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Citations for BioLegend :
351 - 400 of 1124 citations for Protein Labeling kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
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bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
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bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Plant Biology 2024Quote: ... The protein levels were detected by immunoblot analysis using the primary antibodies: anti-HA antibody (Biolegend, #901503, dilution 1:5000), anti-FLAG antibody (CST ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Bioengineering 2021Quote: ... by negative selection with MojoSort™ Mouse CD4+ T cell Isolation Kit and MojoSort™ Mouse CD8+ T cell Isolation Kit (Biolegend, USA).
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bioRxiv - Neuroscience 2022Quote: ... ELISA kit (CSB-E18033h, Generon) and monomeric α-Syn was analyzed using LEGEND MAX™ Human α-Synuclein ELISA Kit (SIG-38974, BioLegend). Then it was normalized by total protein per well using Pierce BCA Protein Assay Kit (23225 ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell, cat. no. 17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, cat. no. 480082) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... viable T cells were enriched using the MojoSort Mouse CD3 T Cell Isolation Kit and Mouse Dead Cell Removal Kit (BioLegend, San Diego, CA).
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was immunodepleted to remove soluble amyloid beta or mock immunodepleted using protein A agarose beads (Thermo, 20334) with 4G8 antibody (Biolegend, 800703) or mouse serum (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag) conjugated with Brilliant Violet 605 Streptavidin and TotalSeq-C0973 Streptavidin (BioLegend, 405275) and TotalSeq-C0974 Streptavidin(BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Neuroscience 2022Quote: Global protein concentration of the BV2 cells post treatment was assessed via flow cytometry using intracellular protein staining using the True-Nuclear Transcription Factor Buffer Set (Biolegend # 424401). Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Immunology 2023Quote: To determine galectin-3 or galectin-8 expression the following: intracellular protein and lysosomes were stained using anti-Lamp1-BV421 (clone H4A3, BioLegend, USA), anti-Galectin-3-AF488 (clone B2C10 ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b+CD45.2+CX3CR1+ microglia were sorted into a pre-cooled 5ml low-protein binding falcon tube (fisher scientific; Cat#: 13-864-407) containing 0.5ml cell staining buffer (BioLegend, Cat#: 420201). Walls of the tubes were washed and cells pelleted at 300g ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... stained with Zombie Aqua™ Fixable Viability Kit (BioLegend, 423101) and fixed with IC fixation buffer according to manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD117 (c-kit) (Biolegend, clone 104D2; 1:100); anti-human CD148 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Zombie UV Fixable Viability Kit (BioLegend, San Diego, CA, USA) was used to determine cell viability ...
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bioRxiv - Microbiology 2019Quote: ELISA kits for the following cytokines: IFNβ (LEGEND MAXTM, BioLegend) and TNFα (ELISAkit.com) ...
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bioRxiv - Immunology 2019Quote: ... Alexa Fluor 488 anti-mouse CD117 (c-Kit) (Biolegend, 105816), PE/Dazzle-594 anti-mouse Ly-6A/E (Sca-1 ...
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bioRxiv - Immunology 2019Quote: ... and anti-CD117/c-kit (2B8) were purchased from Biolegend. Anti-CD43 (S11) ...
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bioRxiv - Immunology 2021Quote: ... Serum IgE concentrations were measured using an ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... and TNF-⍰ ELISA kits were from Biolegend (San Diego, CA). Human IL-13 and IL-17A ELISA kits were from eBioscience (San Diego ...
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bioRxiv - Bioengineering 2021Quote: ... and Legend Max Mouse CXCL13 (BLC) ELISA kit (BioLegend, 441907) were used to quantify different serum cytokines ...
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bioRxiv - Bioengineering 2020Quote: ... fixed and permeabilized using a FoxP3 Fix/Perm Kit (BioLegend), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MojoSort mouse CD4 T cell isolation kit was from BioLegend. Liberase TL (Research Grade ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines were measured using human ELISA Max deluxe kits (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2022Quote: ... 1) AnnexinV/7-AAD staining (Kit from Biolegend Cat# 640922): At the end of the EMT experiment described above ...
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bioRxiv - Bioengineering 2022Quote: ... and assayed with Annexin V/7-AAD staining kit (BioLegend). The cells were stained with Annexin V diluted in 1x Annexin V buffer for 15 minutes and incubated with 2x 7-AAD for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... the FITC Annexin V Apoptosis Detection Kit with PI (Biolegend) was used ...
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bioRxiv - Immunology 2022Quote: ... MojoSort human CD14+ monocytes isolation kit was purchased from Biolegend. Cell culture media DMEM and RPMI-1640 were purchased from Hyclone.
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bioRxiv - Molecular Biology 2022Quote: ... and The LEGEND MAX Human IL-1β ELISA Kit (Biolegend) was used to assay for IL-1β concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... Zombie Green™ Fixable Viability Kit (1:1000, Biolegend 423111) or DAPI (1:10000 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stained with Zombie NIR Fixable Viability kit (Biolegend) (1:400 ...
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bioRxiv - Cancer Biology 2021Quote: ... HGF was quantified using a kit from RayBiotech and total TGFβ1 using a kit from Legend Max (BioLegend).
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bioRxiv - Microbiology 2022Quote: ... 10 μl of SA-PE provided with the kit (BioLegend) was added to each well ...