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Citations for BioLegend :
4701 - 4750 of 6202 citations for NADH Cytochrome B5 Reductase 2 CYB5R2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... and immunofluorescence staining for PECAM-1 (AlexaFluor488 labelled primary monoclonal rat antibody, 5μg mL-1, MEC13.3, BioLegend) and S100A9 (Cy5.5 directly labelled ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then stained with APC anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody (BioLegend, 1:260) for 20 min ...
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bioRxiv - Immunology 2024Quote: ... Samples were stained for surface antigen expression using indicated antibodies (Key Resources Table) and TotalSeq HashTags (BioLegend) for 30 minutes at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Naïve CD4 T cells were treated with fluorescein isothiocyanate-labeled (FITC) anti-mouse CD45RB antibody (BioLegend 103305), followed by washing ...
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bioRxiv - Pathology 2024Quote: ... Following antibodies were purchased and used for flow cytometry analysis: CD45 (30F-11, BD bioscience and Biolegend), CD11b (M1/70 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with a PE-conjugated mouse monoclonal antibody against CD82 (1:50 dilution; 342104, BioLegend) in FACS wash (dPBS with 5% fetal bovine serum (FBS)) ...
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bioRxiv - Immunology 2024Quote: ... The cells were then pooled and stained with fluorochrome-conjugated primary antibodies BV650-CD45.2 (BioLegend® 109835) and PerCP/Cy5.5-CD11b (BioLegend® 101228 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained on ice with the following antibodies (all 1:500): rat isotype PE (BioLegend 400507), CD80 PE (eBioscience 12-0801-82) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were incubated with monoclonal antibodies against F4/80 (BM8; Biolegend, San Diego, CA, USA; 1:600), CD8alpha (D4W2Z ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumor cells were labeled with Ep-CAM (APC/Cyanine7 anti-mouse CD326 Antibody, BioLegend, San Diego, CA) for identification ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies in 1:200 dilutions were used: CD31-PE-594 (1:200, Biolegend, Cat# 102520), CD45-APC (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with primary antibodies: anti-MBP (Biolegend 808401, 1:200, anti-pMLC2(S19/T18) (CST 3674 ...
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bioRxiv - Neuroscience 2024Quote: ... the iAstrocytes were stained with 1:50 PE anti-human HLA-A,B,C Antibody (BioLegend, 311406) and DAPI for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control, Pacific Blue Rat IgG2a, k; #400527, Biolegend). For GD3 FACS analysis ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were stained for 45 minutes with 1:1000 PE conjugated anti-HA.11 antibody (BioLegend, #901518 ...
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bioRxiv - Immunology 2024Quote: 3−5×107 PBMC were stained using a cocktail of antibodies that included CD3 (clone UCHT1, Biolegend), CD4 (clone OKT4 ...
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bioRxiv - Immunology 2024Quote: ... The blocked tissue sections were stained using primary antibodies: chicken anti-keratin 14 (Biolegend #906004, 1:1000), rabbit anti-citrullinated histone H3 (Abcam #5103 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with antibodies including Brilliant Violet 421-anti-B220 (RA3-6B2, Biolegend, San Diego CA), fluorescein isothiocyanate-anti-BP-1 (6C3 ...
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bioRxiv - Neuroscience 2024Quote: ... 75 and validated via Coomassie blue staining and western blot using the 6E10 antibody (1:1000; BioLegend).
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated 20-60 minutes at room temperature and continued by adding 2 mL of 1X Permeabilization Buffer (Biolegend Cat. No. 421002) and centrifuged at 400-600 x g for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Enzyme-linked immunosorbent assays were completed using and according to the instructions found with the ELISA MAX™ Deluxe Set Human IL-2 (BioLegend #431804).
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentrations in supernatant from mock and SARS- CoV-2 infected lung AT2 and cardiac cells were analyzed using the LEGENDplex human anti-virus response panel (Biolegend, Cat. 740390) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated for 30 min at 370C with 1:500 2-NBDG and 1:1000 live-dead stain Zombie NIR (Biolegend, cat.no. 423106) in cell culture media with either high or low insulin concentration ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Neuroscience 2020Quote: ... 50-μm brain sections were blocked with normal goat serum for 2 h and incubated overnight using biotin-conjugated anti-HA (Biolegend 901505, 1:500) and anti-parvalbumin (Synaptic Systems 195 004 ...
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bioRxiv - Neuroscience 2020Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...
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bioRxiv - Developmental Biology 2020Quote: ... centrifuged in a table top centrifuge at 1,500rpm for 5 minutes and resuspended in 200µl staining solution containing conjugated antibodies Ep-CAM-APC (#118213 Biolegend) and Brilliant violet 785-CD45 (#103149 Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... 10μg/mL CEA-Antibody (Novus BioScience #NBP2-34594-0.1 mg) or Isotype Control (mo IgG1 Isotype, BioLegend #401402) were added to the cells for 30minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit polyclonal antibody directed against Myosin-IIA (#909801, IF dilution 1:100) was from Biolegend (Princeton, NJ, USA). Mouse monoclonal antibody directed against α1-actinin (#TA500072S ...
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bioRxiv - Immunology 2021Quote: ... treated with DNase for 60minutes and then intracellularly stained for 30 minutes with APC anti-BrdU antibody (Biolegend).
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bioRxiv - Immunology 2020Quote: After 16 h stimulation the cells were washed and stained with anti-HLA A2 monoclonal antibody BB7.2 (Biolegend) for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dilutions were used: anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: TRA-1-60 staining was performed as previously described (4) and anti-SSEA1 antibody (Biolegend, catalog no. 125604) was used to quantify MEF reprogramming ...
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bioRxiv - Molecular Biology 2020Quote: ... The heme dose response was mixed 1:1 with 2X-concentrated antibodies conjugated to fluorescent dyes (FITC; Biolegend #100510 and eFluor780 ...