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351 - 400 of 1379 citations for 4 Hydroxy 7 trifluoromethyl quinoline 3 carbohydrazide since 2020

• Expression of Tim-3 drives naïve Treg to an effector-like state with enhanced suppressive activity

  Hridesh Banerjee, et al., bioRxiv - Immunology 2020

  Quote: ... CTLA-4 clone UC10-4B9 (BioLegend), CD103 clone M290 (BD) ...
• Acute lymph node slices are a functional model system to study immunity ex vivo

  Maura C. Belanger, et al., bioRxiv - Immunology 2020

  Quote: ... IL-4 (ELISA MAX standard, Biolegend) and TNFα (R&D Systems ...
• CTLA-4 tail fusion enhances CAR-T anti-tumor immunity

  Xiaoyu Zhou, et al., bioRxiv - Immunology 2023

  Quote: ... or anti-CTLA-4 antibody (Biolegend) at 37°C for 1 hour ...
• Selective Inhibition of Interleukin-2 Inducible T Cell Kinase (ITK) Enhances Anti-Tumor Immunity in Association with Th1-skewing, Cytotoxic T cell Activation, and Reduced T Cell Exhaustion

  Lih-Yun Hsu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-human IL-4 (OA19A66, Biolegend) and anti-human IL-5 (TRFK5 ...
• Human cerebrospinal fluid single exosomes in Parkinson’s and Alzheimer’s diseases

  Koya Yakabi, et al., bioRxiv - Neuroscience 2023

  Quote: ... Aquaporin-4 (E5206) (BioLegend catalog #600352), CD11b (M1/70 ...
• A single-dose MCMV-based vaccine elicits long-lasting immune protection in mice against distinct SARS-CoV-2 variants

  Kristin Metzdorf, et al., bioRxiv - Immunology 2024

  Quote: ... CD11a (M17/4, BioLegend, CA, USA), CD62L (MEL-14 ...
• Soquelitinib, A Selective Inhibitor of Interleukin-2-Inducible T Cell Kinase (ITK), is Active in Several Murine Models of T Cell-Mediated Inflammatory Disease

  Lih-Yun Hsu, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IL-4 (11B11, Biolegend) and anti-mouse IFNγ (XMG1.2 ...
• Membrane positioning across antigen-induced synaptic contacts tunes CAR-T cell signaling and effector responses

  Fenglei Li, Kaushik Choudhuri, bioRxiv - Immunology 2023

  Quote: ... with 100ng/ml hIL-4 (Biolegend) 2days post transduction ...
• Lyophilized mRNA-lipid nanoparticle vaccines with long-term stability and high antigenicity against SARS-CoV-2

  Liangxia Ai, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 PE (Biolegend, cat.504104), and IL-5 APC (Biolegend ...
• PPAR-γ regulates the effector function of human T_H9 cells by promoting glycolysis

  Nicole L. Bertschi, et al., bioRxiv - Immunology 2022

  Quote: ... IL-4 (50 ng/ml) (BioLegend) for TH2 cells ...
• Diet Induced Obesity and Diabetes Enhance Mortality and Reduces Vaccine Efficacy for SARS-CoV-2

  Robert M Johnson, et al., bioRxiv - Microbiology 2023

  Quote: ... IL-4 BV605 (BioLegend, clone: 11B11), and IL-17a BV711 (BioLegend ...
• Initial TCR Signal Strength Imprints GATA3 and Tbet Expression Driving T-helper Cell Fate Decisions

  Mohammad Ameen Al-Aghbar, et al., bioRxiv - Immunology 2024

  Quote: ... PE/Cy7-IL-4 (11B11, Biolegend), PerCP-Cy5.5-IFNγ (XMGL2 ...
• IL-1β Signalling Modulates T Follicular Helper and Regulatory Cells in Human Lymphoid Tissues

  Romain Vaineau, et al., bioRxiv - Immunology 2024

  Quote: ... CTLA-4 (BV605, 1/40, BioLegend), PD-1 (PE-Cy5.5 ...
• Limiting mitochondrial-derived ATP transfer to the cytosol enhances T-cell activation

  Omri Yosef, et al., bioRxiv - Immunology 2024

  Quote: ... and IL-4 (Biolegend, BLG-431104) cytokines using enzyme-linked immunosorbent assay (ELISA).
• Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia

  Lawrence J. Dooling, et al., bioRxiv - Bioengineering 2024

  Quote: ... or interleukin-4 (IL4, BioLegend 574302) to polarize to M1 and M2 states ...
• Non-productive exposure of PBMCs to SARS-CoV-2 induces cell-intrinsic innate immunity responses

  Julia Kazmierski, et al., bioRxiv - Immunology 2022

  Quote: ... and anti-TIM-3/CD366-FITC (#345022; Biolegend). To determine ACE2 cell surface expression ...
• Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies

  Yunlong Cao, et al., bioRxiv - Immunology 2021

  Quote: ... 3 μL PE/Cyanine7 anti-IgM(Biolegend, 314532), and fluorophore-labelled Receptor-Binding Domain (RBD ...
• Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies

  Yunlong Cao, et al., bioRxiv - Immunology 2021

  Quote: ... 3 μL FITC anti-CD20 Antibody (Biolegend, 302304), 3.5 μL Brilliant Violet 421 anti-CD27 Antibody (Biolegend ...
• Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies

  Yunlong Cao, et al., bioRxiv - Immunology 2021

  Quote: ... 3 μL FITC anti-CD19 Antibody (Biolegend, 392508), 3 μL FITC anti-CD20 Antibody (Biolegend ...
• In vivo analysis of Trypanosoma cruzi persistence foci at single cell resolution

  Alexander I. Ward, et al., bioRxiv - Microbiology 2020

  Quote: ... Antibodies against β-tubulin-3 (Biolegend, Cat#802001), CD45 (Tonbo Biosciences ...
• A P2rx7 passenger mutation affects the vitality and function of immune cells in P2X4ko and other transgenic mice

  Marco Er-Lukowiak, et al., bioRxiv - Immunology 2020

  Quote: ... and recombinant IL-3 (10 ng/ml, Biolegend). BMMCs were transferred into a new petri dish with new BMMC medium every 3-4 days ...
• Overcoming microenvironment-mediated chemoprotection through stromal galectin-3 inhibition in acute lymphoblastic leukemia

  Somayeh S. Tarighat, et al., bioRxiv - Cancer Biology 2021

  Quote: ... and galectin-3 (#125402, Biolegend, San Diego, CA), were diluted 1:1000 or 1:2000 in 5% BSA and incubated for 1hour at room temperature ...
• Systematic functional screening of chromatin factors identifies strong lineage and disease dependencies in normal and malignant haematopoiesis

  D Lara-Astiaso, et al., bioRxiv - Genomics 2022

  Quote: ... and CD55 (PE-conjugated; clone RIKO-3; Biolegend). Percent Gran-like (CD11b-high/Gr-1+ ...
• Rewiring of the TCR signalosome in natural intestinal Intraepithelial T lymphocytes drives non-deletional tolerance

  Harriet J. Watt, et al., bioRxiv - Immunology 2023

  Quote: ... LAG-3-PE-Cy7 (1:200) (all Biolegend) and TCRβ-BV786 (1:200 ...
• Type I interferons sensitise HIV-1-reactivating T-cells for NK cell-mediated elimination despite HDACi-imposed dysregulation of innate immunity

  Julia Prigann, et al., bioRxiv - Microbiology 2023

  Quote: ... and anti-TIM-3/CD366-FITC (#345022; Biolegend). For intracellular immunostaining ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and propidium iodide (final concentration 3 μM; Biolegend) was used for dead cell exclusion ...
• Rapid activation of IL-2 receptor signaling by CD301b⁺ DC-derived IL-2 dictates the outcome of helper T cell differentiation

  Naoya Tatsumi, et al., bioRxiv - Immunology 2023

  Quote: ... and GATA-3 (16E10A23) were purchased from BioLegend. Anti-pSTAT5 (Tyr694 ...
• Therapy-induced senescence upregulates antigen presentation machinery and triggers anti-tumor immunity in Acute Myeloid Leukemia

  Diego Gilioli, et al., bioRxiv - Cancer Biology 2022

  Quote: ... CD223 (LAG-3) BV605 (cat#369324 from BioLegend), CD3 BUV737 (cat#565466 from BD) ...
• Therapy-induced senescence upregulates antigen presentation machinery and triggers anti-tumor immunity in Acute Myeloid Leukemia

  Diego Gilioli, et al., bioRxiv - Cancer Biology 2022

  Quote: ... CD366 (TIM-3) BV421 (cat#345008 from BioLegend), CD223 (LAG-3 ...
• TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring gene

  Evelyn Kabirova, et al., bioRxiv - Genetics 2022

  Quote: ... and mrIL-3 (10 ng/ml) (Biolegend, 575502). Mast cells were also stained by toluidine blue (Biovitrum ...
• NRF2-dependent regulation of the prostacyclin receptor PTGIR drives CD8 T cell exhaustion

  Michael S. Dahabieh, et al., bioRxiv - Immunology 2024

  Quote: ... TIM-3 BV711 (#119727, Clone RMT3-23, Biolegend), PD-1 BUV737 (#749306 ...
• Increasing Signal Intensity of Fluorescent Oligo-Labeled Antibodies to Enable Combination Multiplexing

  Madeline E. McCarthy, et al., bioRxiv - Molecular Biology 2024

  Quote: ... (3) CD4 (clone RPA-T4; Biolegend Cat: 300502) labeled with Atto488/647 int ...
• HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

  Amber D Jones, et al., bioRxiv - Immunology 2024

  Quote: ... and/or anti-Tim-3 (CD366) antibody (BioLegend, UltraLEAF purified anti-human ...
• PD-1 blockade enhances HIV-1 vaccine-induced CD8⁺ T-cell responses in PWH early ART-treated

  Miguel Marin, et al., bioRxiv - Immunology 2024

  Quote: ... and TIM-3 (PE, clone F38-2E2, Biolegend) antibodies for 25 minutes ...
• Divergent response to radio-immunotherapy is defined by intrinsic features of the tumor microenvironment

  Jacob Gadwa, et al., bioRxiv - Immunology 2024

  Quote: ... GATA-3: Alexa Fluor 647 (Clone: 16E10A23, Biolegend). Samples were analyzed on a Cytek Aurora spectral cytometer (Cytek Biosciences) ...
• Enhancing and inhibitory motifs have coevolved to regulate CD4 activity

  Mark S. Lee, et al., bioRxiv - Immunology 2021

  Quote: ... were coated overnight at 4°C with 8µg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
• Proteomics reveals unique identities of human TGF-β-induced and thymus-derived CD4⁺ regulatory T cells

  Mark Mensink, et al., bioRxiv - Immunology 2022

  Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...
• The CD4 transmembrane GGXXG and juxtamembrane (C/F)CV+C motifs mediate pMHCII-specific signaling independently of CD4-LCK interactions

  Mark S. Lee, et al., bioRxiv - Immunology 2023

  Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...
• Mouse Memory CD8 T cell subsets defined by Tissue Resident Memory (T_RM) Integrin Expression Exhibit Distinct Metabolic Profiles

  Mike Sportiello, et al., bioRxiv - Immunology 2023

  Quote: ... and plated in TRM-like culture media with 10 ng/mL mouse IL-7 (Biolegend Catalog No. 577804) and 10 ng/mL human TGFβ1 (Fisher Scientific PHG9214 ...
• TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration

  Hangu Nam, et al., bioRxiv - Bioengineering 2023

  Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
• A Gallium-68-Labeled Peptide Radiotracer For CD38-Targeted Imaging In Multiple Myeloma With PET

  Ajay Kumar Sharma, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...
• Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineering

  Keqiang Xie, et al., bioRxiv - Bioengineering 2022

  Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
• Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein

  Darko Bosnakovski, et al., bioRxiv - Molecular Biology 2023

  Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
• ALDH1A3-acetaldehyde metabolism potentiates transcriptional heterogeneity in melanoma

  Yuting Lu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... This was centrifuged again (300xg for 7 minutes) and resuspended in 1X red blood cell lysis buffer (BioLegend) in deionized water ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... the Siglec+ population was sorted by staining the cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
• Catalytic inhibition of KAT6/KAT7 enhances the efficacy and overcomes primary and acquired resistance to Menin inhibitors in MLL leukaemia

  Shellaina J. V. Gordon, et al., bioRxiv - Cancer Biology 2024

  Quote: ... After 7-days treatment cells were stained with anti-CD11b antibody (Clone M1/70, Biolegend, Cat. 101212, RRID:AB_312794) and DAPI before analysis by flow cytometry ...
• Dietary Protection Against the Visual and Motor Deficits Induced by Experimental Autoimmune Encephalomyelitis

  Katarzyna Zyla-Jackson, et al., bioRxiv - Immunology 2023

  Quote: ... we incubated sequential de-paraffinized sections with anti-2’,3’-cyclic-nucleotide 3’-phosphodiesterase (CNPase) (mouse CNPase: 1:200 dilution; Biolegend, Inc.), anti-CD3 (rabbit ...
• Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

  Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...
• Macrophage-Based iNos Reporter Reveals Polarization and Reprogramming in the Context of Breast Cancer

  Javier A. Mas-Rosario, et al., bioRxiv - Immunology 2022

  Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...
• Progenitor identification and SARS-CoV-2 infection in long-term human distal lung organoid cultures

  Ameen A. Salahudeen, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti-TNFRSF12A (clone ITEM-4, Biolegend) was utilized for (Fig ...