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Citations for BioLegend :
4701 - 4750 of 6837 citations for TGFB3 Human Mouse Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and a 1:12,000 dilution of mouse monoclonal α-RpoA (BioLegend) primary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell suspensions were incubated with mouse Fc Block (BD or BioLegend). B cells were isolated from Spleen using biotinylated anti-CD19 antibody (BD Pharmingen ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used: Alexa Fluor® 488 anti-human CD45 antibody (clone H130, catalog #304019, BioLegend); APC anti-human CD3 antibody (clone HIT3a ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dilutions were used: anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... B cells were washed with FACS buffer and stained with PerCP-cy5.5 anti-human CD19 antibody (Biolegend, 363016), FITC anti-human CD3 antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: IL-21 concentration from tonsil mono-cultures was measured using Human IL-21 ELISA MAX Deluxe kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... Samples were thawed on ice prior to detection and analysis using LEGENDplex human hematopoietic stem cell panel (BioLegend), according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used for flow cytometry staining: APC/Cy7 anti-human CD3 Antibody (clone HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and the production of IFN-γ and Granzyme B was checked with PerCP anti-human IFN-γ (BioLegend) and FITC anti-human Granzyme B (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were stained for 30 minutes with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with an anti-human CD271(ΔNGFR) PE-conjugated antibody (BioLegend, Cat. No. 345106, RRID: AB_2152647) (dilution 1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the purity of the monocyte/macrophage population was checked by staining with FITC anti-human CD14 antibody (BioLegend) and FC and used for infection.
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bioRxiv - Cancer Biology 2022Quote: The cells used in this study were labeled with anti-human B7-H3 monoclonal antibody (Biolegend, clone MIH42) PerCP/Cyanine 5.5 conjugate at 4°C for 45 minutes in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked in 1X PBS containing 1 mg/ml BSA and 2.5 µl of human FcX-TruStain (BioLegend Inc.) for 15 minutes and incubated with PE-c-CASPASE3 antibody (Cell Signaling #9978 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... HIV-infected cells were previously incubated with 10 μg/ml of anti-human HLA-E (clone 3D12, Biolegend) antibody for 20 mins at RT ...
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bioRxiv - Microbiology 2020Quote: ... the purity of the monocyte/macrophage population was checked by staining with FITC anti-human CD14 antibody (BioLegend) and FC ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Human trachea was stained for 72 hours in PBS serum and the following antibodies: hCD103-AlexaFluor 594 (Biolegend) 1:40 ...
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bioRxiv - Cancer Biology 2021Quote: ... The stained samples were then incubated and stained with Alexa Fluor® 700 anti-human CD45 Antibody (BioLegend). Post staining ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Sections were stained with the primary antibodies: anti-human CD105 (endoglin, ENG) biotin-conjugated (1:100, BioLegend, 323214), anti-human CD31-Alexa Fluor® 594 conjugated (1:400 ...
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bioRxiv - Immunology 2019Quote: ... Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend) and data acquired on a BD Symphony A5 cytometer ...
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bioRxiv - Immunology 2019Quote: The following fluorescent conjugated antibodies were used: antibodies to human CD3ε-BUV395 or PeCy7 (UCHT1; eBioscience and Biolegend), CD4-PerCPCy5.5 (RPA-T4) ...
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bioRxiv - Immunology 2019Quote: ... Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1, BioLegend), BV605-conjugated anti-human CD4 (OKT-4 ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Immunology 2019Quote: ... Human MAIT cells were isolated by sorting CD2 MACS-enriched (Miltenyi) leukocytes with CD161 and Vα7.2 antibodies (BioLegend). MAIT cells were grown for around 6 weeks in complete RPMI media supplemented with IL-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... resuspended in FACS buffer (1X PBS containing 0.5% FBS and 0.01% sodium azide) and used for staining with the following anti-human antibodies (Biolegend): CD45 ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...
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bioRxiv - Immunology 2021Quote: ... cells were also stained with Alexa Fluor 488 anti-human TCR α/β (306712, BioLegend, San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... Murine monoclonal FITC anti-human CD11b (activated) antibody (IgG1, clone CBRM1/5) was from Biolegend (San Diego, CA). Goat anti-mouse (GαM ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... permeabilized for 10 min in PBS/0.1% saponin and stained with FITC-conjugated anti-human Perforin (γG9, Biolegend) and PE-conjugated anti-human Perforin antibody (B-D 48 ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Cancer Biology 2022Quote: ... Blocking buffer was then removed and cells were incubated with an APC-conjugated anti-human EpCAM (BioLegend, 369809) antibody diluted 1:20 in dilution buffer (1x PBS-T ...
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bioRxiv - Bioengineering 2022Quote: ... Enriched CD34+ HSPCs were stained with APC anti-human CD34 antibodies (clone 581, Biolegend, San Jose, CA, USA), and sample purity was assessed on an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with antibodies directly conjugated to fluorescent dyes (anti-human BAFF-R conjugated to FITC, BioLegend, Cat ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the extracellular staining according to the manufacturer’s protocol: PerCP/Cyanine5.5 anti-human CD3 Antibody (#300430, BioLegend), Brilliant Violet 421 anti-human CD4 Antibody (#357424 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110 ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Genomics 2024Quote: ... incubated with 0.5ug of a unique Cell Hashing antibody (TotalseqTM-A0251 to A0257 anti-human hashtag antibody, BioLegend) for 20 minutes at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in saliva were measured twice per sample using the LEGENDplex Human Essential Immune Response panel (BioLegend), as described in previous studies 26,57 ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...