Labshake search
Citations for BioLegend :
3801 - 3850 of 7647 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: The following antibodies were used: unconjugated anti-CD16/CD32 (1 μg per 1×106 cells; clone 93; BioLegend Cat. No. 101320), H2-Dd-FITC (1:200 ...
-
bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
-
bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... was first applied for 1 h followed by staining with PE anti-streptavidin (1:200, Biolegend, cat. no. 410503, clone 3A20.2) and anti-c-myc-FITC (1:50 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and APC/Fire 750 conjugated anti E-Cadherin (dilution: 1:100; clone DECMA-1. All antibodies purchase from BioLegend (CA, USA). Samples were washed in 1mL 2% FBS and PBS and centrifuged at 2000rpm for 5mins ...
-
bioRxiv - Immunology 2023Quote: ... Stained cells and “fluorescence minus one” (FMO) controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (BioLegend, #424001) as a live/dead stain ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked in 5% NDS in TBS for 1 hour before being incubated with 1:1000 6E10 (BioLegend 803004) overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... PE-anti-PD-1 (clone RMP1-30, 1:20) and its isotype control PE-Rat IgG2b,κ (clone RTK4530, 1:20) were purchased from Biolegend. PE-anti-PD-L1 (clone MIH5 ...
-
bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
-
bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
-
bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
-
bioRxiv - Neuroscience 2023Quote: ... CD3-APC-Cy5.5 (1:40, Abcore #AC12-0212-04), CD4-PerCP-eFluor710 (1:40, eBioscience #46-0040-82) and CD8a-PE (1:40, BioLegend #200607) antibodies in FACS buffer with 2 µg DAPI to distinguish cells from debris ...
-
bioRxiv - Molecular Biology 2024Quote: ... and resuspended in a cocktail of FACS buffer with anti-FCR (made in house; 1:10) and fluorochrome-conjugated antibodies against B220 (RA3-6B2-BV605; 1:200; BioLegend #103244), TCRβ (H57-597-PE-Cy7 ...
-
bioRxiv - Neuroscience 2024Quote: ... CD3-APC-Cy5.5 (1:40, Abcore #AC12-0212-04), CD4-PerCP-eFluor710 (1:40, eBioscience #46-0040-82) and CD8a-PE (1:40, BioLegend #200607) antibodies in FACS buffer with 2 µg of DAPI to distinguish cells from debris for 1 h at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... 1% normal donkey serum and 0.3% Triton X-100) and incubated in primary antibodies against FOX3 (NeuN, BioLegend, 834501, 1:1000), OLIG2 (R&D ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell were blocked with 1% BSA in PBS for 1h and then incubated with anti-yH2AX-Alexa Fluor 647 antibody (1:200) (Biolegend, 613408) or Anti PCNA-PE conjugate (1:200 ...
-
bioRxiv - Immunology 2024Quote: ... and resuspended in FACS buffer (1 x PBS supplemented with 0.5% BSA) containing purified anti mouse CD16/32 (Clone: 93, BioLegend, 1:50) and incubated at 4 °C for 20 min in the dark ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were activated for two days at 1×106 cells/mL on tissue-culture plates with 1 μg/μl of surface-bound anti-CD3 (BioLegend, 317302) and anti-CD28-antibodies (BioLegend ...
-
bioRxiv - Immunology 2024Quote: ... Spleen and PEC samples were diluted to 1:20 and 1:40 and analyzed for IL-12p40 by ELISA (BioLegend 431604) per the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: ... for 1 h and probed with primary antibody at 4 °C overnight with 1:500 anti-His-tag antibody (BioLegend, J099B12) and 1:200 anti-CD95L antibody (Abnova ...
-
bioRxiv - Cancer Biology 2024Quote: ... PE/Dazzle594 anti-PD-1/CD279 (clone 29F.1A12, Cat. 135228, 1:200) and TruStain FcX™ (anti CD32/16 antibody, 1:50) were from BioLegend. PE anti-CD38 (clone 90/CD38 ...
-
bioRxiv - Developmental Biology 2024Quote: ... They were incubated for overnight at 4° with primary antibodies to rabbit anti-α-tubulin (1:200; 11H10, cell Signaling Technology) or rat anti-mouse FR4 (JUNO; 1:250; 12A5 BioLegend), wash 3 times in 1% BSA/PBS/PVA ...
-
bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 2 million cells per sample were resuspended in CITE-seq staining buffer (2% BSA, .01% Tween in PBS) and incubated for 10 minutes with Fc receptor block (TruStain FcX, BioLegend and FcR blocking reagent, Miltenyi) to prevent antibody binding to Fc receptors ...
-
bioRxiv - Cancer Biology 2021Quote: ... tissues were incubated overnight at room temperature with 1 μg.ml−1 DAPI or 0.5 μM Sytox™ Blue solution (Biolegend, catalog no. 425305) to counterstain cell nuclei and mounted using Vectashield mounting media (Vector Laboratories ...
-
bioRxiv - Cell Biology 2020Quote: ... The following FACS antibodies were used at 1:200: mouse anti-human CD45 APC-Cy7 (BioLegend; Cat#304014; used at 1:200), mouse anti-human anti-CD11b-APC-Cy7 (BD ...
-
bioRxiv - Immunology 2020Quote: ... CAFs were co-cultured overnight with CD4+ T cells at a 1:1 ratio in the presence of pan-HLA antibody (5μg/ml, Biolegend, Cat. No.361702) or GC1q R antibody (10μg/ml ...
-
bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with primary antibodies diluted in 1 % BSA/0.3 % Triton X- 100/DPBS (mouse anti-HA (BioLegend, 901501; 1:1000), mouse anti-myc (NEB/Cell Signaling ...
-
bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
-
bioRxiv - Neuroscience 2021Quote: ... The exception is that secondary labeling was accomplished using anti-rat DyLight 405 (1:5000, Novus, NBPI-72983) or anti-mouse DyLight 405 (1:1000, BioLegend, Poly-24091). Fixed cells were then imaged on a Nikon Eclipse TE2000 inverted microscope using a 60x 1.49NA oil-immersion TIRF objective and 405nm and 560nm solid lasers using a CoolSNAP HQ2 CCD camera ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were immunostained with the following antibodies: rabbit anti-IFITM3 (Cat. nr. AP1153a, Abcepta, 1:500) and mouse anti-HA (clone 16B12, BioLegend, 1:1400). Secondary antibodies conjugated to Alexa® Fluor 488 or 647 (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: ... Livers were sliced into 300μm thick sections on a vibratome and stained with Hoechst (1:2000) and directly conjugated fluorescent antibodies CD31 (Biolegend, 1:100, 390), CD8 (Biolegend ...
-
bioRxiv - Cell Biology 2021Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2022Quote: ... This membrane was blocked with 5% milk and then incubated simultaneously with rabbit polyclonal α-MshA (1:1000, gift of J. Zhu) and mouse monoclonal α-RpoA (1:1000, Biolegend) (as a loading control ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... and 0.3% PBST for 3 days at 4°C on rotating incubator (NFH, 1:500, Abcam, ab4680; Tubulin β-III, 1:200, Biolegend, 801201). Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568 ...
-
bioRxiv - Pathology 2024Quote: ... amyloid-β (clone 6F/3D, 1:500, Dako, Denmark for DLB donors, and clone 4G8, 1:8000, Biolegend, USA for AD donors) and hyperphosphorylated tau (p-tau ...
-
bioRxiv - Pathology 2024Quote: ... then incubated with primary antibody anti-IBA1 (Wako; 011-27991; dilution 1:100) and anti-Amyloid Beta1-42 (Biolegend; 805501; 1:500) prepared in 1% BSA for overnight at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... CD5-APC-eF780 (Thermo Fischer Scientific, Catalog No. 47-0015-82, 1/500) and Streptavidin-BV510 (Biolegend, Catalog No. 405234, 1/500). After final washes ...
-
bioRxiv - Biochemistry 2023Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
-
bioRxiv - Immunology 2023Quote: ... CAR-T cells (1×105 cells/well) and Nalm6-GD2 (1×105 cells/well) were plated in Nalm6-GD2 culture medium containing 1×Brefeldin A (Biolegend, Cat# 420601) for 5-7h ...
-
bioRxiv - Immunology 2024Quote: ... for an additional 4h to allow the accumulation of intracellular cytokines and CD137 APC (1:100; Biolegend, clone 4B4-1, Cat# 309810) to prevent re-internalization.
-
bioRxiv - Cell Biology 2024Quote: ... the samples were incubated in 3% BSA for 1 hr before being treated overnight at 4°C with the respective primary antibodies : PAX6 for ectoderm (1:300; BioLegend, PRB-278P), Brachyury for mesoderm (1:300 ...
-
bioRxiv - Microbiology 2020Quote: ... and/or monoclonal anti-RNA polymerase (1:1,2000, Biolegend). Western blots were probed with IRDye-conjugated secondary antibodies and visualized with Odyssey CLx imagining system (Li-COR Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 1 µg of anti-CD45RO (clone UCHL1, BioLegend) antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-Pax6 (1:1000 dilution; Cat. #: 901301, Biolegend); rabbit anti-NeuN (1:1000 dilution ...
-
bioRxiv - Cancer Biology 2021Quote: ... and anti-PD-1(CD279)-PE/Cy7 (BioLegend 135216). The myeloid antibody cocktail contained ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-HA (mouse; clone 16B12; 901501; Biolegend;1:500), streptavidin-HRP (Pierce-21140;Thermo Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... FITC anti-human ICAM-1/CD54 (Clone DX2, Biolegend), FITC anti-human Fas/CD95 (Clone DX2 ...
-
bioRxiv - Cell Biology 2020Quote: Mouse anti-beta-actin antibody (clone 2F1-1, Biolegend)