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Citations for BioLegend :
3601 - 3650 of 3691 citations for Recombinant Human TNFRSF4 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Tissue cytokines in the heart protein extracts were assayed using the mouse Th2 Panel LEGENDplex assay (BioLegend), according to the manufactures instructions.
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bioRxiv - Immunology 2020Quote: ... Supernatants were normalised to protein concentration before being subject to an ELISA protocol or TNF-α (Biolegend), IL-12 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and detection of indicated proteins was carried out using the following antibodies: Eos (W15169A; BioLegend, 1:500), pY-STAT5 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: Oligonucleotides were conjugated to two antibodies against HGSOC cell surface proteins β2-Microglobulin (β2M) and CD298 (BioLegend) by iEDDA-click chemistry according to CITE-seq antibody-oligo conjugation protocol (https://cite-seq.com) ...
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Blocking antibodies specific for the following proteins were used: 2.5 μg/ml CD11b (M1/70, BioLegend, #101224), 2.5 μg/ml CD11c (N418 ...
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bioRxiv - Cancer Biology 2023Quote: CD41 and CD62p proteins on platelets were stained with FITC conjugated anti-CD41 (BioLegend 303704,1:50 dilution) and PE conjugated anti-CD62p (BD Pharmingen 561921 ...
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bioRxiv - Immunology 2023Quote: ... The gels were transferred to nitrocellulose and Mab-associated proteins were detected with anti-C3 (BioLegend, 846302), -C1q (Novus ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for the following 5h using a combination of protein transport inhibitor (Monensin) (Biolegend, USA), Ionomycin (1 ug/ml) ...
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bioRxiv - Microbiology 2019Quote: ... mice were intravenously injected with 15 μg of mouse anti-human CD31 coupled to Alexa-Fluor-647 (clone WM59, Biolegend, San Diego, CA, United States) approximately 2 hrs prior to imaging.
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... spleen and bone marrow were isolated and evaluated for the percentage of human CD45+ cells by flow cytometry (CD45-PerCP-Cy5.5, Biolegend Cat: 304028; BD FACS Canto II). Populations were defined and analyzed using FlowJo software (Flowjo v10.7.1 ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... The bone marrow was harvested from each mouse and the infiltrated CCRF cells were identified by staining for a human CD45 antibody (Biolegend; AB_2687375; BLG-368522; 1:200).
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bioRxiv - Systems Biology 2023Quote: ... the four-class iPSC dataset (Cell, Mitotic, Dying cell, Debris) was stained with PE anti-human TRA-1-81 (1:300, Biolegend, #330708, clone TRA-1-81), while the five-class iPSC morphology dataset was stained with Alexa Fluor ® 555 anti-GSK3 beta (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... we collected the supernatants and quantified the concentrations of each cytokine using the LEGENDplex™ Human Essential Immune Response Panel (13-plex) (BioLegend, San Diego, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Immunology 2023Quote: ... Cells were analyzed and sorted for transfection efficiency via flow cytometry analysis using anti-human CD3 APC/Cy7-conjugated antibody and anti-human CD34 PE-conjugated antibody for TCR-expressing cells (BioLegend, 300426 at 10 μg/mL and BioLegend, 343606 at 1.25 µg/mL).
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for B cells: anti-CD19 Alexa Fluor® 488 (BioLegend catalog #302219, clone HIB19), anti-CD3 PerCP/Cy5.5 (TONBO catalog #65-0037-T100 ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the PBMC staining panel for NK cells: (donors 1-2) anti-IFN-γ FITC (BioLegend catalog #506504, clone B27), anti-CD19 PerCP/Cy5.5 (BioLegend catalog #309504 ...
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bioRxiv - Molecular Biology 2021Quote: ... and the hybrid protein in the brain was done using these antibodies: α-Aβ (Biolegend clone 6E10 #803017), α-MAP2 (ThermoFisher #MA5-12826) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein bands were either visualized by Comassie stain or by standard Western blot procedure (anti-ubiquitin antibody BioLegend, Cat Number ...
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bioRxiv - Immunology 2020Quote: Antibodies and clones reactive against the following proteins were used: CD2 (TS1/8, BV421 or Pacific Blue, Biolegend), CD3 (UCHT1 ...
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bioRxiv - Microbiology 2020Quote: ... rpoS protein was probed by anti-mouse IgG anti-E.coli RNA sigmaS clone:1RS1 antibody (Biolegend; batch-B274469). Protein gel was cut at 50KDa ladder mark ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies and dilutions were used: rabbit anti-transformation related protein 63 (P63, 1:250, 619002, Biolegend), rabbit anti-red fluorescent protein (tdT ...
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bioRxiv - Genetics 2023Quote: ... proteins were transferred onto nitrocellulose membranes and probed with a 1:2,000 dilution of anti-RpoS antibody (Biolegend). For secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... the cultured cells were harvested and incubated with either fluorescein isothiocyanate (FITC)-conjugated anti-human FR-β antibody (m90916, a gift from Philip S. Low) or allophycocyanin (APC)-conjugated anti-human FR-β antibody (mouse IgG2a, BioLegend, San Diego, CA, USA), and isotype controls (mouse IgG-FITC ...
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bioRxiv - Genomics 2019Quote: ... cells were washed once in PBS and once in Flow buffer (PBS with 2% BSA) and stained with anti-human CD47-APC antibody (clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3) in Flow buffer (20µl antibody/40×106 cells/ml ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of TNF-α mRNA and protein were detected by qPCR (Table 1) and ELISA (BioLegend, San Diego, CA), respectively.
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Pathology 2022Quote: ... was coated with 1.2 μg/mL HERV-K113 Capsid protein (sp|Q902F9|90-632, Genescript) diluted in ELISA coating buffer (421701, Biolegend) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend, 101251, clone M1/70), CD45-Percp-Cy5.5 (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... Experimental values are expressed as a ratio of protein levels over control with the TFIIB (Biolegend, 1:2000 dilution) and Histone H3 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... staining and neutrophil purity was assessed using a Benton Dickson LSR Fortessa X-20 flow cytometer via distinct forward and side scatter profiles or CD66b-peridinin chlorophyll protein (PerCP)/Cy5.5 (clone G10F5, BioLegend) expression ...
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bioRxiv - Genomics 2021Quote: ... and then incubated 1:100 with antibodies directed against cell surface proteins CD11b-BV421 (BioLegend 101251, clone M1/70), CD45-APC (BioLegend 103112 ...
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bioRxiv - Bioengineering 2020Quote: ... recombinant AAV9 (2×109 vg/well) and SpCas9 protein (0.27 μg/well) were diluted in 1x Coating Buffer A (BioLegend) and used to coat a 96-well Nunc MaxiSorp plate ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Cell Biology 2024Quote: ... The expression level of specific proteins was detected by immunoblot analysis with the following antibodies: HA (BioLegend, MMS-101R), V5 (Thermo Fisher Scientific R96025) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... Eluted fractions were pooled and purified proteins were verified for integrity by probing with an anti-HIS tag antibody (Biolegend) as well as with subtype-specific mAbs via SDS-PAGE and Western blot.
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bioRxiv - Immunology 2021Quote: ... Antibodies for flow cytometry and cell isolation against the following proteins (all mouse unless otherwise indicated) were obtained from BioLegend, eBioscience ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... activated T-cells were treated with HDACI as described before and after 72 h total protein acetylation of the cells was determined by flow cytometry using PE-coupled anti-Acetylated Lysine Antibody (Biolegend). Cells were washed with PBS ...