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Citations for BioLegend :
4601 - 4650 of 5368 citations for Mouse Relaxin 3 RLN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... T cells at 1×106/ml were kept in culture withdraw of cytokines for 8 hours before stimulated with 1µg/ml plate-coated anti-mouse CD3e (Biolegend, Cat.100340) and 1µg/ml soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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bioRxiv - Genetics 2024Quote: ... was collected in EDTA-coated capillary and was stained for 15 min in the dark at room temperature with APC labelled anti-mouse CD45.1 antibody (Biolegend, 1:20) and PE labelled anti-mouse CD45.2 antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Immunology 2024Quote: ... Cells were next stained for 30 mins on ice with a 1:300 dilution of antibodies raised against mouse CD206 (Biolegend,141710), CD301(Biorad ...
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bioRxiv - Immunology 2024Quote: ... The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel catalog #740493 with the V-bottom plate was used according to manufacturer’s instructions (BioLegend, CA). Briefly ...
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bioRxiv - Immunology 2024Quote: Cells were pelleted and then resuspended in staining buffer and stained for 30 minutes on ice with 1 mg/mL AF700-mouse-anti-human CD45 (BioLegend; 368508) and PE-mouse anti-human CD235a in staining buffer ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Maxpar Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) for FC blocking (10 min ...
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bioRxiv - Immunology 2023Quote: ... and labelled with anti-mouse TNF-ɑ (clone: Mab11, FITC), IFN-γ (clone: 4S.B3, BV-650) (BioLegend, San Diego, CA, USA), and GrzB (clone ...
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bioRxiv - Microbiology 2023Quote: Lung single-cell suspensions were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody (Cat: 101320; BioLegend, San Diego, CA) in eBioscience™ Flow Cytometry Staining Buffer (Cat ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... The samples were washed with Cell Staining Buffer and stained with anti-mouse TCR β chain-Alexa Fluor® 647 (Biolegend) at a concentration of 0.02μg/μl ...
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bioRxiv - Bioengineering 2023Quote: Secreted cytokine levels into the collected IKKβCA- and Control-CMs were measured using 9-Plex LEGENDPlex mouse inflammation panel (BioLegend, Cat #740446) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were generated by passing through 70 μm filters and sequentially stained with APC anti-mouse CD45 (Biolegend #103111) and propidium iodide (Sigma ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: The quantification of cytokines was performed with the LegendPlex mouse Th cytokine panel according to the protocol provided by the manufacturer (Biolegend, USA). The amounts of cytokine per cell were calculated as follows ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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bioRxiv - Developmental Biology 2023Quote: ... Then cells were again washed and followed by an incubation with anti-mouse IgG coupled to PE-Cy7 (Biolegend ref 406613) at a 1/200 dilution in BD wash buffer during 20 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Cell Biology 2024Quote: ... all input solutions were supplemented with 1 μg/mL of PE-conjugated rat anti-mouse TNFα antibody (MP6-XT22, BioLegend, UK).
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bioRxiv - Cell Biology 2024Quote: ... The cytokine capture bead for the infectious disease workflow was generated by conjugating an anti-mouse TNFα antibody (#6B8, BioLegend, UK) to 6.7 μm carboxyl polystyrene beads according to Spherotech guidelines.
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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bioRxiv - Immunology 2023Quote: ... and cellular surface markers were stained with the following fluorophore-conjugated anti-mouse antibodies: Alexa Fluor 488 CD3 (BioLegend, Cat# 100210), Alexa Fluor 488 CD19 (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated with 1:1000 chicken anti-GFP antibody (Aves GFP-1020) and 1:1000 mouse anti-tyrosine hydroxylase antibody (BioLegend 818001), with boosters over 5 days for a final concentration of 1:200 ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media was run in duplicate using an IL-1α ELISA (ELISA MAX Deluxe Set Mouse IL-1α, BioLegend Catalog # 433404).
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CD16/CD32 blocking antibody (Ultra-LEAF Purified Anti-mouse CD16/32 Antibody, Biolegend, San Diego, CA, USA), to prevent phagocytosis ...
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bioRxiv - Evolutionary Biology 2024Quote: Dissociated cells derived from forebrain and connective tissue were stained with APC-anti-mouse CD45 antibody (Biolegend, San Diego, CA; 103112), PE-Cy7-anti rat CD45 (Biolegend ...