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Citations for BioLegend :
3701 - 3750 of 10000+ citations for Rabbit Anti Human IgG Fc Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... T cells were separated from PBMCs using a MojoSort human CD3+ T cell isolation kit (Biolegend, USA).
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301, all human samples) and incubated for 3 min on ice with periodic shaking ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT after adding Human TruStain FcX (1:200 dilution, Biolegend, San Diego, CA) for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend). Readings were made on an AttuneTM Nxt Flow Cytometer.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were randomized on the assay plates and analyzed using the LEGENDplex human CD8/NK panel (BioLegend). Samples were read on a CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... GFAP (rabbit, Dako Z0334, 1:1000; mouse, Biolegend SM121, 1:500). For fluorescent stainings ...
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bioRxiv - Developmental Biology 2021Quote: ... PAX6 primary antibody (Rabbit α PAX6 Biolegend cat. no. 901301, lotB277104) was diluted 1:100 in 0.5% BSA and samples were incubated for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... were functionalized with rabbit polyclonal GFP antibody (BioLegend, no. MMS-118P), as previously described31 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: Pax6 (Rabbit, 1:200, Biolegend), Tuj1 (Mouse ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-labeled antibodies anti-CD3-PE and anti-CD4- or anti-CD8-FITC (BioLegend) either in FACS buffer (PBSO ...
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bioRxiv - Immunology 2021Quote: ... anti-CD28 and anti-CD40 (3 μg/mL, Biolegend) in a round-bottom 96-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD137 Alexa647 or anti-CD137 PE-Cy7 (Biolegend) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD103 PE-Cy7 and anti-CD25 PE (Biolegend), and with anti-CD8 BUV805 ...
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bioRxiv - Immunology 2022Quote: ... APC-anti-TNFα (eBioscience) and BV711-anti-CD8 (Biolegend). Cells were fixed with BD CellFix and run on an Attune NXT Flow Cytometer (Thermofisher).
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bioRxiv - Immunology 2022Quote: ... anti-CD38-PECy7 and anti-CD45.1-APC from Biolegend. eFluor780 from Thermofisher was used for viability ...
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bioRxiv - Immunology 2020Quote: ... anti-CD88 anti-APC (1:800, 20/70, Biolegend), anti-MHC-II APC-ef780 (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... anti-CD8 (as above) and anti-IFNγ (XMG1.2, BioLegend), stained intracellularly[17] ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific antibody was detected using biotinylated anti-mouse antibodies (anti-IgG1 [RMG1-1] 0.02μg/ml, anti-IgG2b [RMG2b-1] 0.05μg/ml, Biolegend, CA ...
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bioRxiv - Cell Biology 2021Quote: ... at a final concentration of 2.5μg/ml and we run in parallel samples with IgG isotype control (BioLegend, Cat#400526). All antibodies were centrifuged for 10 min at 14,000 x g before use ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: IL-21 concentration from tonsil mono-cultures was measured using Human IL-21 ELISA MAX Deluxe kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... Samples were thawed on ice prior to detection and analysis using LEGENDplex human hematopoietic stem cell panel (BioLegend), according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked in 1X PBS containing 1 mg/ml BSA and 2.5 µl of human FcX-TruStain (BioLegend Inc.) for 15 minutes and incubated with PE-c-CASPASE3 antibody (Cell Signaling #9978 ...
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bioRxiv - Immunology 2020Quote: ... Human trachea was stained for 72 hours in PBS serum and the following antibodies: hCD103-AlexaFluor 594 (Biolegend) 1:40 ...
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bioRxiv - Immunology 2019Quote: ... Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend) and data acquired on a BD Symphony A5 cytometer ...
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bioRxiv - Immunology 2019Quote: The following fluorescent conjugated antibodies were used: antibodies to human CD3ε-BUV395 or PeCy7 (UCHT1; eBioscience and Biolegend), CD4-PerCPCy5.5 (RPA-T4) ...
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bioRxiv - Immunology 2019Quote: ... Human MAIT cells were isolated by sorting CD2 MACS-enriched (Miltenyi) leukocytes with CD161 and Vα7.2 antibodies (BioLegend). MAIT cells were grown for around 6 weeks in complete RPMI media supplemented with IL-2 ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Immunology 2022Quote: Secreted cytokines were assessed with LEGENDplex Human Th Panel 12-plex and analysed with Qognit software (both BioLegend). For cytokine secretion by Tregs ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in saliva were measured twice per sample using the LEGENDplex Human Essential Immune Response panel (BioLegend), as described in previous studies 26,57 ...
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Cytokines in the cell culture supernatants were quantified using Human Th Cytokine Panel (12-plex) (Cat. 741027, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cytokines were quantified using the LEGENDplex Human CD8/NK Panel 13-plex (Cat.No # 740267 Lot B330268) from Biolegend. This is a bead-based immunoassay able to quantify the concentration of different analytes in co-culture supernatants ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted again and resuspended in 100 μl of 1:10 dilution of Human TruStain FcX (Biolegend) in MACS buffer ...
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bioRxiv - Immunology 2023Quote: Surface expression of mouse or human CD47 on murine tumor cell lines was assessed using MIAP301-PE (Biolegend) and CC2C6-APC (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: Determination of human α-synuclein concentration in nodose ganglia was performed according to manufacter’s instructions (Biolegend, Cat#844101). The 1:10 diluted samples were run in triplicates and reference standard curve ranged from 6.1 – 1500 pg per mL ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: Listed below are the antibodies used for flow cytometric analysis of human cellsurface molecules: ①Purchased from Biolegend(USA): Anti-hCD36-PE(clone 5271).
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...