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Citations for BioLegend :
3751 - 3800 of 4330 citations for Beta NGF Mouse 110a.a since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the cell pellet was resuspended in 1% BSA/ PBS containing secondary fluorescence antibody (PE anti-mouse IgG1, BioLegend, 406608) and incubated for 1 h as described above ...
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bioRxiv - Neuroscience 2023Quote: ... 17-24 Antibody (1:1000, Clone 4G8, N-terminal, a.a. 17-24, human & mouse specific, 800708 BioLegend, CA, USA); Amyloid Precursor Protein (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with Alexa Fluor® 700 anti-mouse CD206 (MMR) antibody (1:200; Biolegend, C068C2) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Cancer Biology 2023Quote: Single-cell suspensions from tumors or CD3 T cells isolated using MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend) following the manufacture’s protocols ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of mouse cell-surface molecules: ① Purchased from Biolegend(USA): Anti-CD45-BV510(clone 30-F11);Anti-Ly-6G/Ly-6c(Gr-1)-PerCP(clone RB6-8C5);Anti-CD4-BV421(clone GK1.5);Anti-F4/80-FITC(clone BM8);Anti-CD8a-PE(clone 53-6.7) ...
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bioRxiv - Immunology 2023Quote: ... cells treated with a Foxp3/Transcription factor staining buffer kit (Cat. TNB-0607, TOMBO Bioscience) were stained with FITC-labeled anti-mouse CD4 Ab (GK1.5, BioLegend) and allophycocyanin-labeled anti-mouse Foxp3 Ab (3G3 ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For the HashTag library TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody (Clone M1/42; 30-F11; 155835; BioLegend) was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42 ...
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bioRxiv - Immunology 2023Quote: ... and around 1×106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2023Quote: ... congenic CD45.1 mouse spleen was isolated and red blood cells were lysed with RBC lysis buffer (BioLegend, CA, USA). Leukocytes were then resuspended in complete RPMI with 1µg/mL high molecular weight Poly(I:C) ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for immunofluorescence were: mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), Rabbit Anti-Aβ (6E10 clone ...
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bioRxiv - Immunology 2023Quote: Cells harvested from in vitro Th17 differentiated cells were stained with the following antibodies against mouse CD3 (17A2, Biolegend), CD4 (GK1.5 ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed on ice for 1 hour with APC/Cy7 anti-mouse CD45 (clone 30-F11; BioLegend), rat monoclonal FITC anti-mouse Ly6B.2 (clone 7/4 ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse lung fibroblasts were freshly isolated after magnetic bead-based negative selection of epithelial cells (118204, Biotin anti-mouse CD326 Epcam, Clone: G8.8, BioLegend), endothelial cells (102404 ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Two million viable cells were resuspended in 100µl FACS buffer with 1 µl of Fc Block (anti-mouse Fc block CD16/32, Cat# 101302, Biolegend) for 20 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... immunohistochemistry (IHC) and flow-cytometry (FC): Pacific Blue anti-mouse Ly-6A/E (Biolegend, Cat#108120, 1:50 FC), Sca-1/Ly6A/E (E13 161-7 ...
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bioRxiv - Microbiology 2023Quote: ... T lymphocytes were evaluated using the following antibodies: rat anti-mouse IL-17A (TC11-18H10.1, 1/50; Biolegend, #506922), rat anti-mouse RORγt (AFKJS-9 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were stained with the following monoclonal antibodies to determine cellular architecture of most abundant spleen and immune cells (Table S10): anti-mouse CD45.2 (Biolegend), anti-mouse Gr-1 (eBioscience) ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Immunology 2024Quote: ... lymph node suspension from 2 mice per biological replicate were activated with 0.5 µg/mL anti-mouse CD3 (Biolegend) and 0.5 µg/mL anti-mouse CD28 (eBioscience ...
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bioRxiv - Cancer Biology 2024Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control, APC Mouse IgG2a, k; #400220, Biolegend) or Anti-human/mouse CD49f antibody (#313620 ...
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the femurs of MMTV-PyMT tumour bearing mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies used were as follows: anti-βIII tubulin (rabbit, 1:5000, BioLegend, 801201; mouse, 1:3000, BioLegend, 801213), anti-MBP (mouse ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies were used for immunoblotting: mouse monoclonal antibodies against TuJ1 (BioLegend Cat# 801202 (also 801201, 801213), RRID:AB_10063408 ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then placed in primary antibody solution containing 1:1000 mouse anti-SMI-32 (BioLegend, Catalog #: 801701, RRID:AB_509997) in 3% normal donkey serum in 0.3% Triton PBS for 2-4 days at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the blood of MMTV-PyMT tumour bearing mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were first incubated with True Stain fcXTM antibody (anti-mouse CD16/32, BioLegend, #101319, clone 93, 1:100) in PBS containing 0.5% BSA and 2 mM EDTA before staining with antibodies directly conjugated to fluorophores for flow cytometry ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST) and incubated overnight with primary antibodies: mouse anti-STAT1 (1:800, Cat# 603702, Biolegend), mouse anti-STAT1 phospho (Ser727 ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected (spun down at 10,000 x g for 15 mins) for cytokine analysis using LegendPlex 13-plex Mouse Inflammation Panel (Biolegend). Adhered cells were washed with 1x PBS and collected using 0.25% trypsin ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used for staining mouse tissue sections for imaging or isolated cells for flow cytometry: B220 PCPCy5.5 (clone RA3-6B2; Biolegend), B220 PE/Fire 700 (clone RA3-6B2 ...
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bioRxiv - Immunology 2024Quote: Proinflammatory chemokines of normal liver and liver micrometastasis were detected using LEGENDplex™ Mouse Proinflammatory Chemokine Panel (BioLegend, #740451). Briefly ...
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bioRxiv - Microbiology 2024Quote: Western and immunofluorescence assays (IFA) were probed using the following antibodies: mouse monoclonal antibody (mAb) HA.11 (Biolegend; 901515), rabbit polyclonal antibody (pAb ...
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bioRxiv - Neuroscience 2024Quote: ... cells were placed on ice for 15 mins and treated with cold Mouse monoclonal antibody against LAMP1(#328611; Biolegend) specific to the N terminal luminal epitope of the antigen ...
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bioRxiv - Immunology 2024Quote: Liver cells were collected by centrifugation and then incubated with Fc blocking antibody (purified anti-mouse CD16/32, BioLegend) on ice for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... washed and re-suspended in the following primary antibodies: a rat monoclonal (IgG2a) anti-mouse FRβ (BioLegend,1:100) or a sheep polyclonal anti-mouse FRα (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: Skin cytokines or growth factors were quantified using LegendPlex Mouse Cytokine release syndrome and Hematopoietic stem cell kits (Biolegend). Briefly ...
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bioRxiv - Immunology 2024Quote: ... cells were resuspended in 50 µL Fc blocking medium (2.5 µg/ml purified anti-mouse CD16/32 Antibody (BioLegend) in Cell Staining Buffer (BioLegend)) ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ CD25- and CD8+ T cells were purified using the MojoSort Mouse CD4 (supplemented with biotinylated anti-CD25) and CD8 T Cell Isolation Kits (BioLegend), respectively ...