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Citations for BioLegend :
351 - 400 of 6151 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Fixation and permeabilization was performed with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (cat # 00-5523-00) before intracellular staining with anti-Foxp3-PE (MF-14, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Microbiology 2024Quote: ... and where indicated containing 25 ng/mL human granulocyte-macrophage colony-stimulating factor (GM-CSF) ((# 572903, Biolegend, San Diego, CA) at 37 °C in a humidified chamber with 5% CO2 in 96-well ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... M1 cells were generated by addition of 20ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF, Cat. No. 572904, BioLegend) and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF ...
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bioRxiv - Immunology 2023Quote: ... and additional step was performed using the Foxp3/Transcription Factor Staining Bugger Set (cat. no. 00-5523, eBioscienceTM) and FOXP3-BV421 or FOXP3-PE (Clone 150D, Biolegend) according to manufacture instructions ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF ...
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bioRxiv - Immunology 2024Quote: ... while M2 macrophages were induced with 20 ng/mL of macrophage–colony-stimulating factor (M-CSF, Cat. No. 574804, BioLegend). After 6 days ...
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Inhibition of CELA1 Improves Septation in the Mouse Hyperoxia Model of Impaired Alveolar DevelopmentbioRxiv - Developmental Biology 2024Quote: ... For intracellular staining, cells were washed and fixed (room temperature, 30 minutes) with the True-Nuclear Transcription Factor Buffer set fixative (BioLegend) and permeabilized (4°C ...
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bioRxiv - Microbiology 2020Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were collected by centrifugation (1500rpm; 5mins; 4°C) and washed twice with Annexin V binding buffer (Biolegend; 1ml per wash). The cell pellets were resuspended in of binding buffer (100μl ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Neuroscience 2021Quote: ... intracellular staining was performed on single cell suspensions according to instructions provided by True-Nuclear™ Transcription Factor Buffer Set (Biolegend). The antibodies used were anti-IL-1β PE (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: Cells obtained from embryos and stained with Epha1 antibodies as described above were then washed twice with PBS/10% DS and processed for staining using True-NuclearTM transcription factor Buffer Set (BioLegend #424401) according to the manufacturer’s instructions and following the protocol described in (Aires et al. ...
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bioRxiv - Neuroscience 2022Quote: Global protein concentration of the BV2 cells post treatment was assessed via flow cytometry using intracellular protein staining using the True-Nuclear Transcription Factor Buffer Set (Biolegend # 424401). Cells were incubated with primary antibodies for 30 minutes - 1:100 Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Genomics 2022Quote: ... supernatants were removed and cells fixed according to the manufacturer’s instructions utilizing the True-Nuclear™ Transcription Factor Buffer Set (Biolegend, 424401). Intracellular staining was performed according to manufacturer’s instructions before FACS analysis was carried out ...
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bioRxiv - Neuroscience 2024Quote: ... and preMG were resuspended in 3:1-medium supplemented with 50 ng/mL recombinant human MCSF (Macrophage Colony Stimulating Factor, BioLegend, #574804). Cells were counted using an automated cell counter (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: 30 µL peptide-exchanged monomer formed in the above steps was mixed with 3.3 µL PE streptavidin (BioLegend) on a new plate and incubated on ice in the dark for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Thrombin Receptor Activator Peptide-6 (TRAP-6; 30μM) or Adenosine Diphosphate (ADP; 30μM) along with CD42b-BV421 (1:60, Biolegend), PAC-1 FITC (1:12 ...
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bioRxiv - Immunology 2021Quote: Biotinylated DPB1*04:01-monomers loaded with TFEYVSQPFLMDLE peptide (S167-180) were tetramerised using PE-Streptavidin (Biolegend, 405204). 1.1 volume PE-conjugated streptavidin was added to 1 volume of DPB1*04:01-monomer (1 mg/mL) ...
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bioRxiv - Immunology 2024Quote: ... Cells from peripheral blood were isolated and washed with FACS buffer (PBS with 1% BSA) and single cell suspensions were blocked for FcγR binding (BioLegend clone 93; 1:50) and then stained with the following antibodies to validate B cell depletion ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with antibody cocktail for surface markers at 4 °C for 30 min and then fixed using True nuclear transcription factor buffer set (Biolegend, Cat # 424401) for 20 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular interferon γ (IFNγ) and tumor necrosis factor α (TNFα) were stained with anti-IFNγ-Alexa Fluor 488 (clone 4S.B3, Biolegend, Netherland) and anti-TNFα-BV421 antibodies (clone Mab11 ...
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bioRxiv - Immunology 2024Quote: ... cells stained with BV510-anti-TCRβ mAb and BV605-anti-CD4 mAb were fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (BioLegend, catalog# 424401) before staining with BV421-anti-T-bet mAb and PE-anti-GATA3 mAb.
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated on to 100 mm bacteriological Petri dish with complete DMEM/F12 and 20 ng/mL recombinant mouse macrophage colony stimulating factor protein (M-CSF) (Biolegend, Cat #576406). On day 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (Biolegend) per manufacturer’s instructions for HLA-A*02:01 ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Immunology 2024Quote: Bulk cultured T cells were stimulated with 5 µM peptides for 5hr in the presence of brefeldin A (Biolegend). The cells were collected and stained with specific antibodies against surface markers ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed once in PBS and then fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) before staining with PU.1-PE (BioLegend 7C2C34 cat #681307) or rat IgG2a-PE isotype (BioLegend RTK2758 ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... biotinylated NP peptide loaded SLA monomers were freshly assembled into tetramer with streptavidin BV421 or BV650 (both from Biolegend, UK). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 minutes at 37°C and tetramers added to the cells on ice for another 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured in stimulation media without peptide stimulation or with phorbol 12-myristate 13-acetate (PMA) and ionomycin (BioLegend), respectively ...
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bioRxiv - Immunology 2024Quote: ... 4×106 cells from spleen were plated in a 96-well plate and incubated with SIINFEKL peptide and anti-CD107a for 2 hours at 37°C before Brefeldin A (5.0 mg/mL: Biolegend, 420601) and Monensin protein transport inhibitors (4 pL/6E6 cells ...
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bioRxiv - Immunology 2024Quote: ... peptide-pulsed K562/HLA-E cells were stained with APC-labeled anti-human HLA-E mAb (clone 3D12, BioLegend, USA). Then the CD94/NKG2A+HLA-E+ and CD94/NKG2C+HLA-E+ K562/HLA-E cells were gated as targeted cells.
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bioRxiv - Immunology 2024Quote: ... derived from OT-1 mice treated with either CATR or vehicle were restimulated with 1µg/ml OVA-peptide for 6 hours and Brefeldin A (Biolegend, 420601) for 4hr prior to analysis.
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bioRxiv - Neuroscience 2022Quote: ... Antibodies (BioLegend) used were as follows:
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used were pericentrin (PCNT) antibody (PRB-432C-Biolegend, Covance Cat# PRB-432C ...
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bioRxiv - Genomics 2020Quote: ... oligonucleotide-conjugated antibodies and fluorescently labeled antibodies (Biolegend, USA) were both added at a 1:100 dilution in the same buffer as in the initial permeabilization step and incubated for 1h ...