of 10000+ citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The phenotype of differentiated HL-60 cells was assessed by flow cytometry using mouse antihuman CD35 PE conjugated antibody (BioLegend) and mouse anti-human CD71 APC conjugated antibody (BioLegend) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were assessed for titers of different antibody isotypes using LegendPlex Mouse Immunoglobulin Isotyping Panel and IgE ELISA (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... 0.1−0.5×106 cells were incubated for 15 min at 4 °C with anti-mouse Fc-block CD16/32 antibody (clone 93, 1:100) and True-Stain Monocyte Blocker (1:100) (both from Biolegend), in 100 µl FACS staining buffer (1.5% FBS and 0.5% BSA in PBS) ...
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bioRxiv - Immunology 2024Quote: ... Cells from individual skin tissue samples were simultaneously stained with corresponding mouse TotalSeq-B hashtag index antibodies (B0251-B0260; Biolegend), FACS antibody cocktail (CD45 ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Avidin-HRP (100 μl, cat. #405103, Biolegend, San Diego, CA, USA) was diluted 500 times in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated streptavidin (BioLegend) for PLA2G12A or HRP-conjugated sheep anti-mouse IgG F(ab’)2 fragment (Amersham ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated with fluorescently-labelled antibodies in the presence of anti-CD16/CD32 Fc blocking antibody (2.5 μg/mL, Biolegend, San Diego, CA, USA) for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... we cultured mouse T cells in mouse RP10 with recombinant mouse TGF-β1 (Biolegend Cat # 763104), rIL-12 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... with 1 × 106 cells/mL 5000 rad irradiated PBMC + 50 U/mL IL-2 + 10 ng/mL IL-15 (both from NCI BRB Preclinical Biologics Repository) + 0.1 μg/mL each of anti-CD3 (Ultra-LEAF purified anti-human CD3 antibody clone OKT3; BioLegend, San Diego, CA) and anti-CD28 (Ultra-LEAF purified Anti-human CD28 antibody clone 28.2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309, anti-HA Alexa 647, Biolegend, cat #682404). Cells were washed in PBS 0.5% BSA (0.5% BSA ...
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bioRxiv - Pathology 2024Quote: ... then incubated with primary antibody anti-IBA1 (Wako; 011-27991; dilution 1:100) and anti-Amyloid Beta1-42 (Biolegend; 805501; 1:500) prepared in 1% BSA for overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: TruStain FcX™ PLUS antibody was used to block samples then biotin anti-lineage antibodes and biotin anti-Flk1 (Biolegend, clone 89B3A5) antibody were used to stain cells ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for purity: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD20 PerCP/Cy5.5 (BioLegend catalog #375510 ...
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bioRxiv - Immunology 2024Quote: ... Plates were coated with 3µg/ml anti-CD3 and 3 µg/ml of anti-CD28 antibody (Biolegend clones 145-2C11 and 37.51) for 2 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). After enrichment for S1 ...
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bioRxiv - Immunology 2021Quote: ... The PBMC samples were stained with 5 µl of anti-mouse CD3e-PerCP/cy5.5 (Biolegend, USA, cat#100327), NK1.1-PE (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...
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bioRxiv - Biochemistry 2020Quote: ... and then stained with 10 μg/mL AlexaFluor647 Armenian hamster anti-mouse CD3ε (clone 145-2C11, BioLegend, USA) followed by 0.5 μg/mL 7-AAD (AAT Bioquest) ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)) ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) before fixation ...
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bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)) ...
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bioRxiv - Physiology 2022Quote: ... Pellet was resuspended in 100μL of sorting buffer and 10μL of MojoSort Mouse anti-APC Nanobeads (BioLegend, 480072) was added ...
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bioRxiv - Immunology 2022Quote: ... BioLegend) for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2 ...
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bioRxiv - Biochemistry 2022Quote: ... and stained for flow cytometry using the neutrophil marker Ly6G (PE-anti-Mouse Ly6G; Cat No. 127608; BioLegend), gating for side-scatter and PE+ cells.
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bioRxiv - Immunology 2020Quote: Saporin-linked ADCs targeting murine CD45.2 (CD45-SAP) and cKit (cKit-SAP) were generated by incubating biotinylated anti-mouse CD45.2 (clone 104, BioLegend) or biotinylated anti-mouse cKit (clone 2B8 ...
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bioRxiv - Cancer Biology 2022Quote: ... and mHTO05 TotalSeqA from BioLegend Staining was performed as per the manufacturer’s guidelines using TruStain FcX™ PLUS (anti-mouse CD16/32) (BioLegend) as a blocking agent ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with a mix of Fc Block (anti-mouse CD16/CD32 mAb; clone 2.4G2, BioLegend) and fluorophore-conjugated anti-mouse mAbs.
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bioRxiv - Immunology 2022Quote: ... 5 min prior to euthanasia each mouse was injected retro-orbitally with 5ug of anti-CD45.2-APC (Biolegend) diluted in 200 ul of PBS.
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bioRxiv - Immunology 2021Quote: ... and CD3+ cells were depleted from the donor cells using a biotinylated anti-mouse CD3e mAb (BioLegend; 100304) and the Miltenyi MACS® MicroBead (Miltenyi ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted and washed with PBS before staining with TruStain FcX anti-mouse CD16/32 (Biolegend 101319) or TruStain FcX anti-human (Biolegend 422301 ...
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bioRxiv - Immunology 2023Quote: ... and ST2 were determined by flow cytometric analyses using FITC-labeled anti-mouse FcεRIα (clone MAR-1, BioLegend), APC-labeled anti-mouse CD117 (clone 2B8 ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were quantified by a fluorescence-encoded bead-based multiplex assay (LEGENDplex Mouse Anti-Virus Response Panel, BioLegend), per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
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bioRxiv - Immunology 2024Quote: ... all cells were blocked with 1:50 dilution of TruStain FcX anti-mouse CD16/CD32 (Clone 93, Biolegend) and Fixable Viability Dye (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... TOYOBO) supplemented with 50 μg/mL of FITC anti-mouse I-A/I-E (clone M5/114.15.2, BioLegend) for 1 h at room temperature in the dark following pretreatment with Ultra-LEAF purified anti-mouse CD16/32 Ab (clone 93 ...
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bioRxiv - Immunology 2024Quote: NK cells were defined as positive for CD56dim using Brilliant Violet 650 conjugated mouse anti-human CD56 (BioLegend) and PerCP/Cyanine5.5 conjugated mouse anti-human CD16 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD279 (PD-1) (clone: RMP1-30, PE, Biolegend, cat# 109104; clone: RMP1-30, PE-Cy7, Biolegend, cat# 109110 ...
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bioRxiv - Cell Biology 2022Quote: ... CA) and phycoerythrin/Cy7 (PECy7)-conjugated anti-mouse CD71 (clone RI7217; 5 μg/mL; BioLegend, San Diego, CA) antibodies were added ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated pancreatic cells/units were incubated with anti-mouse CD16/32 (10 μg/mL; BioLegend, San Diego, CA) for 5 min on ice to reduce non-specific binding ...
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bioRxiv - Bioengineering 2022Quote: ... and then 1:200 dilution of Alexa Fluor 488 mouse anti-rat CD45 (BioLegend, San Diego, CA, USA), PE mouse anti-rat CD31 (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated for 10 minutes with purified rat anti-mouse CD16/CD32 (Biolegend, 93 clone)(only for mouse cells ...
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bioRxiv - Immunology 2022Quote: ... Heart CD45+ cells were enriched via positive selection using 0.2μg anti-mouse CD45 PE (Biolegend, clone 30-F11) per 1×106 cells followed by anti-PE microbeads (Biolegend) ...
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bioRxiv - Neuroscience 2024Quote: ... anti-PAX6 (1:250, mouse monoclonal, MA1-109 Thermo Fisher Scientific or 1/500, rabbit polyclonal, 901301 Biolegend); anti-TBR2 (1:500 ...