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Citations for BioLegend :
4351 - 4400 of 10000+ citations for Rabbit Anti Sheep IgG H+L Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Cells were then stained with a panel of 9 further DNA-oligonucleotide conjugated antibodies (0.33 μg/ml anti-CD71: BioLegend 113802 conjugated with ADT9 [CGAAGAAGGAGT], 1 μg/ml anti-CD41: BioLegend 133919 conjugated with ADT3 [TGTCCGGCAATA] ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Immunology 2022Quote: ... surface markers (PE/Cyanine7-labeled anti-mouse CD3, Cat# 100220, BioLegend; APC-labeled anti-mouse CD4, Cat# 100412, BioLegend; PE-labeled anti-mouse CD8 ...
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bioRxiv - Immunology 2022Quote: ... anti-granzyme B PE (1:50; clone QA16A02) and anti-TNFα BV711 (1:50; clone MP6-XT22) from BioLegend in permeabilization buffer at 4°C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD49a APC (1:50; clone HMα1) and anti-CD11b BV570 (1:100; clone M1/70) from BioLegend. Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with FACS buffer and stained with anti-CD3 PerCp-Cy5.5 and anti-CD279 BV421 (Biolegend) and Live/dead fixable near IR APC-Cy7 in FACS buffer for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-APC (90, eBioscience), anti-IgM-PE/Cy7 (II/41, eBioscience) and anti-IgD-PerCP/Cy5.5 (11-26c.2a, Biolegend). The working concentration of the antibodies is 2.5μg/ml.
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Aβ (anti-β-amyloid 17-24, clone 4G8, #800712, 1:500, Biolegend, San Diego, CA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and stained with PE anti-IL-6 and PE-Cy7 anti-IL-10 (JES3-9D7) Abs (Biolegend).
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bioRxiv - Immunology 2021Quote: ... PBMCs were stimulated with 2 μg/mL of indicated COVID-19 antigen peptide pools (RBD or Spike or NP) in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37 □ overnight ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Microbiology 2020Quote: ... For surface stains, anti-mouse TCRβ (eBioscience, San Diego, CA) and anti-mouse CD4 antibodies (Biolegend, San Diego, CA) were used ...
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bioRxiv - Immunology 2020Quote: ... and incubated with biotin conjugated anti-CD8 (53-6.7) and anti-CD19 (6D5) at a dilution of 1:200 (both from Biolegend) and 50μL/mL normal rat serum for 10 min at RT ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in blocking solution (2% BSA in PBS) and immunolabeled with anti-CD24 PerCP/Cy5.5 and anti-CD44 FITC primary antibodies (BioLegend, 1:50 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell suspensions were stained with anti-SM-C/2.6 antibody (Fukada et al., 2004) as well as anti-CD45-PE (diluted 1/500, Biolegend), anti-CD31-PE (diluted 1/500 ...
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bioRxiv - Immunology 2024Quote: ... Intracellular cytokine staining of APC-labeled anti-IFNγ (clone: XMG1.2; eBioscience) and FITC-labeled anti-TNFα (clone: MP6-XT22; BioLegend) were performed using the BD Cytofix/Cytoperm ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were subsequently stained with CD47-PerCPCy5.5 and CD26-PE antibodies (Anti-human CD47 PerCP/Cy5.5 conjugate, Biolegend, Cat#323110, 1:200; Anti-human CD26 PE conjugate, Biolegend, Cat#302705 ...
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bioRxiv - Immunology 2022Quote: Human cells were surface stained for 25 minutes at 4 °C with antibodies to with anti-CD3 (Clone UCHT1, eBioscience) and anti-CD56 (Clone 5.1H11, Biolegend).
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bioRxiv - Immunology 2023Quote: Cell surface FcεRI and c-kit stained by PE-labeled anti-mouse FcεRIα Ab (MAR-1, eBioscience) and FITC-labeled anti-mouse CD117 Ab (2B2, BioLegend) were detected by a MACS Quant (Miltenyi Biotech) ...
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bioRxiv - Molecular Biology 2023Quote: ... and then incubated for 1 hr with either anti-RpoS or anti-RpoD antibodies (BioLegend, San Diego, CA, USA) in PBS-T containing 0.3% nonfat milk or ECL PrimeTM blocking agent ...
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bioRxiv - Immunology 2023Quote: ... Immunostaining was performed using the following primary antibodies: rat anti-mouse CD8α (clone 53-6.7, eBioscience) and Armenian hamster anti-mouse CD11c (clone N418, BioLegend). CD8 primary staining signal was further amplified using an anti-rat IgG secondary antibody conjugated with Alexa Fluor (AF ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Biochemistry 2023Quote: ... monoclonal mouse anti-HA 11 epitope (catalogue number: 901502) and mouse anti-Tubulinβ3 (catalogue number: 657402) were from BioLegend. Polyclonal rabbit anti-IKKα/β (H-470) ...
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... and the endothelial cells were separated using Dynabeads coupled to anti-CD31 antibody and anti-ICAM2 antibody (Biolegend, USA) according to the manufacturer’s instructions (Thermo fisher scientific ...
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bioRxiv - Cell Biology 2020Quote: ... containing 1% BSA and incubated with rabbit monoclonal CTR1 antibody (ab129067) or an isotype control antibody (Biolegend, clone Poly29108) for 1 hour on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... Pacific Blue anti-mouse CD31 (rat, clone 390), anti-mouse CD45 (rat, clone 30-F11), anti-mouse Ter119 (rat, clone TER-119) were from BioLegend; APC anti-mouse CD326 or EpCAM (rat ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Immunology 2019Quote: ... mice were injected intraperitoneally with 100μg anti-TNFα (Ultra-LEAF™ Purified anti-mouse TNFα Antibody, clone MP6-XT22 Biolegend) or 200μg intraperitoneally (ip ...
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bioRxiv - Immunology 2019Quote: ... the membrane expression of BTLA and HVEM were assessed using respectively an APC conjugated anti-BTLA and a PE conjugated anti-HVEM (Biolegend). Cells were analyzed on a LSRII (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Undifferentiated donor 2395 iPSC were stained with anti-SSEA-4-APC and anti-Oct-4-PE (BioLegend, San Diego, CA) using the Fixation/Permeabilization Solution kit from BD Biosciences (San Jose ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
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bioRxiv - Bioengineering 2020Quote: ... cells were first incubated in a cocktail of PE anti-mouse CD169 and PE anti-mouse F4/80 antibodies (BioLegend). After washing ...
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bioRxiv - Bioengineering 2021Quote: ... Naïve T cell marker staining was performed with APC/Cy7 mouse anti-human CD45RA (#304128) and BV510 mouse anti-human CD45RO (#304232) antibodies from Biolegend.
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bioRxiv - Immunology 2021Quote: Staining for tumor-associated neutrophils was performed in processed tumor/biopsies samples with anti-CD45-PercP and anti-CD15-PE (Biolegend) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1/250), anti-mCD71 mAb (RI7217, dilution 1/250) and anti-mTNFα mAb (MP6-XT722, dilution 1/250) from Biolegend; anti-mCD69 mAb (H1.2F3 ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used to identify MKs: 1:100 anti-mouse CD41-FITC+ and anti-mouse CD42d-APC+ (BioLegend); and MKPs ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibodies (anti-GFAP, DAKO, z-0334, 1:1000; SMI-99 [anti-myelin basic protein] Biolegend; 808401 ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504], anti-human CD158b [KIR2DL2/L3, NKAT2]-FITC [BioLegend, Cat#312604] ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...