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Citations for BioLegend :
4301 - 4350 of 4492 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Immunology 2020Quote: ... splenocytes from OT-II/Rag1/CD45.1 mice were cultured in a-CD28 coated plates (Biolegend, Cat. No. 102115, 1ug/ml) with complete RPMI (10%FBS ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Cell Biology 2022Quote: ... lung cells were brought to a single-cell suspension via collagenase incubation and then stained for flow cytometry at a concentration of 5×106 cells/mL with Cd31 (Biolegend) and 3μM ALOD4-47.
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bioRxiv - Microbiology 2022Quote: ... the supernatant was discarded and the pellet was resuspended in 10 ml of cold 1X Red Blood Cell Lysis buffer (Biolegend). Samples were vortexed and incubated in the dark for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and either activated them with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... we obtained splenocytes from WT and TCF-7 cKO mice and stimulated T cells with 2.5ug/ml anti-CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Immunology 2022Quote: ... Anti-Mac-1 was reconstituted by mixing anti-CD11b (clone ICRF44) and CD18 (clone TS1/18) mAbs at a final concentration of 10 µg/mL for each (BioLegend). Anti-CD3 mAbs (clone UCHT1 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed once with ice-cold FACS buffer and incubated with 1 μg/mL PE anti-human IgG Fc (Biolegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend) and anti-IFN-γ (R&D Systems) ...
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bioRxiv - Biochemistry 2022Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/ml erythropoietin) for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID ...
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bioRxiv - Immunology 2021Quote: IFNAR1 on WT and Mt3-/- BMDMϕ was neutralized using 10 μg/ml monoclonal anti-IFNAR1 antibody (BioLegend; Clone: MAR1-5A3) 1h prior and 24h after iLPS (10 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed three times in ice cold blocking buffer and re-suspended in blocking buffer containing 0.1 μg/ml DAPI (Biolegend, USA).
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bioRxiv - Immunology 2022Quote: ... The BM cells per animal were spined down and then resuspended in 1 mL Red Blood Cell Lysis (RBCL) buffer (Biolegend) at room temperature for 1 min to lyse the red blood cells ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Immunology 2019Quote: Enriched CD8+ were co-cultured with THP1 cells loaded with fixed E.coli at 30 BpC in the presence or absence of 20ug/mL LEAF™ anti-MR1 Antibody (Biolegend). Supernatants were collected at 72 hours ...
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bioRxiv - Immunology 2020Quote: ... single cell suspension of lung cells were washed and resuspended at 20 ×106 cells/mL in PBS + Zombie NIR Live/Dead stain (Biolegend) at 2μL/mL and incubated for 20 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Unbound cells were collected and cultured for 24 hours in the presence of 1 μg/ml anti-mouse CD3ε (145-2C11, BioLegend) and 0.5 μg/ml CD28 (37.51 ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor cell lines were plated in triplicate on 96-well plates and treated for 72 hours with 10 ng/mL TNF (Biolegend) or 0.1% BSA vehicle control ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... cultured with WKYMVm for 24 h were collected in 1.5 ml Ep tubes after washing twice and were incubated with Alexa Fluor-488 (AF488, BioLegend, USA)-conjugated anti-mouse CD206 and activated protein C (APC ...
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bioRxiv - Cancer Biology 2020Quote: ... Single tumor cells were resuspended to 5 × 107 cell/mL and stained with CD298-APC (10 µL/106 cells)(BioLegend) and Sytox Green (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... T-25 or T-75 flasks were coated with 5 µg/mL anti-CD3ε antibodies (clone 145-2C11; 100340, BioLegend) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Heparinized blood diluted 1:1 in PBS overlaid onto Lymphopure density gradient medium 1.077 g/mL (BioLegend, San Diego, USA) in Leucosep tubes (Greiner Bio-One ...
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bioRxiv - Immunology 2021Quote: ... Induced GC B-cells were then replated onto a fresh CD40LB feeder line and cultured with mouse IL-21 (10 ng/mL; BioLegend) or mouse IL-4 for another 3 to 4 days.
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bioRxiv - Microbiology 2021Quote: ... for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend), and intracellular staining of anti-human Arginase1 FITC (Ref ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were removed from Myc-eGFP mice and mashed and cell suspension activated with anti-CD3 (1 µg/mL, clone 145-2C11, Biolegend) and anti-CD28 (3 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
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bioRxiv - Immunology 2021Quote: ... Primary bone marrow-derived murine macrophages (BMDMs) were pretreated with PBS or murine IL-27 (50 ng/ml from BioLegend) for 24 hours and then infected with S ...
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bioRxiv - Cell Biology 2021Quote: ... at a final concentration of 2.5μg/ml and we run in parallel samples with IgG isotype control (BioLegend, Cat#400526). All antibodies were centrifuged for 10 min at 14,000 x g before use ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2022Quote: Cells isolated from murine livers were stained with 30 μg/mL Hoechst 33342 (Miltenyi) and Zombie NIR viability dye (diluted 1:250 in PBS; Biolegend) before FACS sorting (Sony SH800 ...
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bioRxiv - Immunology 2022Quote: ... M1 cells were generated by addition of 20ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF, Cat. No. 572904, BioLegend) and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Physiology 2022Quote: ... CD11b+-SVF were incubated with were with 0.2 mg/mL of anti-mouse PE-F4/80 (BioLegend, #12-4801-82) with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Microbiology 2024Quote: ... and where indicated containing 25 ng/mL human granulocyte-macrophage colony-stimulating factor (GM-CSF) ((# 572903, Biolegend, San Diego, CA) at 37 °C in a humidified chamber with 5% CO2 in 96-well ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...