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Citations for BioLegend :
4201 - 4250 of 4330 citations for Beta NGF Mouse 110a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Immunology 2022Quote: ... The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet™ fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11 ...
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bioRxiv - Microbiology 2022Quote: ... the remaining volume was subjected to immunoprecipitation on protein A/G magnetic beads coated with 5 µg of mouse anti-HA IgG (Biolegend, clone 16B12) as described in the Magna RIP technical manual ...
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated by negative selection with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... FITC (or APC)-conjugated anti-CD177 mAb (clone MEM-166, mouse IgG1) and respective isotype controls were from BioLegend (San Diego, CA). FITC-conjugated goat anti-human Ig(H+L ...
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF) 488 mouse anti-human BAX antibody (0.5 μg/mL, Biolegend, Cat. No. 633603) or AF488 Mouse IgG1 κ isotype control (5 μg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Selection for transgene-positive cells was confirmed by flow cytometry for mouse Lyt2 expression as a reporter gene (Biolegend, San Diego, CA). 3T3 cells were grown in DMEM with 10% FBS and 1% P/S ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Immunology 2023Quote: HLA-B heavy chain and β2-microglobulin (β2m) were probed using HC10 (mouse hybridoma procured from University of Michigan Hybridoma Core) and purified anti-β2m (Biolegend; catalog # 316302) antibodies.
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4degC with the following fluorochrome conjugated primary anti-mouse antibodies for 45 minutes (all Biolegend, San Diego, CA): anti-CD45 PE-CF594 (clone 30-F11) ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Cancer Biology 2023Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: CD4+ cells were isolated from pooled splenocytes and lymph node cells with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 100 µl of 105/ml aAPC-BLMs containing all three ligands were stained with 5 µl of anti-mouse IgG PE (BioLegend, Cat#406607) and anti-human HLA-DR PE (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cancer Biology 2023Quote: The following antibodies were used for flow cytometry: Brilliant Violet 510™ anti-mouse CD45 Antibody (BioLegend, catalog #103137, clone 30-F11), PE/Dazzle™ 594 anti-mouse CD3ε Antibody (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... were rehydrated in distilled H2O then stained with Nissl or immunohistochemical labelling was performed as above with anti-mouse Ly6G primary antibody (BioLegend, London, UK) and goat anti-rabbit secondary antibody.
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: Cytokine production was quantified from cell supernatants or serum using the following ELISA kits: ELISA MAX Deluxe Set Mouse IL-10 (BioLegend, Cat# 431414), ELISA MAX Deluxe Set Human IL-10 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from in vitro treated CAR-T cells using Mojosort™ Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). After washing cells with PBS twice ...
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bioRxiv - Cancer Biology 2023Quote: ... M- CSF and GM-CSF differentiated BM cells were cultured with complete medium +10ng/ml recombinant mouse M-CSF (Biolegend, cat.no. 576408) or +20ng/ml recombinant GM-CSF (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... and stained overnight with Alexa 488-conjugated Alexa Fluor® 488 anti-tubulin β3 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Neuroscience 2024Quote: ... cell preparation and cell surface marker staining was carried out as previously described.20 The following antibodies were used for staining: mouse anti-CD11b (Biolegend, Cat# 101257), mouse anti-CD19 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... followed by flow cytometric analysis of the uptake of fluorescent OVA and the presentation of the OVA257-264 (SIINFEKL) by mouse MHC-I (H2Kb) using a PE-conjugated anti-H2Kb-SIINFEKL antibody (Biolegend; clone 25-D1.16). To further confirm the role of S100A4 in promoting antigen cross-presentation ...
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bioRxiv - Neuroscience 2019Quote: ... Red blood cells were osmotically lysed and CD4+ T-cells were isolated using a MojoSort Mouse CD4+ T Cell Isolation Kit (Biolegend, San Diego, CA). Purified CD4s were labeled with Cell Proliferation Dye eFluor450 (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... 1×106 P14 or pmel splenocytes were co-stained with Db-GP33 liposomes (10 μg/ml) and anti-mouse CD8-PE (Biolegend, Clone: 53-6.7) for 30 min on ice ...
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bioRxiv - Bioengineering 2021Quote: P14 CD8+ T cells were isolated as described above and incubated with DiO-labeled Db-GP33 liposomes and anti-mouse CD8 (Biolegend, Clone: 53-6.7) at 4°C or 37°C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Axons were identified using a staining against the phosphorylated neurofilaments (SMI31) stained with the PAB mouse-anti-SMI31 (Biolegend, cat. 801601, 1:250) and the SAB GAM-STAR580 (Abberior ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostaining was performed with biotinylated monoclonal mouse antitubulin (clone 236-10501, Thermo, 1 μg/ml) then Brilliant Violet 421-conjugated streptavidin (Biolegend, 0.1 μg/ml), Alexa Fluor 488-conjugated mouse anti-perforin (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... after blocking cells were incubated with 1:250 dilution of rabbit anti-TbKH pAb and 1:500 dilution of mouse anti-HA mAb (BioLegend, Cat# MMS-101R). As a negative control ...
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bioRxiv - Genomics 2022Quote: ... CD45+ tumor-infiltrating leukocytes were stained with 2.5 μg/ml of Totalseq anti-mouse Hashtag-A antibodies (A0301-A0314, BioLegend, San Diego, CA, USA) at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Cell Biology 2022Quote: ... single cell suspensions of WT intestinal epithelium were stained with CytoID as detailed above and incubated with anti-mouse Hashtag antibodies (BioLegend Cat #155839, 155841,155843,155845). CytoID high and low cells were then sorted and processed separately for sequencing library generation ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were resolved by SDS-PAGE and detected by Western blotting using mouse monoclonal antibodies against σ70/σA (clone name 2G10, Biolegend, cat. No. 663208), against the β subunit of RNAP (clone name 8RB13 ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Cell Biology 2021Quote: ... Nerves were harvested at 7 days post-injury to assess vascularization and axon regeneration via antibody reactivity to mouse anti-TUJ1 for microtubules in axons (Biolegend Catalog No. 801202) and rat anti-PECAM-1 for endothelial cells within vessels (Biosciences ...