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Citations for BioLegend :
351 - 400 of 3751 citations for Testosterone 100 Ug Ml In Methylene Chloride 3 4 13C2 99%; 17 18O 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed three times in FACS buffer and resuspended in FACS buffer containing 3 μg/ml DAPI (BioLegend). Flow cytometric analysis was done on a BD LSRFortessa analyzer and sorting was done on a BD FACSAria1 machine.
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bioRxiv - Immunology 2020Quote: ... These cells were stimulated with 10μg/ml of anti-CD3 (OKT-3) and anti-CD28 (28.2) antibodies (Ultra-LEAF™, Biolegend) in R10-50-15 for 48 hours ...
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bioRxiv - Immunology 2021Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2021Quote: ... and stained with 1 ug/test cell-hashing antibody (TotalSeq C0251, C0254, C0256, C0260, clones LNH-95, 2M2, BioLegend) for 30 minutes at 4C ...
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bioRxiv - Immunology 2023Quote: ... each sample was labelled with 0.1 ug of a cell hashing antibody (TotalSeq-B anti-human Hashtag antibodies 1 through 8, BioLegend). Cells were labelled for 30 minutes on ice in the dark ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Immunology 2023Quote: ... Th17 differentiation media consisted of complete media supplemented with 2 μg/mL anti-IL-4 (Cat# 504101; Biolegend), 2 μg/mL anti-IFNγ (Cat# BE0055 ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Pathology 2019Quote: ... The tissue had been previously stained with an amyloid-β antibody (4G8, recognizing residues 17-24, BioLegend, formerly Covance) that were first pretreated with formic acid to rid samples of endogenous protein ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Aβ (anti-β-amyloid 17-24, clone 4G8, #800712, 1:500, Biolegend, San Diego, CA, USA) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Molecular Biology 2019Quote: ... for 3 min and stained using mouse anti-human HER2 antibody conjugated to FITC (20 μg/ml, clone 24D2, BioLegend). Cells were incubated with anti-HER2 antibody for 20 min at 4°C in the dark and washed once with PBS supplemented with 1% FCS prior to the FACS analysis ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... CD40 (3/23, BioLegend), CD115 (CSF-1R ...
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bioRxiv - Cancer Biology 2022Quote: ... LAG-3 (BioLegend, 369212) or LFA-1 (BD ...
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bioRxiv - Immunology 2022Quote: ... CD40 (3/23) (Biolegend), CD11b (M1/70) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tim-3 (BV510, BioLegend), LAG-3 (BV605) ...
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bioRxiv - Bioengineering 2023Quote: ... Histone 3 (BioLegend #819411), YAP/TAZ (Santa Cruz #sc-101199) ...
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bioRxiv - Immunology 2023Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... TIM-3-BV711 (Biolegend), LAG-3-APC-eFluor 780 (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... caspase-3 (W20054B; Biolegend), cleaved caspase-3 (Asp175 ...
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bioRxiv - Immunology 2021Quote: ... 108 yeast were pelleted and resuspended in 1 mL PBE (PBS with 0.5% BSA and 0.5 mM EDTA) containing 1:100 anti-FLAG PE antibody (BioLegend). Yeast were stained at 4° for 75 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... we collected the beads and labeled them with 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6) for 45 min at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... eF450 (anti-GL7, eBioscience, 48-5902-82), APC (anti-CD86, eBioscience, 17-0862-82) and PE (anti-CXCR4, BioLegend, 146505). Also ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Immunology 2023Quote: Supernatants from stimulated T cells were harvested on day 2 and day 6 depending on peak of cytokine production and analyzed using the LEGENDplexTM human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...