Recombinant Human APCS Protein citations for BioLegend

Autologous human immunocompetent white adipose tissue-on-chip

Julia Rogal, et al., bioRxiv - Bioengineering 2021

Quote: ... Cytokines were determined by fluorescent bead-based multiplex sandwich immunoassays (LEGENDplex™ Human Angiogenesis Panel 1, 740697 and LEGENDplex™ Human Adipokine Panel, 740196; BioLegend, Inc., San Diego, CA) read by flow cytometry (Guava easyCyte 8HT ...

Rat Leukocyte Population Dynamics Predicts a Window for Intervention in Aging

Hagai Yanai, et al., bioRxiv - Evolutionary Biology 2022

Quote: ... PE-Cy7 conjugated anti-rat CD11b/c (clone OX-42, Cat#201818), APC-Cy7 conjugated anti-rat CD4 (clone W3/25, Cat#201518) from Biolegend (San Diego, USA), and AF647-conjugated anti-rat RT1B (clone OX-6 ...

Immune disease variants modulate gene expression in regulatory CD4+ T cells and inform drug targets

Lara Bossini-Castillo, et al., bioRxiv - Genetics 2019

Quote: ... The cells were stained with the following antibody cocktail: anti-CD4-APC (30 µl/ml final volume, clone OKT4, Cat. no. 317416, BioLegend, San Diego, U.S.), anti-CD127-FITC and (30 µl/ml ...

Dual role of protease activated receptor 4 in acute kidney injury: contributing to renal injury and inflammation, while maintaining the renal filtration barrier upon acute renal ischemia reperfusion injury

Marcel Jansen, et al., bioRxiv - Immunology 2019

Quote: ... Platelet count and platelet activation were determined by flow cytometry (FACS Calibur, Becton Dickinson, Franklin Lakes, NJ) using hamster anti-CD61-APC monoclonal antibody (BioLegend, San Diego, CA) and anti-CD62p-FITC (BD Biosciences ...

Whole genome sequencing of a sporadic primary immunodeficiency cohort

James E. D. Thaventhiran, et al., bioRxiv - Immunology 2020

Quote: ... CD27 – PE-Cy7 (eBioscience, San Diego, CA,USA, CD62L – APC-eF780 (eBioscience, San Diego, CA,USA, CXCR3 – FITC (Biolegend, San Diego, CA, USA), CXCR5 – AF488 (Biolegend ...

Immunomodulatory Contribution of Mast Cells to the Regenerative Biomaterial Microenvironment

Raymond M. Wang, et al., bioRxiv - Bioengineering 2021

Quote: ... 22 and 28 before differentiation of mast cells was assessed by flow cytometry with antibody markers for CD117 APC (2B8, Biolegend, San Diego, CA) and FcεRI PE (MAR-1 ...

Outer Membrane Vesicles from the gut microbiome contribute to tumor immunity by eliciting cross-reactive T cells

M. Tomasi, et al., bioRxiv - Microbiology 2021

Quote: ... incubated 15 minutes on ice and then stained at RT in the dark for 20 minutes with the following mixture of fluorescent-labeled antibodies: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...

The parathyroid hormone-dependent activation of osteoblasts enhances hematopoietic stem cell migration and reduces their engraftment abilities

Yasmine Even, et al., bioRxiv - Cell Biology 2021

Quote: ... anti-CD201 antibodies conjugated to PE and anti-CD48 antibodies conjugated to APC or PE-Cy5 were purchased from Biolegend (San Diego, CA). To stain dead cells ...

Inhibition of mitochondrial dynamics preferentially targets pancreatic cancer cells with enhanced tumorigenic and invasive potential

Sarah Courtois, et al., bioRxiv - Cancer Biology 2021

Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...

scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells

Agostina Bianchi, et al., bioRxiv - Genomics 2022

Quote: ... Bone marrow mononuclear cells were stained for 30 minutes on ice with the following antibodies: anti-CD19(APC/Cy7) (clone HIB19; Biolegend; Cat. num. 302217) in a 1:20 dilution ...

CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses

Sallieu Jalloh, et al., bioRxiv - Immunology 2022

Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...

Exploring heteroaromatic rings as a replacement for the labile amide of antiplasmodial pantothenamides

Jinming Guan, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...

Single-cell sequencing of plasma cells from COVID-19 patients reveals highly expanded clonal lineages produce specific and neutralizing antibodies to SARS-CoV-2

Roy A. Ehling, et al., bioRxiv - Immunology 2021

Quote: ... followed by a single wash and a secondary staining with anti-mFc IgG1: PE and/or APC [clone RMG1-1] at 1:100 (Biolegend, Cat: 406608/406610). After enrichment for S1 ...

SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease

Raphael A. Reyes, et al., bioRxiv - Immunology 2021

Quote: ... Spike and RBD tetramers were synthesized by incubating biotinylated protein with fluorophore-conjugated streptavidin overnight at 4°C (streptavidin-PE, Tonbo, cat. no. 50-4317-U100, streptavidin-APC, Tonbo, cat. no. 20-4317-U100, streptavidin-BV421, BioLegend, cat. no. 405226) at a molar ratio of 5:1 ...

Hypoxia-inducible factor-2 (HIF2) regulates alveolar regeneration after repetitive injury

A. Scott McCall, et al., bioRxiv - Cell Biology 2023

Quote: ... Cells were suspended at 3×106 cell/ml and incubated for 30 min at 4C with 1:100 dilutions of the following antibodies: [APC dump channel: Ter-119 (Biolegend cat no. 116212), CD45 (Biolegend cat no ...

Flexible and scalable control of T cell memory by a reversible epigenetic switch

Kathleen Abadie, et al., bioRxiv - Immunology 2023

Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...

Development of a Robust Consensus Modeling Approach for Identifying Cellular and Media Metabolites Predictive of Mesenchymal Stromal Cell Potency

Alexandria Van Grouw, et al., bioRxiv - Bioengineering 2023

Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...

Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to vaccination

Duncan M. Morgan, et al., bioRxiv - Immunology 2023

Quote: Biotinylated pneumococcal serotype 3 polysaccharide with a 5% biotin load was coupled with high concentration streptavidin-phycoerythrin (SA-PE) and streptavidin-allophycocyanin (SA-APC, both from BioLegend, San Diego, CA) in separate reactions ...

Increased Susceptibility of WHIM Mice to Papillomavirus-induced Disease is Dependent upon Immune Cell Dysfunction

Wei Wang, et al., bioRxiv - Microbiology 2023

Quote: ... A combination of selected antibodies (anti-mouse) was used depending on the purpose of each study: CD45 APC-Cy7 (Biolegend, clone 30-F11), CD8a FITC (Tonbo ebioscience ...

Versatile workflow for cell type resolved transcriptional and epigenetic profiles from cryopreserved human lung

M Llamazares Prada, et al., bioRxiv - Cell Biology 2020

Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...

Notch Signaling Commits Mesoderm to the Cardiac Lineage

Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...

Patient-derived triple negative breast cancer organoids provide robust model systems that recapitulate tumor intrinsic characteristics

Sonam Bhatia, et al., bioRxiv - Cancer Biology 2022

Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...

Longitudinal Assessment of SARS-CoV-2 Specific T Cell Cytokine-Producing Responses for 1 Year Reveals Persistence of Multi-Cytokine Proliferative Responses, with Greater Immunity Associated with Disease Severity

Jonah Lin, et al., bioRxiv - Immunology 2022

Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD4 (BV711 ...

Longitudinal Assessment of SARS-CoV-2 Specific T Cell Cytokine-Producing Responses for 1 Year Reveals Persistence of Multi-Cytokine Proliferative Responses, with Greater Immunity Associated with Disease Severity

Jonah Lin, et al., bioRxiv - Immunology 2022

Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD3 (APC-Cy7 ...

Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity