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Citations for BioLegend :
401 - 450 of 690 citations for 7 Oxa 12 thia 3 aza spiro 5.6 dodecane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used (mouseCD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and cells analyzed on the LSRFortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... 50µL of blood was stained for flow cytometry using conjugated antibodies (mouse CD45-FITC from BD bioscience and 7-AAD, humanCD45-PE/Cy7, humanCD19-PE, humanCD3-APC and humanCD14-APC/Cy7 from Biolegend) and analyzed on LSRFortessa flow cytometer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Microbiology 2021Quote: ... Cells were then filtered using a 40 μm cell strainer and stained with the Live/Dead dye 7 Amino-actinomycin D (7AAD, Biolegend) and antibodies against CD45.2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-V5 (BLD-680605, clone 7/4) and rabbit polyclonal anti-V5 (BLD-903801) were obtained from BioLegend. Mouse monoclonal anti-ICP36 (anti-UL44 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Genomics 2021Quote: ... Resultant cell pellets were resuspended in 50ul of antibody cocktail (all antibodies from Biolegend, Supplementary File 12) for 30 minutes before further washing and fixation in 2% paraformaldehyde ...
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bioRxiv - Microbiology 2022Quote: ... 96-well plates were coated with primary capture antibody against IL-12 (Biolegend Cat#511802, clone C18.2) and IL-1β (Biolegend Cat#503502 ...
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bioRxiv - Cell Biology 2019Quote: ... 3 and 6 were stained with Calcein-AM (BIOLEGEND, 425201) and PI (SIGMA ...
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bioRxiv - Immunology 2022Quote: ... 3 μL PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532), 0.0052 μg biotinylated Ovalbumin (SinoBiological ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD223 (LAG-3) antibody (clone 11C3C65, BioLegend), BV510 anti-human CD314 (NKG2D ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-Rpb3 (Neoclone W0012, 3 μL; BioLegend 665004, 1.5 μL), anti-Flag (Sigma-Aldrich F3165 ...
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used: anti-LAG-3 (11C3C65, BioLegend), anti-PD-1 (EH12.1 ...
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bioRxiv - Immunology 2020Quote: ... FITC anti-human CD223 (LAG-3) antibody (clone 11C3C65, BioLegend), BV510 anti-human CD314 (NKG2D ...
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bioRxiv - Immunology 2020Quote: ... CD127 AF488 (clone A019D5, BioLegend, cat# 351314, dilution 3:100); CD25 PE-Cy7 (clone BC96 ...
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bioRxiv - Immunology 2021Quote: ... washed for 3 times with Washing Buffer (BioLegend Cat#421601) and blocked with Dilution Buffer (0.5M Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with PE-Galectin-3 (Biolegend cat# 126706) or matched isotype control for 15 mins and analyzed on BD Accuri C6 cytometer (BD Biosciences ...
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bioRxiv - Genomics 2023Quote: ... TotalSeq™-B0253 anti-human Hashtag 3 Antibody (394635, Biolegend), TotalSeq™-B0254 anti-human Hashtag 4 Antibody (394637 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-Tubulin beta 3 (TUBB3) (Biolegend #Poly18020, 1:500), rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse TotalSeq-A Hashtag antibody (1-3; BioLegend). The stained T cell samples were then incubated with Sytox Green Dead Cell Stain (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... injection of 3 µg anti-CD45.2-PE (clone 104, Biolegend) antibody 3 min prior to sacrifice ...
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bioRxiv - Immunology 2024Quote: ... and anti-mouse α-CD28 (3 μg/ml, Biolegend, #102116) in RPMI medium supplemented with 10% FBS with or without dFB-CM ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... from BD Sciences (San Jose, CA); Alexa Fluor® 647-conjugated anti-rat Thy1 (OX-7, #202508; RRID:AB_492884) from BioLegend (San Diego, CA). Ghost Dye™ Violet 450 was from Tonbo Biosciences (#13-0863-T100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (1 × 106) were stimulated for 7 days at 37°C with anti-mouse CD3 (BioLegend; 1 μg/mL) and CD28 (BioLegend ...
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bioRxiv - Cell Biology 2019Quote: ... blocked with 1 mg/mL human IgG (Privigen, Behring CSL) and stained with mAb 7–239 α-Siglec-1-PE or matched isotype-PE control (Biolegend) at 4°C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... H1.2F3, BD, #553236; PE, H1.2F3, eBioscience, #12-0691-83; APC-Cy7, H1.2F3, BioLegend, #104526; BV785, H1.2F3, BioLegend, #104543), CD73 (APC Fire ...
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bioRxiv - Bioengineering 2022Quote: ... The hybridoma sample was stained with a 12 μg/mL solution of TotalSeqC conjugated anti IgG1 antibody (Biolegend) and incubated for 45 minutes on Ice ...
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Immunology 2023Quote: ... cells were treated with 81 nM phorbol 12-myristate 13-acetate (PMA) plus 1.34 µM Ionomycin (BioLegend, 423301) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Cytokines in the cell culture supernatants were quantified using Human Th Cytokine Panel (12-plex) (Cat. 741027, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... were stimulated with 500x Cell Activation Cocktail containing 40.5 μM phorbol-12-myristate 13-acetate (PMA) and 669.3 μM ionomycin (Biolegend) in the presence of 5 μg/mL BFA (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with cell activation cocktail (including phorbol 12-myristate 13-acetate and Ionomycin) (BioLegend) in the presence of Golgi Stop (monensin ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Neuroscience 2021Quote: ... Rat antibody against Notch 3 intracellular domain was obtained from Biolegend. Blots were probed with a mouse anti-actin (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Color development was stopped with 3 M H2SO4 stop solution (Biolegend). Purified mouse IgA (Southern Biotech ...
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bioRxiv - Cell Biology 2021Quote: Platelet subpopulations (3 × 106) stained with CD42b-BV421 (1:300; Biolegend) in (i ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 3% BSA/PBS cell staining buffer (BioLegend) and incubated at 37°C for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2019Quote: ... anti-Claudin 3 and anti-MHCII (M5/114.15.2, BioLegend, 1:150) were used in the TEC analysis ...
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bioRxiv - Immunology 2020Quote: ... and 1:50 of anti-TIM-3 BV785 (F38-2E2, BioLegend). Tetramer staining was performed in the dark at room temperature for 40 minutes ...