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Citations for BioLegend :
351 - 400 of 645 citations for Biotin Rabbit Polyclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... ABCs were isolated from purified total B cells by CD11c positive selection using a biotin conjugated CD11c antibody (301612, Biolegend) and anti-biotin magnetic beads ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Immunology 2023Quote: ... CD8 (biotin, clone: 53-6.7), CD205 (clone: NLDC-145) and TNF-α(PE, clone: MP6-XT22) were purchased from BioLegend. The CD18-reactive mAb M18/2 was purchased from Biozym ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
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bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... non-natural killer cells were depleted by incubating the patient’s whole PBMCs sample with the biotin antibody cocktail (BioLegend 480053) followed by incubation with magnetic Streptavidin Nanobeads ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... were stained on ice for 25 minutes with the following panel of lineage-specific antibodies: Biotin-labeled TER-119 (TER-119, BioLegend), Biotin-labeled GR-1 (RB6-8C5 ...
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bioRxiv - Immunology 2023Quote: ... samples from animals that were vaccinated with the recombinant GPCysR4-I53-50A.30 To exclude I53-50A-binding B cells we included a SARS-CoV-1 Spike probe containing I53-50A-biotin which was labeled with streptavidin AF647 and streptavidin BV421 (both Biolegend). After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit α-keratin 10 and rabbit α-keratin 6 (Biolegend, San Diego, CA), rat α-keratin 8 (Developmental Studies Hybridoma Bank ...
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bioRxiv - Cell Biology 2021Quote: ... 25-4317-82) for HSC/MPP staining, or together with CD34-FITC (eBioscience, 11-0341-85) or CD34-biotin (BioLegend, 119304) and FcγR-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Molecular Biology 2020Quote: ... Linker oligo with 3’-terminal biotin was purchased from IDT (Coralville, Iowa) and purified streptavidin was purchased from BioLegend (Biolegend, #405150). Biotinylated antibody ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Immunology 2019Quote: The percentage of CAR and ΔLNGFR positive T cells was assessed by biotin-conjugated rhCD19-Fc (Cat # CD9-H5259, ACRO Biosystems, USA) with AF647-conjugated streptavidin (Cat # 405237, Biolegend, USA), and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining cells were incubated with Biotin anti-mouse Ly-6A/E (Sca-1) antibody (Biolegend; Cat. No. 122504; 1:75) at 4°C for 20 minutes then 10 µl Streptavidin beads (1:30 ...
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bioRxiv - Cell Biology 2022Quote: Cell suspensions from thymus were incubated with the biotin-conjugated anti-lineage marker antibodies described above together with CD4-APC (Biolegend 100411), CD8b-APC/Cy7 (Biolegend 126620) ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µL plasma was centrifuged at 10 000 g for 30 minutes and the total EV pellet washed once before resuspension with biotin-saturated MojoSort streptavidin magnetic nanobeads (Biolegend, USA) to deplete non-specific binding ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in these studies included chicken polyclonal anti-vimentin (Biolegend, Cat #919101, dilution 1:2000) and HRP-conjugated mouse monoclonal GAPDH (Proteintech ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-NMIIB Rabbit (Biolegend #909901) primary antibody was diluted 1:1000 in 5% milk TBST and placed on membrane overnight in a rocker platform at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... + donkey α-rabbit BV421 (Biolegend).
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-Pax6 (Biolegend, B214847), goat anti-phospho-Histone 3 (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-PCNT (Biolegend 923701), mouse anti-PLK1 (Thermofisher 33-1700) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti Pax6 (Biolegend 901301), Sheep anti TBR2/EOMES (R&D system AF6166) ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-Loricrin (905101, Biolegend) 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-Pax6 (Biolegend, 901301). The secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... KRT14 (Rabbit, 1:1000, BioLegend), KRT17 (Rabbit ...
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bioRxiv - Immunology 2020Quote: ... and rabbit anti-involucrin (BioLegend) antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit-β-Tubulin 3 (BioLegend) and mouse-STEM101 (Takara Bio) ...
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bioRxiv - Immunology 2022Quote: ... anti-rabbit PE (406421, Biolegend) and anti-mouse AF488 (A21202 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit keratin 10 (#905403 Biolegend), mouse keratin 14 (ab7800 Abcam) ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-Pax6 (901301, BioLegend), goat anti-FoxA2 (AF2400 ...
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bioRxiv - Immunology 2023Quote: ... Isotype control rabbit (Biolegend, #910801), rat (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... or Rabbit IgG isotype (BioLegend) to 60 µL of AminoLink™ Plus Coupling Resin (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: Fluorophore- or biotin-conjugated antibodies specific for human cell-surface antigens and cytokines were as follows: anti-CD71 (CY1G4, BioLegend, DF1513, NovusBio), anti-CD235a (HI264 ...
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bioRxiv - Immunology 2020Quote: ... OVA and HEL were biotinylated in house to ∼1 biotin per antigen molecule and tetramers constructed as described [14] using streptavidin-PE (Biolegend, CA, USA), streptavidin-APC (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... CD19+CD138+ plasma cells were isolated from PBMCs by positive selection using biotin conjugated CD138 antibody (352322, Biolegend, San Diego, CA, USA) and Easysep release human biotin positive selection cocktail (17653 ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Immunology 2024Quote: ... The CD11c-APC-depleted fraction was further incubated with anti-biotin beads (Milteniy Biotec, cat# 130-090-485) and streptavidin-PE/Cy7 (BioLegend, 1:500), followed by another MACS enrichment using MS columns ...
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bioRxiv - Immunology 2021Quote: ... Following a wash, cells were incubated (RT, 10 min) with Rhesus Fc Receptor Binding Polyclonal Antibody (Biolegend). Desired staining antibodies were added directly to this mixture at indicated dilutions (Supplemental Table 27 ...
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bioRxiv - Neuroscience 2020Quote: ... 50-μm brain sections were blocked with normal goat serum for 2 h and incubated overnight using biotin-conjugated anti-HA (Biolegend 901505, 1:500) and anti-parvalbumin (Synaptic Systems 195 004 ...
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bioRxiv - Neuroscience 2020Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...