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Citations for BioLegend :
401 - 450 of 8591 citations for 5 1 3 Dioxolan 2 yl 2 3 fluorobenzoyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Rat antibody against Notch 3 intracellular domain was obtained from Biolegend. Blots were probed with a mouse anti-actin (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Color development was stopped with 3 M H2SO4 stop solution (Biolegend). Purified mouse IgA (Southern Biotech ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 3% BSA/PBS cell staining buffer (BioLegend) and incubated at 37°C for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Immunology 2020Quote: ... CD40 (APC anti-mouse CD40 Antibody, Clone 3/23 Biolegend 124611), or PD-L1 (APC anti-mouse CD274 (B7-H1 ...
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bioRxiv - Immunology 2020Quote: ... CD25 PE-Cy7 (clone BC96, BioLegend, cat# 302611, dilution 3:100); KLRG1 APC-Cy7 (clone 2FI/KLRG1 ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-Tubulin ß 3 antibody (Clone Tuj1, BioLegend, MMS-435P) respectively.
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with Cell Staining Buffer (BioLegend, Cat. No. 420201) and stained with APC-Annexin V (1:20 ...
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bioRxiv - Immunology 2022Quote: ... in the presence of 3 μg/ml anti-CD40 antibody (Biolegend) w/wo 50 ng/mL IL-21 ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 µM CHIR99021 (Axon) and 10 ng/ml mouse LIF (BioLegend)) ...
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bioRxiv - Immunology 2024Quote: ... and TIM-3(F38-2E2)-APC-Fire 750 (BioLegend (San Diego)) ...
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bioRxiv - Immunology 2024Quote: ... and TotalSeq™-A0303 anti-mouse Hashtag 3 Antibody (Biolegend, 155805).
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bioRxiv - Genomics 2024Quote: ... at a 1:300 dilution with DAPI 2 μl in 1 ml of 10 μg/ml (BioLegend) and kept on light rotation for 2 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 488 anti-CD54 antibody (2 μg/mL, ICAM-1, YN1/1.7.4, BioLegend), and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2021Quote: ... and Dectin-2 expression were examined by using rat anti-mouse Dectin-1 (Biolegend) followed by Alexa-594-conjugated goat anti-rat (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Immunology 2022Quote: ... media was changed to complete EGM-2 with 1 ng/mL TNFα (Biolegend 575206) and shear stress of 1 dyn/cm2 was incorporated using a microfluidic pump (Ibidi 10902 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... with the addition of either DCs (1 cell:3 Tresp) with 0.5 μg/ml purified anti-CD3 (145-2C11, Biolegend), or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Immunology 2023Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.
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bioRxiv - Immunology 2024Quote: ... Secreted IL-2 in the supernatant was measured using Human or Mouse IL-2 ELISA Kit (BioLegend). All incubations were performed at 37 °C / 5% CO2.
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bioRxiv - Immunology 2024Quote: ... the amount of IL-2 in the medium was quantified using Human IL-2 ELISA kit (Biolegend) or CD69 expression on Jurkat cells was measured by FACS ...
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bioRxiv - Immunology 2024Quote: ... while CD137 APC (2:100) and OX40 PE-Cy7 (2:100; Biolegend; clone Ber-ACT35; Cat# 350012) were used for CD4+ T cells.
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bioRxiv - Immunology 2023Quote: ... The cells were resuspended in 1 ml R10 and 1-2 million cells were plated in 1ml R10 with 1 μl Brefeldin A (BioLegend) for 4 hours ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromium Next GEM Single Cell 3’ Reagent v3.0 Kit (Biolegend, 10X Genomics). Libraries were sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... For intracellular antigens the following anti-mouse Galectin 3 (M3/38 BioLegend) was used.).
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bioRxiv - Immunology 2020Quote: ... and 3 μl of anti-human TCRab-AF488 antibody (clone IP26, Biolegend). After 2 washes in staining buffer (PBS containing 0.5% FBS and 2mM EDTA (pH 8.0) ...
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bioRxiv - Immunology 2020Quote: ... and 3 μl of anti-human TCRab-AF488 antibody (clone IP26, Biolegend), as described in the flow cytometry section above ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed every three days and supplemented with rmIL-3 (BioLegend), and BME (Gibco) ...
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bioRxiv - Pathology 2022Quote: ... Cells were then washed 3 times with cell staining buffer (#420201, BioLegend) followed by resuspension of the cells in DAPI containing FACS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL human recombinant IL-3 (BioLegend, San Diego, CA, USA) and antibiotics ...
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bioRxiv - Bioengineering 2024Quote: ... or (donors 3-4) anti-CD19 AF488 (BioLegend catalog #302219, clone HIB19), anti-CD3 PerCP/Cy5.5 (TONBO catalog #65-0037-T100 ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Staining was performed by washing with 0.1% Triton-X 100 in PBS for 3 x 15 minutes then staining for CD3-PE (1:100, Biolegend, 100205) overnight at room temp ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... anti-IgD-APC-Cy7 (Biolegend, IA6-2), anti-CD38-PE-Cy7 (Biolegend ...