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Citations for BioLegend :
3851 - 3900 of 5409 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Jurkat cells were labelled with 25 μg/ml anti-human CD3 antibody (BioLegend, 317304, clone OKT3) and 3 μg/ml recombinant annexin V (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... The cells were then washed in cold PBS and resuspended in RBC lysis buffer (BioLegend, 420301) for 1 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The resuspended cell pellet was stained for anti-mouse CD3-PE (1:200, 145-2C11 Biolegend), anti-mouse CD45-APC/Cy7 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were either first stained with either Zombie UV or Zombie NIR fixable viability dyes (BioLegend) (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... in B cell medium in the presence or absence of 10 μg/ml anti-CD86 (BioLegend) or 1 μg/ml CD40L (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated in PBS containing Zombie UV™ Fixable Viability Dye (BioLegend, San Diego, CA) at 4 °C for 20 min.
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bioRxiv - Cancer Biology 2022Quote: ... B cell depletion was done by a monthly intravenous injection of anti-CD20 (Clone SA271G2, BioLegend), starting one week prior to orthotopic transplantation of cells.
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bioRxiv - Developmental Biology 2022Quote: ... These suspended single cells were stained with Ter119-Alexafluor-647 (Biolegend, Catalog# 116218, 1:100 dilution) for 30 minutes at 4° Celsius ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for cell sorting experiments: anti-CD49f-APC (1:300, 313615, BioLegend), anti-CD49f-PercP/Cy5.5 (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for cell cycle analysis: anti-CD49f-FITC (1:200, 313606, BioLegend), anti-Sca1-Ly-6A/E-Violet 605 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... lysed for 30sec at room temperature in red blood cell lysis buffer (Biolegend, catalog no. 420301), washed with ice-cold PBS-EDTA and centrifuged at 1300rpm for 10min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Negative cells were sorted based on the absence of B2M-PE antibody staining (clone 2M2, BioLegend) using magnetic separation with PE-beads (Miltenyi) ...
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bioRxiv - Developmental Biology 2022Quote: ... live GFP+ cells (Extended Data Fig.1; live stain 1:500 propidium iodide solution (BioLegend #421301)) were sorted on an FACSAria III (BD ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were treated with CD16/32 Fc blocking antibodies (Biolegend; catalog 101310; clone 93) to prevent non-specific antibody binding and stained with the fluorescent-labeled antibodies for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... cells were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (Biolegend) for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were filtered through a 70 µm strainer and stained with the following antibodies from Biolegend: α6-integrin-PE (1:1,000) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained for 15 min in the dark with 5 μL APC Annexin V (Biolegend) and 5 μL 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... CAR CD8+ T cells were identified with CD3-APC/Cy7 or CD8-Alexa 700 antibodies (Biolegend). Activation of CAR-MAIT and CAR CD8+ T cells were determined with CD25 staining using CD25-APC antibody (Biolegend) ...
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bioRxiv - Neuroscience 2022Quote: ... red blood cells (RBCs) were removed by lysis in 2 mL of RBC lysis buffer (BioLegend) for 3 minutes ...
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bioRxiv - Immunology 2022Quote: ... Isolated cells were stained with Zombie NIR and CD4 Pacific Blue (both Biolegend, San Diego, USA) and were acquired on BD LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated using Dynabeads M-280 Tosylactivated magnetic beads coated with αCD3 (clone OKT3, Biolegend) and either αCD28 (clone CD28.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Immunology 2022Quote: ... Colon cells were either untreated or treated with 25 µg/ml recombinant mouse IFN-β1 (Biolegend.) Supernatant was collected for ELISA and cells were stained for flow.
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Cells were counted and stained with anti-mouse Epcam CD326-AlexaFluor647 antibody (1:100, Biolegend, 118212), CD31-APC (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... PI-labeled cells were stained with Ki-67-APC antibody (Biolegend, San Diego, CA, USA, # 652406) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with 1 µM carboxyfluorescein succinimidyl ester (CFSE) dye (BioLegend, San Diego, CA, USA) for 15 minutes under standard cell culture conditions ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were stained with 2.5 µM Violet Tag-It dye (BioLegend, San Diego, CA, USA) for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The following cell-surface markers were used in the experiment: EpCAM-PE (BioLegend, 118206, 1:200), CD49f-FITC (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells expressing IgE were assessed using anti-Mouse IgE PE (1:200, BioLegend, 406908; RME-1). Live/ dead cells were discriminated after staining with Zombie Aqua viability dye ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed for 20 minutes at room temperature using a Fixation Buffer (Biolegend, Cat: 420801) followed by permeabilization (Biolegend ...
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Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: ... dead cells were excluded from the analysis using Near-IR Live/Dead fixable staining reagent (BioLegend). Doublets were excluded by FSC-Area versus FSC-Height gating.
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bioRxiv - Genomics 2024Quote: ... Each cell pellet was resuspended in 100μL of 1ξDPBS with 1:500 Zombie Violet (BioLegend 423113) and incubated for 20 mins at room temperature in the dark ...
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bioRxiv - Genomics 2024Quote: ... then resuspended in 105μL of cell staining buffer with 1:21 Human TruStain FcX (BioLegend 422301), followed by incubation at 4°C for 20 mins at 300rpm ...
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bioRxiv - Developmental Biology 2024Quote: ... 500 000 cells were incubated with 1ul TotalSeq Antibody (HTO) (BioLegend, Catalog No. 155863 and 155861) in 50 μl FACS buffer for 30 min on ice ...
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bioRxiv - Immunology 2024Quote: ... the cells were harvested and stained with CD39-perCP-eF710 (Clone 24DMS1, BioLegend 5015962, 1:100), CD8b-Alexa 647 (Clone YTS1567.7 ...
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bioRxiv - Immunology 2024Quote: ... using the MojoSort magnet with MojoSort Mouse CD4 Naïve T Cell Isolation Kit (Cat.#480040 BioLegend). T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... one million cells were first resuspended in100LμL buffer containing 5LμL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15Lminutes ...
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bioRxiv - Immunology 2024Quote: Isolated cell suspensions were washed with PBS and stained for viability with ZombieUV (1:2000; Biolegend). Samples were then blocked with 5 μg/ml αCD16/CD32 (2.4G2 ...
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bioRxiv - Immunology 2024Quote: ... Then the cells were stained in PBS containing 1:1000 diluted Zombie NIR viability dye (BioLegend) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions were labelled with TotalSeqA anti-human hashtag oligonucleotide (HTO) antibodies (Biolegend, 1:50) to allow downstream de-multiplexing in silico as previously described9 ...
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bioRxiv - Cancer Biology 2023Quote: ... The magnetically labeled fraction (non-NK cell population) was retained using a magnetic separator (BioLegend 480019). The untouched NK cells (CD56+ ...
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bioRxiv - Immunology 2023Quote: Cells were stained with antibodies against murine CD45 (30-F11, Biolegend cat. no. 103112, RRID: AB_312976), CD45.1 (A20 ...
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bioRxiv - Cell Biology 2023Quote: ... Zombie Red viability dye was used to distinguish live and dead cells (Biolegend, San Diego, CA). Fc receptors were blocked with anti-CD16/32 in the antibody staining solutions (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended 0.04% BSA in PBS and stained with a viability marker (Zombie NIR, BioLegend) and Fc block (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed and incubated with TruStain fcX (CD16/CD32, Clone 93, BioLegend, catalog # 101320) to prevent non-specific binding ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions were incubated for FcR block (TruStain FcX, anti-mouse CD16/32, 101320, BioLegend) in 0.5% BSA/PBS buffer for 20 min on ice ...
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bioRxiv - Immunology 2023Quote: Cells were harvested and surface stained with the following antibodies: CD57-FITC (HNK-1) from BioLegend; KIR3DL1S1-APC (Z27.3.7) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then resuspended in FACS buffer with 1 μg/ml propidium iodide (PI) (BioLegend, 421301). FACS sorting was performed on a BD FACS Aria II sorter using a 100-µm nozzle ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (BioLegend 424401) following the manufacturers instructions and were then stained with anti-FOXP3 APC (Invi-trogen 17-5773-82 ...