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Citations for BioLegend :
401 - 450 of 1039 citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... anti-CTLA-4 (APC, clone BNI3 – BioLegend), anti-CD137 (APC-Cy-7 ...
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bioRxiv - Genomics 2024Quote: ... and SSEA-4 (PE anti-human, Biolegend). Pluripotency was also examined using PluriTest-RNAseq47.
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bioRxiv - Neuroscience 2023Quote: ... 4 ug/mL apolipoprotein E3 (BioLegend, USA) was mixed with fresh 10% FBS and applied to the cells for 1hr prior to shear and or fixing.
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bioRxiv - Cancer Biology 2022Quote: ... Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (Biolegend) per manufacturer’s instructions for HLA-A*02:01 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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bioRxiv - Developmental Biology 2021Quote: ... ENR media was supplemented with 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... were treated with 10 Gy (as described above) and either PMX205 or vehicle and either IL-10 blocking antibody (LEAF Purified clone JES5-16E3, Biolegend #505012) (50 μg 24 hours before irradiation ...
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bioRxiv - Neuroscience 2022Quote: ... Primary immunoreaction solution consisted of blocking buffer that contained 1:1000 rabbit anti-melanopsin (ATS Bio AB N38) and 1:250 mouse anti-SMI32 (Biolegend 801701). After incubation in the primary immunoreaction solution ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Genomics 2021Quote: Both iMGs and oMGs were resuspended in staining buffer and were blocked with Fc receptor blocking antibody (Human TruStain FcX, BioLegend 422302) for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with PBS supplemented with 1mM EDTA and resuspend in FcR blocking buffer using Human TruStain FcX (Biolegend, 422302) and Mouse TruStain FcX PLUS (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... biotinylated NP peptide loaded SLA monomers were freshly assembled into tetramer with streptavidin BV421 or BV650 (both from Biolegend, UK). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 minutes at 37°C and tetramers added to the cells on ice for another 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured in stimulation media without peptide stimulation or with phorbol 12-myristate 13-acetate (PMA) and ionomycin (BioLegend), respectively ...
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bioRxiv - Immunology 2021Quote: ... Undifferentiated donor 2395 iPSC were stained with anti-SSEA-4-APC and anti-Oct-4-PE (BioLegend, San Diego, CA) using the Fixation/Permeabilization Solution kit from BD Biosciences (San Jose ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411 ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-mouse IL-4 (Cat: 504127 Biolegend), APC-Cy7 anti-mouse CD3 (Cat ...
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bioRxiv - Immunology 2021Quote: IL-4 or IL-13 (Biolegend or PeproTech) was added into macrophage cultures at various concentrations to induce the M2/M2a phenotype in the presence or absence of a soluble CD47 extracellular domain (mCD47.ex or hCD47.ex) ...
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bioRxiv - Cancer Biology 2021Quote: ... PE antihuman CD262 (DR5) (Biolegend, Clone DJR2-4), PE anti-human TRAILR3 (DcR1 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD-4 APC (1:200, rat, Biolegend, 100411), FoxP3-PE (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... 4’,6-diamidino-2-phenylindole (1:10,000; Biolegend) was used.
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bioRxiv - Immunology 2020Quote: ... and anti-4-1BB–BV421 (clone 4B41; BioLegend).
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bioRxiv - Cell Biology 2021Quote: ELISA assay of different cytokines IL-4 (BioLegend), IL-5 (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... BV605-conjugated anti-human CD4 (OKT-4, BioLegend) and PE-Cy7-conjugated anti-human CD8 (SK1 ...
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bioRxiv - Bioengineering 2022Quote: ... or 20 ng/mL IL-4 (BioLegend 574302) for 48 h prior to use in tumouroid assays or prior to analysis of protein expression by immunofluorescence microscopy or flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-4 BV421 (Biolegend, clone MP4-25D2), and anti-IL-21 eFluor660 (Thermofisher ...
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bioRxiv - Immunology 2020Quote: ... and 10 μg/ml anti–IL-4 (Biolegend) One week after initial activation resting NR1 cells were co-incubated with untreated or IFNγ-treated macrophages of different genotypes ...
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bioRxiv - Immunology 2020Quote: ... APC Rat anti-mouse CD11a (M17/4, BioLegend), PE Armenian Hamster anti-mouse CD103 (2E7 ...
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bioRxiv - Immunology 2022Quote: ... and anti-IL-4-PE (clone 11B11, BioLegend), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... PE anti-human DR5 (clone DJR2-4; Biolegend), PE anti-human Fas (clone DX2 ...
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bioRxiv - Immunology 2022Quote: ... and anti-RT1D (BioLegend, #110211, 14-4-4S). Cells were subsequently fixed using IC fixation buffer (eBiosciences™ ...
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bioRxiv - Immunology 2022Quote: ... IL-4 (APC; 1:50; clone 11B11; BioLegend); IL-13 (PE ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of anti-HA antibody (BioLegend, 901513) was added to the remaining cleared lysate and incubated for 4 hours at 4 °C on an end-over-end rotator ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IL-4 (10 ug/mL, BioLegend), and anti- mouse IFN-γ (10 µg/mL ...