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Citations for BioLegend :
4351 - 4400 of 4555 citations for Recombinant Mouse Csrp1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated with 1:1000 chicken anti-GFP antibody (Aves GFP-1020) and 1:1000 mouse anti-tyrosine hydroxylase antibody (BioLegend 818001), with boosters over 5 days for a final concentration of 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... The samples were washed with Cell Staining Buffer and stained with anti-mouse TCR β chain-Alexa Fluor® 647 (Biolegend) at a concentration of 0.02μg/μl ...
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bioRxiv - Microbiology 2023Quote: Lung single-cell suspensions were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody (Cat: 101320; BioLegend, San Diego, CA) in eBioscience™ Flow Cytometry Staining Buffer (Cat ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were generated by passing through 70 μm filters and sequentially stained with APC anti-mouse CD45 (Biolegend #103111) and propidium iodide (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: The quantification of cytokines was performed with the LegendPlex mouse Th cytokine panel according to the protocol provided by the manufacturer (Biolegend, USA). The amounts of cytokine per cell were calculated as follows ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Neurod1 (1/500, mouse monoclonal, hybridoma) (Kani et al., 2010), anti-paired box 2 [Pax2] (1/700, rabbit polyclonal) (BioLegend, 901001), anti-Foxp1b ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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bioRxiv - Developmental Biology 2023Quote: ... Then cells were again washed and followed by an incubation with anti-mouse IgG coupled to PE-Cy7 (Biolegend ref 406613) at a 1/200 dilution in BD wash buffer during 20 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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bioRxiv - Immunology 2023Quote: ... and cellular surface markers were stained with the following fluorophore-conjugated anti-mouse antibodies: Alexa Fluor 488 CD3 (BioLegend, Cat# 100210), Alexa Fluor 488 CD19 (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: Secreted cytokine levels into the collected IKKβCA- and Control-CMs were measured using 9-Plex LEGENDPlex mouse inflammation panel (BioLegend, Cat #740446) according to manufacturer’s protocol ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Maxpar Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) for FC blocking (10 min ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2023Quote: ... and labelled with anti-mouse TNF-ɑ (clone: Mab11, FITC), IFN-γ (clone: 4S.B3, BV-650) (BioLegend, San Diego, CA, USA), and GrzB (clone ...
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bioRxiv - Immunology 2023Quote: ... CD3+ CAR-T cells were then purified from lymphocytes using MojoSort™ Mouse CD3 T Cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media was run in duplicate using an IL-1α ELISA (ELISA MAX Deluxe Set Mouse IL-1α, BioLegend Catalog # 433404).
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CD16/CD32 blocking antibody (Ultra-LEAF Purified Anti-mouse CD16/32 Antibody, Biolegend, San Diego, CA, USA), to prevent phagocytosis ...
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bioRxiv - Evolutionary Biology 2024Quote: Dissociated cells derived from forebrain and connective tissue were stained with APC-anti-mouse CD45 antibody (Biolegend, San Diego, CA; 103112), PE-Cy7-anti rat CD45 (Biolegend ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Immunology 2024Quote: Production of IFNγ and IL-10 from T cell culture supernatant was measured using Legend Max ELISA Kit Mouse IFNγ (BioLegend, 430807) and Mouse IL-10 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend) in alignment with the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2020Quote: ... The following FACS antibodies were used at 1:200: mouse anti-human CD45 APC-Cy7 (BioLegend; Cat#304014; used at 1:200), mouse anti-human anti-CD11b-APC-Cy7 (BD ...
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bioRxiv - Cell Biology 2020Quote: ... Around 900 μl of plasma from each mouse was used to stain for T cell (1:150, APC-CD3e/clone 145-2C11, Biolegend Cat. 100312), B cell (1:300 ...
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bioRxiv - Immunology 2021Quote: The IFN-β levels in cell supernatants and mouse sera were measured with ELISA kits purchased from BioLegend (San Diego, CA, USA).
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 10 min at RT in 1.0 μg anti-mouse CD16/32 antibody (BioLegend, San Diego, CA, USA) containing 500 μl FACS buffer for Fc receptor blocking ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with primary antibodies diluted in 1 % BSA/0.3 % Triton X- 100/DPBS (mouse anti-HA (BioLegend, 901501; 1:1000), mouse anti-myc (NEB/Cell Signaling ...