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Citations for BioLegend :
4251 - 4300 of 4555 citations for Recombinant Mouse Sfrp1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... samples were incubated with 20 μL of staining buffer containing 0.5 μL of TruStain FcX anti–mouse CD16/32 blocking agent (BioLegend 101319) for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and 2.5 mM HEPES into 6-well plates pre-coated for more than 1 h with 5.4 µg of Ultra-LEAFTM purified anti-mouse CD3e antibody (clone 145-2C11, Biolegend, # 100340) and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated from the spleen and lymph nodes of FVB/N mice using the MojoSort Mouse CD8 T cell isolation kit (BioLegend) as per the manufacturer’s protocol for negative selection strategy ...
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bioRxiv - Bioengineering 2024Quote: Cytokine measurements were carried out on plasma (2x diluted) or cell culture supernatant (undiluted) with a LegendPlex 13-plex Mouse Inflammation Panel (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... cells were treated with 10 µg/ml of Ultra-LEAFTM purified anti-mouse IFNγ antibody (clone R4-6A2, Biolegend, #505709), 10 µg/ml of Ultra-LEAFTM purified anti-mouse TNF antibody (clone MP6XT22 ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... the NPCs were blocked by 1% anti-mouse CD16/CD32 for 10 min and then stained with AF647 anti-F4/80 (1:200, BioLegend) for 20 min ...
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bioRxiv - Microbiology 2024Quote: Cytokine concentrations in supernatants from cultured BMDMΦ or sera of animals were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (18 ...
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines present in the sera of uninfected vs MCMV-infected mice and in the BAL of uninfected vs IAV-infected mice were analyzed using the LEGENDplex Mouse Anti- Virus Response Panel (BioLegend). Samples and standards were plated in technical duplicates and the assay was executed according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulations were performed by first coating a 96-well plate with 5 μg/mL anti-mouse CD3 (Biolegend) in 50 μL/well PBS overnight the day before tissue harvest ...
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bioRxiv - Immunology 2024Quote: ... 0.1−0.5×106 cells were incubated for 15 min at 4 °C with anti-mouse Fc-block CD16/32 antibody (clone 93, 1:100) and True-Stain Monocyte Blocker (1:100) (both from Biolegend), in 100 µl FACS staining buffer (1.5% FBS and 0.5% BSA in PBS) ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Immunology 2024Quote: ... We stained the cells with Fixable Viability Dye eFluor 780 (eBioscience, San Diego, CA) and eFluor 506-conjugated anti-mouse CD45 (BioLegend), PE-conjugated anti-mouse CD3e (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... The following anti-mouse antibodies were used for flow cytometry: anti-CD45 BV711 (Biolegend Clone, clone 30-F11, lot B366812); anti-CD3 PE (Biolegend Clone 17A2 ...
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bioRxiv - Cell Biology 2021Quote: ... Then 1×106 cells were washed again with 1x Hanks buffer and stained with 5µl of Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody (Biolegend#125608) in100µl BD Pharmingen™ Stain Buffer (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked using PBS/1% BSA/0.5% Tween-20 and incubated with monoclonal mouse FITC anti BrdU (Biolegend, clone 3D4) at 1:50 dilution for 20 minutes ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were stained with PE-coupled anti-Acetylated Lysine Antibody or PE-coupled Mouse IgG2b κ Isotype control Antibody (isotype control) (both Biolegend) for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Immunology 2019Quote: ... total CD4+ T cells were first isolated from Foxp3Thy1.1 reporter mice(Liston et al., 2008) by using mouse CD4 T cell isolation kit (Biolegend, catalog 48006), and stained with anti-Thy1.1-PE antibody (eBioscience ...
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bioRxiv - Immunology 2019Quote: ... The cells were incubated with the following antibodies (1:100) for 30 minutes at room temperature: Alexa Fluor 488 anti-mouse CD25 (Biolegend, 102017), PE/Cy7 anti-mouse/human CD44 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and stained in 100 μl for 30 minutes on ice using an anti-mouse IgG-Brilliant Violet421 secondary antibody at 1:90 (BioLegend, 405317). Cells were again washed twice ...
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bioRxiv - Immunology 2021Quote: ... 0.1% BSA) before secondary detection with 1:100 dilutions of APC- conjugated mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Microbiology 2021Quote: Aliquots of cells collected before and after MACS separation were analysed by flow as described above after staining with BV510 anti-mouse CD71 (Biolegend, #103145), PE/Dazzle-594 anti-mouse-CD45 (Biolegend ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Bioengineering 2020Quote: ... anti-Pax7 (mouse monoclonal, DHSB, diluted 1:4) and anti-Neurofilament H phospho (rabbit polyclonal, BioLegend # PRB-573C, diluted 1:400). As secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was blocked in 5% milk followed by probing with either α-HA Mouse Monoclonal (Catalogue #HA.11; Biolegend, SanDiego, CA) or α-Rsp5 Rabbit Polyclonal [84] antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked using DMEM/FBS media for 1 hour at RT followed by incubation with 1:1000 dilution mouse anti-HA (Biolegend, 901514) and rabbit anti-PCNA (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... Annexin V staining was performed on PDX AML-388 cells isolated from the mouse BM 72h after TAM treatment using PE/Cy7 Annexin V (BioLegend, 640949) according to the manufacturer’s instruction and analyzed by flow cytometry (LSRII ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... and the secondary Alexa Fluor donkey 488-conjugated anti-mouse IgG pAb (Thermo Fischer Scientific, USA) and the Alexa Fluor 667-conjugated rat anti-mouse CD31 mAb (clone MEC133, Biolegend, USA).
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bioRxiv - Immunology 2022Quote: CD4+ T cells (1 × 106) were stimulated for 7 days at 37°C with anti-mouse CD3 (BioLegend; 1 μg/mL) and CD28 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Global inflammatory cytokine analysis of supernatants of adipose tissue explant cultures and serum were performed using LEGENDPlex™ Mouse Inflammation Panel (740446, Biolegend). TNFα and IL-10 levels were measured by TNFα Mouse Uncoated ELISA Kit (88-7324-86 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary immunoreaction solution consisted of blocking buffer that contained 1:1000 rabbit anti-melanopsin (ATS Bio AB N38) and 1:250 mouse anti-SMI32 (Biolegend 801701). After incubation in the primary immunoreaction solution ...
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bioRxiv - Bioengineering 2022Quote: ... OT-I T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (BioLegend; San Diego, CA): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2022Quote: ... the plate was washed five times with PBST and incubated with anti-mouse secondary antibody with minimal cross reactivity with human IgG (Biolegend, 405306). The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-lung suspension was then repelleted and resuspended in SB + 1:50 TruStain FcX™ antibody (anti-mouse CD16/32, BioLegend) for a 10-minute blocking period at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells from each strain were then labeled with TotalSeq™-A0301 anti-mouse Hashtag Antibody against CD45 and MHC-I (BioLegend, Clone M1/42 and 30-F11 ...
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bioRxiv - Immunology 2022Quote: ... for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11), CD11b (BioLegend clone M1/70 ...