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Citations for BioLegend :
3901 - 3950 of 4293 citations for Mouse AWAT2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cytokine concentration in these samples were determined using CBA mouse Inflammation Kit (BDTM) or ELISA according to the manufacturers’ instructions (Biolegend).
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bioRxiv - Immunology 2024Quote: ... cells were washed again and stained with anti-mouse antibodies to intracellular markers anti-TNF-α-Alexa Flour 700 (Biolegend, Alexa Fluor® 700 anti-mouse TNF-α Antibody ...
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bioRxiv - Immunology 2024Quote: ... and anti-dsDNA antibodies were detected with a 1/1000 dilution of alkaline phosphatase–conjugated goat anti-mouse IgG (BioLegend) for 1 hour at 37°C and developed with a phosphatase substrate for 30 min at 37°C.
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bioRxiv - Immunology 2024Quote: ... Mouse anti-human HLA-E (clone 4D12) primary antibody (MBL Lifescience) and then secondary antibody goat anti-mouse conjugated with APC (BioLegend) were applied with staining buffer (DPBS ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
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bioRxiv - Cell Biology 2023Quote: ... and cultured in DMEM/F-12 medium containing 10% FBS and 20 ng/mL mouse macrophage colony-stimulating factor (BioLegend) for 7 days ...
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bioRxiv - Microbiology 2024Quote: ... The amount of Ag-specific IgG was then quantitated by running a western blot using mouse IgG (BioLegend CAT#944803) of known mass as a standard ...
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Pathology 2023Quote: ... followed by the surface monoclonal mouse anti-human conjugated antibodies: anti-CD15-PE (clone H198) and anti-CD11b-FITC (ICRF44) from Biolegend. Intracellular staining was performed after fixation and permeabilization with Fix/Perm kit (eBiosciences) ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were re-suspended in flow cytometry buffer and incubated with TruStain FeX™ PLUS anti-mouse CD16/32 (BioLegend) (0.25 μg per 106 cells in 100 μL ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Genomics 2023Quote: ... control samples (Mouse IgG2a) were sorted on the basis of live single cells that are CD69− (PE-Dazzle 594, Biolegend) and the stimulated samples (anti-CD3 plus either anti-CD28 ...
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bioRxiv - Neuroscience 2023Quote: ... were used to confirm results with McSA1: a mouse monoclonal antibody to Aβ residues 1-16 (1:1000; clone 6E10; Cat#803001, Biolegend) or a mouse monoclonal antibody to Aβ residues 17-24 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The phenotype of differentiated HL-60 cells was assessed by flow cytometry using mouse antihuman CD35 PE conjugated antibody (BioLegend) and mouse anti-human CD71 APC conjugated antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... 5000 cells of each compartment were labeled with anti-mouse hashtag oligonucleotide (HTO) antibodies (TotalSeq™-C0301/C0302/C0303, Biolegend) and pooled together ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated O/N at 4°C with a mouse anti-iNOS2 IgG antibody labeled with Alexa Fluor 564 (BioLegend)) or a mouse anti-ARG-1 IgG antibody labeled with FITC (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... Adjacent sections were deparaffinized in citrate and stained with monoclonal mouse anti-F4/80 antibody at a 1:400 dilution (BioLegend), or polyclonal goat anti-MPO antibody at a 1:500 dilution (R&D Systems) ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were first blocked with 2% mouse serum (eBioscience San Diego, California) and Human Fc Block (Biolegend San Diego, California) for 10 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... whereas binding of T cell-secreted A4 monomer on OT-I T cell CD47 was assessed indirectly by staining with anti-mouse CD47 (miap301, Biolegend) at 4°C for 30min.
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bioRxiv - Microbiology 2023Quote: ... samples were stained with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with HBSS (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the slides were incubated for 1 hour in the dark at room temperature with an Alexa 488-conjugated goat anti-mouse IgG antibody (BioLegend). Following three washing steps ...
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bioRxiv - Immunology 2023Quote: ... stained with a mixture of fluorochrome-conjugated antibodies (1:200 FITC anti-mouse/human CD11b M1/70, BioLegend, California, USA), (1:200 APC/Cyanine7 anti-mouse F4/80 ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Physiology 2023Quote: ... Non-cardiomyocytes resuspended in Cell Staining Buffer (phosphate-buffered saline containing 2% FBS and 0.1% sodium azide) were treated with anti-mouse CD16/CD32 antibody (BioLegend, #101302) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: Staining for flow cytometry was done using the following anti-mouse mAbs: CD3 (17A2) conjugated to PE/Dazzle 594 (BioLegend), CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... The antibody solution was then removed and the isolated lymph node cells were added at 2×105 cells per well together with soluable anti-mouse CD28 (102112, Biolegend) at a final concentration of 2 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies, rabbit anti-phospho-cofilin (CST, #3311, 1:100) and mouse anti-SMI-32 (BioLegend, # SMI-32R, 1:250), were applied in blocking buffer at 4°C overnight ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Bioengineering 2023Quote: ... PE anti-mouse CD19 antibody (Catalog No. 115508) and PC anti-mouse/human CD45R/B220 antibody (Catalog No. 103212) were acquired from BioLegend. MIK665 was from Selleck Chem ...
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bioRxiv - Cancer Biology 2023Quote: ... EMT phenotype of in vivo isolated tumor cells was analyzed using the same antibody panel implemented with anti-mouse CD45 (30-F11; Biolegend), CD90.1 (OX-7 ...
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bioRxiv - Cancer Biology 2023Quote: ... The FCM panel used to analyze EMT phenotype of in vitro tumor cells relied on the use of the anti-mouse pancytokeratins (C-11; Biolegend), CD73 (TY/11.8 ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: Antibodies were purchased from the following sources: mouse monoclonal antibody against the HA11 epitope (16B12) were from BioLegend (mms-101p), rat monoclonal antibody against the N-terminus of DAT (MAB369 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-V5 (BLD-680605, clone 7/4) and rabbit polyclonal anti-V5 (BLD-903801) were obtained from BioLegend. Mouse monoclonal anti-ICP36 (anti-UL44 ...
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bioRxiv - Immunology 2023Quote: ... cells were hashtagged with mouse TotalSeqA DNA-barcoded hashtag antibodies at the same time as staining with fluorophore-conjugated antibodies (BioLegend). Cells were sorted into RPMI + 5% FCS in DNA Lo-Bind tubes (Eppendorf ...
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bioRxiv - Immunology 2023Quote: ... cells were hashtagged with mouse TotalSeqA DNA-barcoded hashtag antibodies at the same time as staining with fluorophore-conjugated antibodies (BioLegend). Hashtags used in each experiment are provided in Table S1 ...