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Citations for BioLegend :
4701 - 4750 of 7947 citations for Rat WIF 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the femurs of mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Cancer Biology 2024Quote: ... and then labeled with Carboxyfluorescein Succinimidyl Ester (CFSE) using CFSE Cell Division Tracker Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice in PBS and stained with Zombie UV Fixable Viability Kit (Biolegend) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... granulocyte and macrophage (1:1000, PECy7-Gr-1/clone RB6-8C5 and PECy7-CD11b /clone M1/70, Biolegend Cat.108416 and Cat.101216), then analyzed by flow cytometry (BD MoFlo XDP).
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bioRxiv - Immunology 2021Quote: ... cells were stained in PBS at 4°C for 30 minutes with the fixable viability dye eFluor 780 (1:1000, 65-0865-14 eBioscience) and a combination of the following antibodies (1:200, all from BioLegend unless stated otherwise): anti-mouse CD45 BV650 (30-F11 ...
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bioRxiv - Microbiology 2019Quote: ... Latently infected CD14+ monocytes were fixed with 1% paraformaldehyde and stained using anti-HLA-DR Pacific blue conjugate (Biolegend, Clone L243, 1:50) and anti-HLA-A,B,C ...
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bioRxiv - Genomics 2020Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...
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bioRxiv - Immunology 2019Quote: ... After isolation cells were treated with Fc receptor blocking solution and stained with anti-ICAM-1-PE (1 μg/ml, HA58; Biolegend, San Diego, USA), anti-VCAM-1-BV711 (0.5 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 100 µl containing 1 × 106 cells in PBS were incubated with yellow Zombie viability dye (1:1000 dilution, Cat. No 423104, Biolegend, San Diego, CA) for 15 minutes at room temperature followed by washing in FACS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Cell Biology 2020Quote: ... after blocking cells were incubated with 1:250 dilution of rabbit anti-TbKH pAb and 1:500 dilution of mouse anti-HA mAb (BioLegend, Cat# MMS-101R). As a negative control ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Immunology 2022Quote: ... CD11bhi, F4/80hi) were isolated by FACS using antibodies against CD11b (eBioscience, #11-0112, 1:1600) and F4/80 (Biolegend, #123114, 1:200), and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... or biotinylated anti-repebody antibody (1 μg/mL, AbClon, Seoul, Korea) and HRP-conjugated streptavidin (1:1000 dilution, BioLegend, San Diego, CA, USA). For trastuzumab (Herceptin) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
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bioRxiv - Immunology 2021Quote: ... followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). After enrichment for S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated at 4°C overnight with rabbit sera against MmuPV1 L1 (a kind gift form Chris Buck, NIH; 1:2500) and chicken anti-keratin 14 (BioLegend 905301; 1:500) diluted in 5% goat serum in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were suspended in 300 μL staining buffer and incubated on ice for 30min with FVD780(eBioscience,1:1000) and then with CD45(Biolegend,202205, 1:300) for 30min ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... For the exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) was adjoined and incubated for 30 min dRT ...
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bioRxiv - Immunology 2021Quote: ... and for exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in FACS buffer and stained using a Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were purified using negative magnetic bead selection (MojoSort Mouse Pan Dendritic Cell Isolation Kit, BioLegend). 2.5×106 BMDCs per well were cultured for 6h in 6-well plates in RPMI complete (R10 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCT-treated CD11b+ cells were isolated from tumors using MojoSort Mouse CD11b Selection Kit (BioLegend, 480109). For transfecting every million primary CD11b+ cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Apoptosis and necrosis were determined by using the FITC Annexin V Apoptosis Detection Kit (BioLegend 640914) and SYTOX™ Red Dead Cell Stain (ThermoFisher S34859) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... splenocytes were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (BioLegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cells were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Microbiology 2022Quote: Serum IFN-γ was assessed using a custom-made mouse panel 13-plex kit (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... the labeled cells were washed and incubated with Zombie UV™ Fixable Viability Kit (BioLegend #423107) in FACS buffer before analysis ...
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bioRxiv - Cell Biology 2019Quote: Apoptosis in mononuclear cell suspensions was assessed using the Annexin V-FITC Apoptosis Kit (BioLegend, 640914) according to manufacturer’s instructions and analyzed via flow cytometry ...
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bioRxiv - Immunology 2019Quote: ... FSC-A scatter plots) and dead cells identified by using Zombie Aqua Fixable Viability Kit (BioLegend) were excluded from analyses ...
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bioRxiv - Immunology 2021Quote: ... with all incubations carried out in selection buffer using the MojoSort Mouse Ly6G Selection Kit (Biolegend), the EasySep Biotin Positive Selection Kit II (Stemcell Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and immediately processed with the Biolegend Phase-Flow Alexa Fluor 647 kit (BioLegend). Cells were counterstained with DAPI and at least 10 000 events were acquired on a BD Fortessa (Becton Dickinson).
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bioRxiv - Cancer Biology 2021Quote: ... Proliferation of CD8+ T cells was determined using the CFSE Cell Division Tracker Kit (Biolegend 423801) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2020Quote: Cell death was detected with the FITC Annexin V Apoptosis Detection kit (BioLegend, Cat No. 640922). Infections were performed as described above with mCherry-Stm at an MOI of 100 ...
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bioRxiv - Immunology 2021Quote: ... with half undergoing naïve CD4+ T cells isolation using MoJo magnetic bead negative selection kits (BioLegend), and the other half undergoing CD90+ cell depletion (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were assessed using the APC AnnexinV Apoptosis Detection Kit with PI (BioLegend, CA, USA; #640932) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The plate was finally washed eight times and was developed with the TMB substrate kit (Biolegend) according the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder of the cells were labelled using the Zombie Aqua Fixable Viability Kit (BioLegend #423101) according to the manufacturer’s instructions to indicate the viability status of each cell ...
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bioRxiv - Immunology 2024Quote: ... using the MojoSort magnet with MojoSort Mouse CD4 Naïve T Cell Isolation Kit (Cat.#480040 BioLegend). T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse ...
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bioRxiv - Cancer Biology 2024Quote: Annexin V / PI stainings were performed using the APC/FITC Annexin V Apoptosis Detection Kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were enriched from total splenocytes using the EasySep Mouse CD8+ T cell Isolation Kit (Biolegend) at day 3.5 post infection ...
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bioRxiv - Immunology 2023Quote: ... Airway cytokines and chemokines were quantified by multiplex analyses of BALF samples using LEGENDplexTM kits (BioLegend). Quantification of airway bioactive type I/III IFN was conducted by treating type I/III IFN-sensitive cells isolated from Mx2-Luc reporter mice for 24 h with diluted BALF ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 30 min at RT with Zombie Green™ Fixable Viability Kit (Biolegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 24h and 48h using an apoptosis detection kit with PE-Annexin V and 7-AAD (Biolegend), according to the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Sorted γδ T cells were stained with 2.5 nM CFSE Cell Division Tracker Kit (423801, BioLegend) for 20 min at 37 °C immediately after sorting ...