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Citations for BioLegend :
3801 - 3850 of 4492 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in blocking solution (2% BSA in PBS) and immunolabeled with anti-CD24 PerCP/Cy5.5 and anti-CD44 FITC primary antibodies (BioLegend, 1:50 ...
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bioRxiv - Immunology 2022Quote: ... for 30mins at 37°C, washed, resuspended in FACS buffer (PBS, 2% FBS) and co-stained with anti-CD4 (GK1.5, Biolegend), anti-CD62L (MEL-14 ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend). CD14+ monocytes were stimulated for 24 hours with 1ug/ml of LPS ...
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bioRxiv - Microbiology 2022Quote: ... then retro-orbitally injected with 2 μg/mouse of APC anti-mouse CD45 (Biolegend, cat#103112; clone 30-F11) antibody in 50 μL PBS for intravenous labeling of CD45+ circulating lymphocytes 15 minutes prior to euthanization ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated to APC-streptavidin (BioLegend, 405207). After washed twice ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, and 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX (BioLegend) and FcR blocking reagent (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently resuspended in staining buffer (PBS with 0.5% bovine serum albumin and 2 mM EDTA) and Fc-blocked using TruStain FcX reagent (BioLegend). Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Immunology 2023Quote: Surface staining for flow cytometry analysis was performed in PBS supplemented with 2% FBS and Trustain FcX (Biolegend, 422301) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... We determined the amounts of IL-2 in the culture supernatant of activated or naïve CD4+ T cells with an ELISA kit (BioLegend). We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2019Quote: ... Sigma)for one hour at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (BioLegend, San Diego, CA, USA), anti-Cytochrome c ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Immunology 2022Quote: ... Staining was then performed at 4°C for 30 minutes with a surface stain cocktail containing the following antibodies: CD4 APC (BioLegend, cat. 100412, clone GK1.5), CD8 Alexa Fluor 700 (BioLegend ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were double immunostained by incubating overnight at 4°C with a mix of anti-CD4 conjugated with PERCP (BioLegend, San Diego, CA, USA) and anti IL-10 conjugated with PE (BD Biosciences Pharmingen ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were used for these studies: anti-human CD47-APC (1µl/106 cells in 100µl; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3), anti-human CD47-FITC (5µl/106 cells in 100µl ...
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bioRxiv - Immunology 2021Quote: ... Cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend, San Diego, CA, US) of APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Genomics 2021Quote: ... 5% normal goat serum in PBS for 1 hour and incubated at 4°C overnight with primary antibody (Biolegend, cat. 613402, dilution 1:500), then at room temperature for 3 hours with secondary antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was incubated in primary antibody overnight at 4°C in a moist chamber diluted in blocking buffer with the following dilutions: Anti-Tubulin β3 (BioLegend, clone TUJ1, 1:500); Anti-OMP (Santa Cruz ...
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bioRxiv - Molecular Biology 2023Quote: ... Murine anti-V5-tag (clone 7/4, #680602) and rabbit anti-V5-tag (Polyclonal, #903801) antibodies were purchased from BioLegend (San Diego, CA, USA). Anti-V5-tag mAb-magnetic beads (#M167-11 ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... The slides were washed with PBS and incubated for 4 hours at room temperature with rat anti- mouse Ly6G (cone 1A8, BioLegend, San Diego, CA, USA). Slides were washed with PBS and incubated separately with Alexa Fluor 568 goat anti-rabbit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250, pS129 α-synuclein (81A, Biolegend 825701, 1:1000).
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bioRxiv - Bioengineering 2024Quote: ... Cells were stained (30 min, 4°C in the dark) with CD11b (Clone: REA713; Miltenyi or Clone: M1/70; Biolegend, San Diego, CA, USA), CD14 (Clone ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then stained with 100 μL of their respective stains (αCD4-FITC, αCD8-FITC, αCD19-FITC, αCD14-APC; Biolegend, https://www.biolegend.com) at a concentration of 1μg/mL for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were incubated with primary antibodies diluted in 1 % BSA/0.3 % Triton X- 100/DPBS (mouse anti-HA (BioLegend, 901501; 1:1000), mouse anti-myc (NEB/Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... A 100 µL sample of EDTA-anticoagulated whole blood was incubated with 5 µL PE-Cy5 CD45 (HI30 clone, Biolegend, cat. # 304010) and 5 µL of PE-CD66B (Becton Dickinson ...
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bioRxiv - Cancer Biology 2022Quote: ... Livers were sliced into 300μm thick sections on a vibratome and stained with Hoechst (1:2000) and directly conjugated fluorescent antibodies CD31 (Biolegend, 1:100, 390), CD8 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently resuspended in 100 µl of FACS buffer with a secondary anti-mouse-APC antibody (BioLegend, catalogue number-405308). The cells were incubated at 4 °C for 30 minutes on ice and washed with PBS as before ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were resuspended in FACS buffer (PBS, 1% FBS, 1 mM EDTA, 0.02% NaN3) and treated with anti-CD16/CD32 (clone 93, Biolegend 101302, 1:100) for 20 min on ice before staining.
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-myelin-associated glycoprotein (MAG, 1:100, SigmaAldrich MAB1567),chicken anti-glial fibrillary acidic protein (GFAP, 1:500, BioLegend PCK-591P), chicken anti-GFAP (1:500 ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Pathology 2024Quote: ... then incubated with primary antibody anti-IBA1 (Wako; 011-27991; dilution 1:100) and anti-Amyloid Beta1-42 (Biolegend; 805501; 1:500) prepared in 1% BSA for overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor single-cell suspensions were stained with Alexa Fluor 700 anti-CD326 (Ep-CAM) antibody (Biolegend, 118239, clone: G8.8, 1:100 dilution), PE/Cyanine 7 anti-mouse CD31antibody (Biolegend ...
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bioRxiv - Biochemistry 2023Quote: ... The fixed cells were permeabilized with 0.1% Triton-X 100 for 10 min at RT and then incubated with chicken anti-vimentin (1:200, 919101, Biolegend, CA, USA) for 30 min in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: Mice with a human engraftment above 5% measured through flow cytometry using mCD45 (eFluor 450, eBiosciences, 1:100), hCD7 (PE, eBiosciences, 1:25) and hCD45 (Alexa Fluor 647, BioLegend, 1:25) were started on treatment which consisted of 0.50 mg kg−1 of vincristine (1 mg ml−1 injection ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... CD18 (clone CBR LFA-1/2, 366310), ITGB7 (clone FIB504, 121006), and CD68 (clone Y1/82A, 333814) were acquired from Biolegend.
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bioRxiv - Immunology 2021Quote: ... containing the following fluorescently-labelled antibodies diluted in FACS buffer (2% FBS in PBS): CD4-APC-Cy7 (clone A161A1; BioLegend), CD8a-Alexa700 (clone SK1 ...
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bioRxiv - Immunology 2021Quote: 1-2 × 106 fresh decidual leukocytes were washed with PBS and stained using the following cocktail of antibodies: CD45 (HI30, Biolegend), CD66b (G10F5 ...
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bioRxiv - Immunology 2021Quote: ... The coronavirus protein panel consisted of the following flurorescent probes: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), WIV1 RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were discarded and cells were resuspended in 25 µl of staining solution containing the antibodies detailed in Table 2 diluted in 1X live/dead fixable viability dye (Zombie-Aqua, Biolegend) and incubated 30 min at RT in the dark ...