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Citations for BioLegend :
4351 - 4400 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend) and quenched by 0.5 M sulfuric acid (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... The single cell suspensions of retinas were stained with Zombie AquaTM Fixable Viability Kit (BioLegend) for 20 min and then washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... The MojoSort Mouse Naïve CD4+ T cell Isolation Kit (#480040, BioLegend, San Diego, CA, USA) was used for the isolation of naïve CD4+ T cells as previously described (15) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were stained for viability using Zombie NIR or Zombie Aqua Fixable Viability kits (BioLegend) for 15 minutes in the dark at 4°C ...
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bioRxiv - Immunology 2024Quote: ... CD4+ cells were purified with the MojoSortTM mouse CD4 T cell isolation kit (Biolegend, 480006) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... the first to block endogenous biotin and avidin with an Avidin/Biotin Blocking Kit (BioLegend).
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bioRxiv - Cancer Biology 2023Quote: ... Viability of cells were assessed with the Zombie GreenTM Fixable Viability Kit (BioLegend, Cat. #423111) APCs and patient-derived glioma cell lines were stained with HLA-A2 monoclonal antibody (Clone BB7.2 ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated by negative selection using the Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058). Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were then sorted by negative selection using Mojosort Mouse Neutrophil Isolation Kit (Biolegend 480058) and stained with 100 nM dichlorofluorescein diacetate (DCFDA ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated using MojoSort Mouse CD8 T Cell Isolation Kit (480035, BioLegend). CD8+ T cells were cultured and expanded in a similar fashion to γδ T cell populations ...
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bioRxiv - Immunology 2023Quote: ... Live/dead cell discrimination was performed using Zombie Aqua™ Fixable Viability Kit (BioLegend, 423102). Intracellular staining was performed using a FOXP3 Fixation Kit (eBioscience) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed and stained with a viability dye (Zombie Aqua Fixable viability kit, BioLegend) and fixed with 100µl of pre-warmed Cytofix Fixation Buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... using the MojoSort™ CD8+ T-cell isolation kit and magnets (Biolegend, #480008 and #480019). Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco ...
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bioRxiv - Systems Biology 2023Quote: ... the first to block endogenous biotin and avidin with an Avidin/Biotin Blocking Kit (Biolegend). Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining for transcription factor (Foxp3) was performed on using True-Nuclear Factor kit (Biolegend) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with a viability dye (Zombie NIR™ Fixable Viability Kit, #423106 BioLegend or LIVE/DEAD™ Fixable Aqua ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... Dead cells were excluded by using DAPI or the Zombie NIR fixable viability kit (BioLegend). Intracellular cytokines were assessed following stimulation with PMA (25 ng/ml ...
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bioRxiv - Immunology 2023Quote: Cells were stained with Carboxyfluorescein diacetate succinimidyl ester (CFSE) Cell Division Tracking Kit (BioLegend, 423801), and then cultured in complete RPMI1640 with IL-2 for 3 days ...
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bioRxiv - Cancer Biology 2023Quote: ... single-cell suspensions were first stained using the Zombie NIR fixable viability kit (Biolegend, 423105). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with Zombie NIR™ or Zombie Aqua™ Fixable Viability Kit (BioLegend) for 15 minutes in RT ...
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bioRxiv - Immunology 2023Quote: ... Live-dead cell discrimination was performed using Zombie Aqua™ Fixable Viability Kit (423102, Biolegend) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Live-dead cell discrimination was performed using Zombie Aqua™ Fixable Viability Kit (423102, Biolegend) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: Naive CD8 T cells were isolated from SLO using Mojosort isolation kits (Biolegend, Dedham MA). Mice received 5×103 congenically-marked donor cells by intravenous (i.v. ...
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bioRxiv - Immunology 2023Quote: ... macrophages were challenged with CFSE (5-[and 6]-Carboxyfluorescein diacetate succinimidyl ester Kit, BioLegend,423801)-labeled apoptotic cells at a 4:1 (AC/Macrophage ...
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bioRxiv - Cancer Biology 2024Quote: ... and Zombie Aqua™ Fixable Viability Kit 500 tests (cat# 423102) were purchased from Biolegend. Cell lysis buffer (cat# 9803S ...
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the femurs of mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Immunology 2024Quote: The PE-Annexin V/7-amino-actinomycin D (7-AAD) apoptosis detection kit (BioLegend, UK) was used to detect apoptosis according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... and then labeled with Carboxyfluorescein Succinimidyl Ester (CFSE) using CFSE Cell Division Tracker Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice in PBS and stained with Zombie UV Fixable Viability Kit (Biolegend) according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... granulocyte and macrophage (1:1000, PECy7-Gr-1/clone RB6-8C5 and PECy7-CD11b /clone M1/70, Biolegend Cat.108416 and Cat.101216), then analyzed by flow cytometry (BD MoFlo XDP).
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bioRxiv - Immunology 2021Quote: ... cells were stained in PBS at 4°C for 30 minutes with the fixable viability dye eFluor 780 (1:1000, 65-0865-14 eBioscience) and a combination of the following antibodies (1:200, all from BioLegend unless stated otherwise): anti-mouse CD45 BV650 (30-F11 ...
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bioRxiv - Microbiology 2019Quote: ... Latently infected CD14+ monocytes were fixed with 1% paraformaldehyde and stained using anti-HLA-DR Pacific blue conjugate (Biolegend, Clone L243, 1:50) and anti-HLA-A,B,C ...
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bioRxiv - Genomics 2020Quote: ... Cells were blocked with 1% BSA/PBS for 30 minutes and then incubated with antibodies for HA.11 (BioLegend MMS-101P, 1:2000) and BIP (Abcam ab21685 ...
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bioRxiv - Immunology 2019Quote: ... After isolation cells were treated with Fc receptor blocking solution and stained with anti-ICAM-1-PE (1 μg/ml, HA58; Biolegend, San Diego, USA), anti-VCAM-1-BV711 (0.5 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 100 µl containing 1 × 106 cells in PBS were incubated with yellow Zombie viability dye (1:1000 dilution, Cat. No 423104, Biolegend, San Diego, CA) for 15 minutes at room temperature followed by washing in FACS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Cell Biology 2020Quote: ... after blocking cells were incubated with 1:250 dilution of rabbit anti-TbKH pAb and 1:500 dilution of mouse anti-HA mAb (BioLegend, Cat# MMS-101R). As a negative control ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Immunology 2022Quote: ... CD11bhi, F4/80hi) were isolated by FACS using antibodies against CD11b (eBioscience, #11-0112, 1:1600) and F4/80 (Biolegend, #123114, 1:200), and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... or biotinylated anti-repebody antibody (1 μg/mL, AbClon, Seoul, Korea) and HRP-conjugated streptavidin (1:1000 dilution, BioLegend, San Diego, CA, USA). For trastuzumab (Herceptin) ...
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bioRxiv - Immunology 2021Quote: ... Bacteria were then resuspended in 0.2µm-filtered solutions of appropriate secondary reagents (Alexa 647-anti-human IgG, Jackson Immunoresearch 1:200, Brilliant Violet 421-anti-Rabbit IgG, Biolegend 1:200). This was incubated for 10-60 min before cells were washed as above and resuspended for acquisition on a BD LSRII or Beckman Coulter Cytoflex S ...
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bioRxiv - Immunology 2021Quote: ... followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). After enrichment for S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated at 4°C overnight with rabbit sera against MmuPV1 L1 (a kind gift form Chris Buck, NIH; 1:2500) and chicken anti-keratin 14 (BioLegend 905301; 1:500) diluted in 5% goat serum in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were suspended in 300 μL staining buffer and incubated on ice for 30min with FVD780(eBioscience,1:1000) and then with CD45(Biolegend,202205, 1:300) for 30min ...
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bioRxiv - Immunology 2021Quote: ... Cell sorts were performed with MojoSort Mouse Pan B Isolation Kit II (BioLegend, San Diego, CA) for CD19+ cells and with Miltenyi’s magnetic-activated cell sorting (MACS ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... For the exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) was adjoined and incubated for 30 min dRT ...
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bioRxiv - Immunology 2021Quote: ... and for exclusion of dead cells Zombie Aqua fixable viability kit (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in FACS buffer and stained using a Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 minutes at 4°C ...