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Citations for BioLegend :
351 - 400 of 7156 citations for 3 Ethyl 1 methyl 1H imidazolium salt with propanedinitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Cell Biology 2024Quote: ... the sperm smears were fixed for 10 min with 3.2 % paraformaldehyde and permeabilized with the Intracellular Staining Perm Wash Buffer for 3 x 5 min (421002, BioLegend, USA). Following permeabilization ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31; LFA-3: Biolegend, cloneTS2/9 ...
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bioRxiv - Cancer Biology 2024Quote: ... Residual red blood cells (RBC) were lysed incubating cell suspension for 3 minutes at 4°C with 1X RBC Lysis Buffer (BioLegend). Cell suspensions were washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... bone marrow progenitor cells were harvested from the femur of C57BL/6 mice and plated at a density of 3×106/3mL well on 6-well plates with 20 ng/mL GM-CSF (BioLegend) in RPMI 1640 medium (Wisent ...
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bioRxiv - Immunology 2024Quote: ... bone marrow cells were plated at 3×105/mL density in IMDM medium containing 20 ng/mL mouse GM-CSF (BioLegend) and cultured for 7-9 days.
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bioRxiv - Immunology 2024Quote: ... incubated for 4 minutes at room temperature, centrifuged (3 min, 500g, 4C) and re-suspended with FACS buffer containing CD16/CD32 blocking antibodies (BioLegend) for 15 minutes on ice ...
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bioRxiv - Genomics 2024Quote: ... Cells were then cultured for 12-18h in DMEM/10%FBS supplemented with 100ng/mL mSCF/mIL-3/mIL-6 (BioLegend) at 37°C at 5% CO2 [Step Four] ...
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bioRxiv - Cancer Biology 2022Quote: ... CD106 (1:100, MVCAM.A, #105717), CD62L (1:200, MEL-14, #104435), PD-1 (1:100, 29F.1A12, #135215) (Biolegend); Streptavidin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Immunology 2023Quote: ... 1:100), GR1 (108428, 1:300), NK1.1 (108728, 1:200), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, IL-22 (12-7221-82 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD11b (AF594, 1:200), CD14 (PE-Cy5, 1:150), CD204 (PE-Cy7, 1:100) and Zombie NIR (1:1000) (Biolegend); CTY was captured on the PE channel.
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with biotinylated anti-mouse antibodies (CD31, 1:150, BioLegend; CD45, 1:150, BioLegend; and Sca-1, 1:150, BioLegend) at 4°C for 40 min ...
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bioRxiv - Cell Biology 2021Quote: ... PAC-1 FITC (1:12, D Bioscience) and annexin V APC (1:60, Biolegend) for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... TGM-1 (SigmaAldrich®, HPA040171, 1:150) and CD31 (BioLegend®, 303126, 1:100). Secondary antibodies were also applied overnight in a 3% BSA solution in PBS frequently alongside rhodamine-phalloidin and DAPI ...
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bioRxiv - Immunology 2022Quote: ... PD-1 (1:50; clone 29F.1A12; BioLegend); Cxcr5 (1:50 ...
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bioRxiv - Physiology 2022Quote: ... rat anti-ICAM-1 (BioLegend 116102, 1:100), mouse anti-αSMA-Cy3 (Sigma C6198 ...
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bioRxiv - Cell Biology 2020Quote: ... and -Mac-1 (M1/70, BioLegend, 1:100).
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bioRxiv - Immunology 2021Quote: ... anti-CX3CR1 (Clone 2A9-1, 1:50 BioLegend); anti-CCR2 (Clone K036C2 ...
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bioRxiv - Systems Biology 2023Quote: ... GR-1 (BioLegend, clone: RB6-8C5, 1:100), F4/80 (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... CD19-FITC (Biolegend, clone MB19-1, 1:100), CD45-V450 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... CD86 (BV650, Biolegend, clone: GL-1, 1:400), CD80 (APC ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-Pu.1 (1:500, Biolegend, 681302); guinea pig anti-s100b (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sca-1 (rat, 1:100 Alexa488-conjugated, BioLegend),Tyrosine Hydroxylase (sheep ...
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bioRxiv - Neuroscience 2023Quote: ... 1-16 (clone 6E10) (Biolegend, 803013, 1:200).
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bioRxiv - Immunology 2022Quote: ... CD48 (Pacific Blue, HM48-1, Biolegend, 1/100), CD16/32 (PercPCy5.5 ...
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NR2F2 Reactivation in Early-life Adipocyte Stem-like Cells Rescues Adipocyte Mitochondrial OxidationbioRxiv - Physiology 2024Quote: ... Sca-1 BV510 at 1:200 (BioLegend, 108129), and CD24 PE at 1:100 (BioLegend ...
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bioRxiv - Biochemistry 2024Quote: ... 1:500 and 4G8 (1:1000; Biolegend 800702) overnight at room temp ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse IgG2b anti-MART1 (1:1, Biolegend, 917902). Sections were then treated with 0.1% Sudan Black (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...