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Citations for BioLegend :
3951 - 4000 of 4293 citations for Mouse ADAMTS16 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... then Fc gamma receptors were blocked for 15 minutes with heat-inactivated human serum prepared in-house or anti-mouse CD16/32 (TruStain FcX, Biolegend) for human samples and mouse samples respectively ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were assessed for titers of different antibody isotypes using LegendPlex Mouse Immunoglobulin Isotyping Panel and IgE ELISA (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Mo were then isolated from the suspension of differentiated BMC using a MojoSort™ Mouse Monocyte Isolation Kit (BioLegend #480154). The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 expression on KPC cells was assessed with an allophycocyanin-conjugated anti-mouse PD-L1 Ab (clone 10F.9G2; BioLegend). Flow cytometry was performed using a Cytek Aurora (Cytek ...
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bioRxiv - Cancer Biology 2023Quote: ... or cells cultured in vitro were subjected for surface marker staining with the following reagents for 20 min at 4 °C: Brilliant Violet 650TM-conjugated anti-mouse CD3 Ab (clone 17A2; BioLegend), PerCP/Cyanine5.5-conjugated anti-mouse CD4 Ab (clone GK1.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated single-cell suspension and peripheral leukocytes were fluorescently stained with anti-mouse CD11b-FITC (1:200, BioLegend, 101205) and Ly6G-PE (1:80 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a premixed cocktail of the indicated tetramers at a final concentration of 10 nM for 1 hour at RT in the presence of anti-mouse CD16/32 antibody (clone 93, BioLegend). SLO preparations were magnetically enriched for tetramer-specific cells as previously described64 while lung samples were magnetically enriched for CD90.2 expression (Miltenyi) ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Physiology 2023Quote: ... Serum samples were prepared for Legendplex assays by diluting 1:50000 for the detection of immunoglobulin isotypes using the mouse immunoglobulin isotyping kit (BioLegend). The assay was performed in a V-bottom plate and data was acquired on a BD Fortessa X-30 cytometer (BD Biosciences) ...
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bioRxiv - Physiology 2023Quote: ... single cell suspension from mouse esophagi was incubated at 37°C for 30 minutes with Integrin β1 antibody (PE anti-mouse/rat CD29; 102207; Biolegend) at 1:200 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with FACS buffer and pre-incubated with 5 μg/mL TruStain FcX anti-mouse CD16/32 antibody (clone 93, BioLegend) in 100 μL FACS buffer for 5 minutes on ice before immunostaining ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used were Fluoroscein isothiocyanate (FITC)-conjugated anti-mouse CD4 and CD21/CD35 (CR2/CR1: Biolegend, San Diego, CA, USA) and Phycoerythrin (PE)-conjugated anti-mouse CD3 (17A2 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for western blot were: Mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), goat anti-Mouse DKK-3 (1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... The BALF was processed to isolate the cells upon RBC lysis and stained with PE anti-mouse Ly6G clone 1A8 (Biolegend) and FITC anti-mouse F4/80 clone BM8 (Biolegend ...
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bioRxiv - Physiology 2023Quote: ... the resulting single cell suspension was incubated at 37°C for 30 minutes with Integrin β1 antibody (APC anti-mouse/rat CD29; 102216; Biolegend) at 1:100 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... The culture supernatant was collected and the Th2 cytokines were measured using a LEGENDplex™ mouse Th cytokine assay (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained for sgRNA expression with an APC conjugated anti-mouse CD90.1/Thy1.1 antibody (no. 202526, BioLegend; 1:400) and Human TruStain FcX Fc receptor blocking solution (no ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from the spleen of OT- I mice using the MojosortTM Mouse CD8+ T cell isolation kit (Biolegend) and stained with 5µM CellTrace Violet (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF ...
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bioRxiv - Immunology 2024Quote: ... We stained the cells with Fixable Viability Dye eFluor 780 (eBioscience, San Diego, CA) and eFluor 506-conjugated anti-mouse CD45 (BioLegend), PE-conjugated anti-mouse CD3e (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the MDSC panel consisted of the following primary antibodies diluted in FACS buffer: anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin-streptomycin for 16 h before exposure to recombinant mouse IL-6 (rIL-6) at 50 ng/ml (575702, BioLegend) or vehicle (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated from the spleen and lymph nodes of FVB/N mice using the MojoSort Mouse CD8 T cell isolation kit (BioLegend) as per the manufacturer’s protocol for negative selection strategy ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: Cytokine measurements were carried out on plasma (2x diluted) or cell culture supernatant (undiluted) with a LegendPlex 13-plex Mouse Inflammation Panel (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and 2.5 mM HEPES into 6-well plates pre-coated for more than 1 h with 5.4 µg of Ultra-LEAFTM purified anti-mouse CD3e antibody (clone 145-2C11, Biolegend, # 100340) and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... cells were treated with 10 µg/ml of Ultra-LEAFTM purified anti-mouse IFNγ antibody (clone R4-6A2, Biolegend, #505709), 10 µg/ml of Ultra-LEAFTM purified anti-mouse TNF antibody (clone MP6XT22 ...
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bioRxiv - Immunology 2024Quote: Cytokines and chemokines present in the sera of uninfected vs MCMV-infected mice and in the BAL of uninfected vs IAV-infected mice were analyzed using the LEGENDplex Mouse Anti- Virus Response Panel (BioLegend). Samples and standards were plated in technical duplicates and the assay was executed according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Microbiology 2024Quote: Cytokine concentrations in supernatants from cultured BMDMΦ or sera of animals were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (18 ...
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bioRxiv - Microbiology 2024Quote: ... the NPCs were blocked by 1% anti-mouse CD16/CD32 for 10 min and then stained with AF647 anti-F4/80 (1:200, BioLegend) for 20 min ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulations were performed by first coating a 96-well plate with 5 μg/mL anti-mouse CD3 (Biolegend) in 50 μL/well PBS overnight the day before tissue harvest ...
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bioRxiv - Immunology 2024Quote: ... 0.1−0.5×106 cells were incubated for 15 min at 4 °C with anti-mouse Fc-block CD16/32 antibody (clone 93, 1:100) and True-Stain Monocyte Blocker (1:100) (both from Biolegend), in 100 µl FACS staining buffer (1.5% FBS and 0.5% BSA in PBS) ...
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bioRxiv - Microbiology 2024Quote: ... LAMP-1 was stained as described previously for LAMP-2 but using a rat-IgG anti-mouse LAMP-1 and anti-rat-IgG-AlexaFluor647 (Biolegend) [56] ...
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bioRxiv - Microbiology 2024Quote: ... samples were incubated with 20 μL of staining buffer containing 0.5 μL of TruStain FcX anti–mouse CD16/32 blocking agent (BioLegend 101319) for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Immunology 2024Quote: ... The following anti-mouse antibodies were used for flow cytometry: anti-CD45 BV711 (Biolegend Clone, clone 30-F11, lot B366812); anti-CD3 PE (Biolegend Clone 17A2 ...