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Citations for BioLegend :
4301 - 4350 of 5248 citations for Mouse MEP1B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Mouse cytokines and chemokines were quantitated in graft and spleen tissue using the LEGENDplex Mouse Inflammation and custom plex panel (Biolegend) according to manufacturer’s instruction 22 ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used at the indicated concentrations (IHC-immunohistochemistry; ICC-immunocytochemistry; IB-immunoblot): purified anti-HA mouse (Biolegend Cat# 901501 ...
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by a 10 min incubation with anti-mouse Fc block and a 30 minute cell surface staining with anti-mouse CD4-APC/Cy7 and CD8-PE/Cy7 (Both from Biolegend). CFSE labeled CD8+ CTLs were subsequently sorted on a BD FACS Aria III (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin) with mouse anti-human monoclonal antibody CD62P-AlexaFluor647 (Clone AK4, Cat.No. 304918, BioLegend, USA). and activation associated conformational change in integrin αIIbβ3 was detected with a mouse anti-human monoclonal antibody PAC1-FITC (Clone PAC-1 ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... The homogenates were then resuspended in PBS buffer and stained with anti-mouse CD45.1 antibody conjugated with Brilliant Violet 421 (clone A20; BioLegend, 103134), CD11b monoclonal antibody conjugated with APC (M1/70 ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were incubated with APC (allophycocyanin) or PE (phycoerythrin)-conjugated anti-mouse Ly-6A/E (Sca-1) antibody (#122508, BioLegend), APC or PE-conjugated anti-mouse CD45 antibody (#103106 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... Measurements were compared to the isotopic control (APC-conjugated anti-mouse IgG1, Biolegend clone MOPC-21 #BLE400122, 1/100, Ozyme) to determine background and positivity thresholds ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the tissues were washed multiple times in PBS at room temperature for one day and put into a staining solution containing Alexa Fluor 488 anti-mouse CD41 antibody (clone MWReg30, BioLegend) at a 1:100 dilution and Alexa Fluor 555 donkey anti-goat antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... PE anti-human TRAILR4 (DcR2) (R&DSystems, Clone 104918) or PE Mouse IgG1 κ Isotype Control (Biolegend, Clone MOPC-21) for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: C26 tumor-bearing mice were injected intraperitoneally with 200 μg of GR1-depleting antibody (Ultra-LEAF anti-mouse-Ly6G/Ly6C Ab, BioLegend). Control mice were injected intraperitoneally with 200 μg of isotype control (Ultra-LEAF rat IgG2b ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702; CD11b, BioLegend, 101202 ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the in vivo samples to obtain absolute counts of events acquired on the cytometer as well as using human and mouse Fc blocking antibodies (Biolegend) to reduce non-specific antibody staining ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). Finally ...
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bioRxiv - Bioengineering 2022Quote: ... and Phycoerythrin-conjugated Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody (Fisher) for ACE2-Bite and APC anti-human IgG Fc Antibody clone HP6017 (Biolegend) for ACE2-Fc were added to the wells and incubated for another hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)), CCR3 (a Th2 marker (28)), or CCR6 (a Th17 marker (29, 30)) antibodies (BioLegend). For the MHC ...
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bioRxiv - Immunology 2022Quote: ... the processed fecal samples are incubated with autologous diluted serum and stained as previously described with the following fluorophore-conjugated antibodies: goat anti-mouse PE-IgA and PE-Cy7-IgG1 (clone RMG1-1; Biolegend). Flow cytometry analysis on stained bacteria populations was performed on the LSRFortessa and data analysis was performed on FlowJo ver ...
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bioRxiv - Immunology 2022Quote: ... Cell samples at each timepoint were prepared as described and co-stained with PE-anti-CD19 and allophycocyanin-conjugated mouse anti-human CD23 (APC-anti-CD23, BioLegend, clone EBVCS-5 ...
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bioRxiv - Physiology 2022Quote: ... the cell pellet was resuspended in 100 μl of Alexa Fluor 647-conjugated anti-human/mouse SSEA1 antibody (catalog no. MC-480; BioLegend) diluted 1:100 in PBS + 2.5% FBS and incubated at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Alexa Fluor® 488-conjugated or PE/Cy7-conjugated rat anti-mouse CD326 (EpCAM) antibody (1:200, BioLegend, clone G8.8), Alexa Fluor® 647-conjugated rat anti-mouse CD104 (integrin β4 ...
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bioRxiv - Microbiology 2022Quote: ... and then stained for 30 minutes at 4℃ with the following antibody panel each diluted in PBS: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend) or FITC anti-mouse CD45.2 antibody (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... Surface staining was performed with anti-mouse Fc receptor antibody (clone 2.4G2, UCSF Hybridoma Core) or Human TruStain FcX (BioLegend #422302) in PBS with 2% FCS for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in culture supernatant or mouse plasma were measured with the LegendPLEX Inflammation Panel (13-plex) or the Mouse Th1 Panel (5-plex) per the manufacturer protocols (BioLegend). Quantification was done using LegendPLEX software v8.0 for Windows ...
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bioRxiv - Microbiology 2021Quote: ... cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3, Biolegend), a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Immunology 2020Quote: ... Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32) Antibody (both BioLegend, London, UK) were used to stain according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Immunology 2022Quote: ... IL-17A, IL-17F, IL-21, IL-22) and mouse Pro-inflammatory Chemokine Panel (CCL20, CXCL1, CCL2, CCL3, CCL4) (Biolegend) using a FACSVerse flow cytometer (BD Bioscience) ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM EDTA and 1% BSA) and incubated with the following primary antibodies: anti-mouse CD45 (Brilliant Violet 570, 30-F11, 1:200, Biolegend), anti-mouse Fce-R1 alpha (PE-Cyanine7 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were pelleted by centrifugation and washed in FACS buffer before incubation with goat anti-mouse Alexa Fluor 647 (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood was surface stained with APC/Cy7 conjugated anti-mouse CD45 antibody (clone 30-F11, Biolegend, San Diego, CA), APC conjugated anti-mouse CD3 antibody (clone 145-2C11 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend). Cell surface markers were stained using APC-coupled AH1 tetramers and fluorochrome-conjugated antibodies against CD8a (FITC) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor sections were then incubated with fluorochrome-conjugated antibody (1:100, Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40 ...
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bioRxiv - Immunology 2020Quote: In some cases we injected retro-orbitally with 10 μg of Alexa Fluor 647 anti-mouse CD31 (BioLegend, no. 110724) and 10 μg of Alexa Fluor 647 anti-mouse CD144 (BioLegend ...
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bioRxiv - Genomics 2020Quote: ... Cytokine concentrations in super-natants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
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bioRxiv - Immunology 2019Quote: ... Naive CD4+ and CD8+ T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (Biolegend): TCR γ/δ (clone GL3) ...
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bioRxiv - Bioengineering 2020Quote: ... Unbound cells were collected and cultured for 24 hours in the presence of 1 μg/ml anti-mouse CD3ε (145-2C11, BioLegend) and 0.5 μg/ml CD28 (37.51 ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Pathology 2020Quote: Bead-based multiplex assays were employed to quantify cytokines (LEGENDplex™ mouse Inflammation Panel, BioLegend Inc., San Diego, CA, USA) in plasma samples obtained from mice at pre and postinfection stages ...